Solubilization of Sodium Channel from Human Brain

De Rycker, C.; Grandfils, Christian; Bettendorff, Lucien; Schoffeniels, Ernest

doi:10.1111/j.1471-4159.1989.tb09128.x

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Solubilization of Sodium Channel from Human Brain

De Rycker, C.; Grandfils, Christian; Bettendorff, Lucien et al.

1989 • In Journal of Neurochemistry, 52 (2), p. 349-53

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https://hdl.handle.net/2268/4572

DOI
10.1111/j.1471-4159.1989.tb09128.x

PubMed
2536069

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Abstract :

[en] [3H]Tetrodotoxin binds to a single class of receptor sites in homogenates of human brain with a KD of 9.1 nM at 0 degree C and a maximal binding capacity of 5.9 pmol/mg of protein. This tetrodotoxin receptor has been solubilized, and several parameters influencing the efficiency of this critical step have been studied. Treatment of brain membranes with 2% (wt/vol) Nonidet P-40 solubilizes up to 38% of the tetrodotoxin receptor sites. The duration of this solubilization step must not exceed 15 min at an optimal pH of 6.8. The binding activity is most stable when exogenous phosphatidylcholine is added to the soluble receptor with a phosphatidylcholine/detergent ratio of 1:5.

Disciplines :

Biochemistry, biophysics & molecular biology

Author, co-author :

De Rycker, C.

Grandfils, Christian ; Université de Liège - ULiège > Biochimie et physiologie générale

Bettendorff, Lucien ; Université de Liège - ULiège > Département des sciences biomédicales et précliniques > Biochimie et physiologie humaine et pathologique

Schoffeniels, Ernest

Language :

English

Title :

Solubilization of Sodium Channel from Human Brain

Publication date :

1989

Journal title :

Journal of Neurochemistry

ISSN :

0022-3042

eISSN :

1471-4159

Publisher :

Blackwell, Oxford, United Kingdom

Volume :

Issue :

Pages :

349-53

Peer reviewed :

Peer Reviewed verified by ORBi

Available on ORBi :

since 23 January 2009

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Bibliography

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