Paradoxical Sleep Deprivation Increases the Content of Glutamate and Glutamine in Rat Cerebral Cortex

[en] We investigated the influence of the sleep/waking cycle, the effects of paradoxical sleep deprivation (PSD) and of the vigilance-promoting drug modafinil on the amino acid contents of rat brain cortex. No significant nycthemeral variations in amino acid levels could be detected. PSD (12-24 hours), using the water tank method, significantly increased the levels of glutamate and glutamine. The increase was still observed after the sleep rebound period. gamma-Aminobutyric acid (GABA) levels did not change significantly during the instrumental sleep deprivation but increased during the rebound period. Control experiments indicate that the increase in glutamate and glutamine levels is due to PSD rather than to the stress associated with the experimental procedure. The increase in glutamate content cannot arise only from transamination reactions, because the levels of other amino acids (such as aspartate) did not decrease. Modafinil treatment did not significantly modify the brain cortex content of any of the amino acids tested.

Disciplines :

Biochemistry, biophysics & molecular biology

Author, co-author :

Bettendorff, Lucien ; Université de Liège - ULiège > Département des sciences biomédicales et précliniques > Biochimie et physiologie humaine et pathologique

Sallanon-Moulin, M.

Touret, Monique

Wins, Pierre

Margineanu, Ilca

Schoffeniels, Ernest

Language :

English

Title :

Paradoxical Sleep Deprivation Increases the Content of Glutamate and Glutamine in Rat Cerebral Cortex

Publication date :

1996

Journal title :

Sleep

ISSN :

0161-8105

eISSN :

1550-9109

Publisher :

The American Academy of Sleep Medicine, United States - Illinois

Volume :

Issue :

Pages :

65-71

Peer reviewed :

Peer Reviewed verified by ORBi

Available on ORBi :

since 23 January 2009

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