Aerosol Deposition in Equine Lungs Following Ultrasonic Nebulisation Versus Jet Aerosol Delivery System

[en] Therapeutic aerosols pay an increasing role in the treatment of equine respiratory disorders. This route of delivery permits concentration of significant amounts of drugs at the site of action without unwanted high systemic concentration and resultant side effects. The efficiency of such a topical therapy depends on the quantity of inhaled drugs deposited in the lungs and, for some drugs, on the proportion retained in specific parts of the lungs. The objective of this study was to define and to compare quantitative (dose deposited) and qualitative (regional distribution) deposition of an aerosol in the equine lungs, using either a ultrasonic nebuliser (UN) currently used in human medicine or a high pressure jet nebuliser (JN) especially developed for the equine species. This comparison was possible owing to gamma-scintigraphy, a noninvasive technique ideally suited to give information about both total and regional deposition of inhaled drugs in the respiratory tract. The quantitative study did not point out any difference between the 2 systems concerning the activity released from the nebuliser proportionally to the initial loaded dose (mean +/- s.d. 45.95 +/- 4.93% for the UN vs. 46.47 +/- 8.49% for the JN). By contrast, the percentage of the dose released reaching the lungs was significantly lower with the UN compared to the JN (5.09 +/- 0.66% vs. 7.35 +/- 1.96%). The qualitative analysis did not show any significant difference in size of aerosol deposition image between the 2 nebulisers. However peripheral deposition was significantly higher with JN compared to UN. In conclusion, both nebulisers may be used for aerosol therapy in the equine species. The ultrasonic and pneumatic nebulisation achieved drug deposition in the peripheral part of the lungs (i.e. small airways and lung parenchyma).

Disciplines :

Veterinary medicine & animal health

Author, co-author :

Votion, Dominique ; Université de Liège - ULiège > Département clinique des animaux de compagnie et des équidés > Anesthésiologie gén. et pathologie chirurg. des grds animaux

Ghafir, Y.

Munsters, K.

Duvivier, D. H.

Art, Tatiana ; Université de Liège - ULiège > Département de sciences fonctionnelles > Phys. neuro-muscul., de l'effort - Méd. sport. des animaux

Lekeux, Pierre ; Université de Liège - ULiège > Département de sciences fonctionnelles > Physiologie

Language :

English

Title :

Aerosol Deposition in Equine Lungs Following Ultrasonic Nebulisation Versus Jet Aerosol Delivery System

Publication date :

September 1997

Journal title :

Equine Veterinary Journal

ISSN :

0425-1644

eISSN :

2042-3306

Publisher :

Equine Veterinary Journal Ltd, Newmarket, United Kingdom

Volume :

Issue :

Pages :

388-393

Peer reviewed :

Peer Reviewed verified by ORBi

Available on ORBi :

since 21 January 2009

Statistics

Number of views

144 (10 by ULiège)

Number of downloads

3 (3 by ULiège)

More statistics

Scopus citations^®

Scopus citations^®
without self-citations

OpenCitations

Bibliography

Agnew, J.E. (1984a) Physical properties and mechanisms of deposition of aerosols. In: Aerosols and the Lung: Clinical and Experimental Aspects Eds: S.W. Clarke and D Pavia. Butterworth and Co, Inc., London, pp 49-70.
Agnew, J.E. (1984b) Aerosol contributions to the investigation of lung structure and ventilatory function. In: Aerosols and the Lung: Clinical and Experimental Aspects Eds: S.W. Clarke and D. Pavia. Butterworth and Co, Inc , London, pp 92-126.
Amis, T.C., Pascoe, J.R. and Homof, W. (1984). Topographical distribution of pulmonary ventilation and perfusion in the horse. Am. J. vet Res 45, 1597-1601
Atkins, P J., Barker, N P and Mathisen, D. (1992) The design and development of inhalation drug delivery systems. In: Pharmaceutical Inhalation Aerosol Technology. Ed: A.J. Hickey Marcel Decker Inc., New York pp 155-185.
Ballinger, J R , Andrey, T W., Boxen, I. and Zhang, Z.M. (1993) Formulation of Technetium-99m aerosol colloid with improved delivery efficiency for lung ventilation imaging. J. nucl. Med 34, 268-271.
Beck, K.C. (1987) Regional trapping of microspheres in the lung correlates well with regional blood flow. J. appl. Physiol 63, 883-889.
Brain, J.D. and Valberg, P.A. (1979) Deposition of aerosol in the respiratory tract. Am. Rev. respir Dis 120, 1325-1373.
Broadstone, R V., LeBlanc, PH , Derksen, F.J. and Robinson, N.E. (1991a) In vitro responses of airway smooth muscle from horses with recurrent airway obstruction Pulm Pharmacol 4, 191-202.
Broadstone, R.V., Robinson, N E , Gray, P.R., Woods, P S A. and Derksen, F J. (1991b) Effects of furosemide on ponies with recurrent airway obstruction Pulm. Pharmacol 4, 203-208.
Broadstone, R.V., Gray, P.R., Robinson, N E., Derksen, F.J (1992) Effects of xylazine on airway function in ponies with recurrent airway obstruction Am. J. vet. Res 53, 1813-7
Chamberlain, M J , Morgan, W.K.C. and Vinitski, S (1983) Factors influencing the regional deposition of inhaled particles in man. Clin. Sci. 64, 69-78
Clarke, A F. (1991) Practicalities of nebulisation Equine vet. Educ. 3, 45-49.
Clay, M M. and Clarke, S.W (1987) Effect of nebulised aerosol size on lung deposition in patients with mild asthma. Thorax 42, 190-194.
Davis, S.S., Hardy, J.G , Newman, S.P. and Wilding, I R. (1992) Gamma scintigraphy in the evaluation of pharmaceutical dosage forms. Eur. J. nucl Med. 19, 971.
Dennis, J.H. (1995) Drug nebuliser design and performance: breath enhanced jet vs Constant output jet vs. Ultrasonic J. Aerosol Med 8, 277-280
Derksen, F.J., Robinson, N E and Berney C.E. (1992) Aerosol pirbuterol: bronchodilator activity and side effects in ponies with recurrent airway obstruction (heaves). Equine vet. J. 24, 107-112
Derksen, F.J , Olszewski, M., Robinson, N.E., Berney, C.E , Lloyd, J.W., Hakala, J , Matson, C. and Ruth, D. (1996) Use of a hand-held, metered-dose aerosol delivery device to administer pirbuterol acetate to horses with 'heaves' Equine vet. J. 28, 306-310.
Dixon, P.M. (1992) Therapeutics of the respiratory tract In: Current Therapy in Equine Medicine 3. Ed N.E.Robinson W B. Saunders Company, Philadelphia pp 303-310
Dolovich, M (1993) Lung dose, distribution, and clinical response to therapeutic aerosols. Aerosol Sci. Technol 18, 230-240
Duvivier, D.H , Art, T., Votion, D and Lekeux, P. (1995) Bronchodilating effects of ipratropium bromide inhalation in COPD horses: a dose finding study In Proceedings: Drug Delivery to the Lungs VI, London. pp 44-48.
Flament, M.P , Leterme, P and Gayot, A.T (1995) Factors influencing nebulising efficiency. Drug Dev. Ind Pharm. 21, 2263-2285.
Genicot, B , Peckova, M , Close, R., Lindsey, J.K., Lambert, P and Lekeux, P (1994) Technical study of some major parameters influencing the performances of an aerosol delivery equipment suitable for drug inhalation in calves. Vet. Res 25, 468-177
Genicot, B , Lapp, K , Close, R., Lindsey, J.K , Lambert, P and Lekeux, P (1995) Physical properties of particles of ipratropium and clenbuterol generated by equipment suitable for the inhalation of drugs by calves. Res vet. Sci 59, 164-167.
Hardy, J.G., Newman, S P. and Knoch, M. (1993) Lung deposition from four nebulisers. Respir Med 87, 461-465.
Hardy, J.G , Everard, M.L., Coffiner, M and Fossion, J. (1993) Lung deposition of a nacystelyn metered dose inhaler formulation. J. Aerosol Med. 6, 37-42.
Ilowite, J S , Baskin, M.I., Sheetz, M.S. and Abd, A G. (1991) Delivered dose and regional distribution of aerosolized pentamidine using different delivery systems. Chest 99, 1139-1144.
Johnson, M.A, Newman, S P., Bloom, R , Talaee, N and Clarke, SW. (1989) Delivery of Albuterol and Ipratropium Bromide from two nebuliser systems in chronic stable asthma. Chest 1, 6-10
Lal, S., Harris, D.M., Bhalla, K K , Singhal, S N and Butler, A.G. (1972) Comparison of beclomethasone diproprionate aerosol and prednisolone in reversible airways obstruction. Br med J. 3, 314-320.
Lavoie, J.P., Phan, S T. and Blais, D. (1996) Effects of a combination of detonidine and butorphanol on respiratory function in horses with or without chronic obstructive pulmonary disease. A J.V.R. 57, 705-709.
LeBlanc, H., Broadstone, R V , Derksen, F.J and Robinson, N E (1991) In vitro responses of distal airways in horses with recurrent airway obstruction. Am. J vet. Res. 52, 999-1003
Lippmann, M , Yeates, D.B and Albert, R.E. (1980) Deposition, retention and clearance of inhaled particles. Br. J ind Med. 37, 337-362
Mason, D E , Muir, WW. and Olson, L.E (1989) Response of equine airway smooth muscle to acetylcholine and electrical stimulation in vitro. Am J. vet Res. 50, 1499-1504.
Miki, M., Isawa, T., Teshima, T , Anazawa, Y. and Motomiya, M (1992) Difference in inhaled aerosol deposition patterns in the lungs due to three different sized aerosols Nucl Med Commun. 13, 553-562.
Murphy, J R., McPherson, E A and Dison P. (1980) Chronic obstructive pulmonary disease (COPD): effects of bronchodilatator drugs on normal and affected horses. Equine vet. J. 12, 10-14.
Newman, S.P. (1984) Production of radioaerosols. In Aerosols and the Lung. Clinical and Experimental Aspects. Eds: S.W Clarke and D. Pavia. Butterworth and Co, Inc., London pp 71-91.
Newman, S.P. (1985) Aerosol deposition considerations in inhalation therapy. Chest 88, 152S-160S
Newman S P. (1993) Scintigraphic assessment of therapeutic aerosols Crit. Rev Ther. Drug Carrier Syst. 10, 65-109.
Newman, S.P., Pavia, D., Moren, F., Sheahan, N F. and Clarke, S.W. (1981) Deposition of pressurized aerosols in the human respiratory tract. Thorax 36, 52-55.
Newman, S.P , Pellow, P.G.D. and Clarke, S.W. (1987) In vitro comparison of DeVilbiss jet and ultrasonic nebulisers. Chest 92, 991-994.
O'Callaghan, M.W. (1991) Nuclear imaging techniques for equine respiratory disease. Vet. Clin. N. Am.: Equine Pract 7, 417-33.
O'Callaghan, M.W , Hornof, W.J., Fisher, P.E. and Rabbe, O.G. (1987) Ventilation imaging in horse with 99m-Technetium-DTPA radioaerosol. Equine vet J. 19, 19-24.
Olson, L.E., Perkowski, S Z , Mason, D.E. and Muir, WW. (1989a) Epithelium-and mucosa-dependent relaxation and contraction of normal equine trachealis muscle in vitro. Am. J. vet. Res. 50, 1720-1724.
Olson, L.E., Perkowski, S.Z., Mason, D.E. and Muir, W.W. III (1989b) Isoproterenol- and salbutamol-induced relaxation of acetylcholine- and histamine-induced contraction of equine trachealis muscle in vitro. Am. J. vet. Res. 50, 1715-1719.
Pavia, D., Thomson, M L , Clarke, S.W and Shannon, H.S. (1977) Effect of lung function and mode of inhalation on penetration of aerosol into the human lung. Thorax 32, 194-197.
Phipps, P.R. and Gonda, I (1994) Evaporation of aqueous aerosols produced by jet nebulisers: effects on particle size and concentration of solution in the droplets. J. aerosol Med. 7, 239-258.
Phipps, P.R , Gonda, I , Anderson, S.D., Bailey, D. and Bautovich, G. (1994) Regional deposition of saline aerosols of different tonicities in normal and asthmatic subjects Eur. Respir. J. 7, 1474-1482.
Plit, M., Goldman, H.I., Cassel, M L and Zwi, S. (1972) The bronchodilatator action of fenoterol (Berotec) in asthma Med. Proc. 18, 41.
Reitemeyer, H., Klein, H.J., Deegen, E. (1986) The effect of sedatives on lung function in horses. Acta vet. Scand. 82, 111-20.
Robinson, N.E. (1993) Rôle du système nerveux autonome dans l'obstruction des voies aériennes du cheval Ann. Méd. Vét 137, 153-159
Robinson, N.E., Derksen, F J , Berney, C. and Goossens, L. (1993) The airway response of horses with recurrent airway obstruction (heaves) to aerosol administration of ipratropium bromide Equine vet. J. 25, 299-303.
Short, M.D., Singh, C.A., Few, J D., Styddy, P.R , Heaf, P J.D. and Spiro S.G (1981) The labelling and monitoring of lung deposition of an inhaled synthetic anticholinergic bronchodilating agent. Chest 80, S918-S924.
Soma, L.R., Beech, J and Gerber, N.H. (1987) Effects of cromolyn in horses with chronic obstructive pulmonary disease. Vet. Res Commun. 11, 339-351
Spiro, S.G., Singh, C.A., Tolfree, S E.J , Partridge, M R and Short, M.D (1984) Direct labelling of ipratropium bromide aerosol and its deposition pattern in normal subjects and patients with chronic bronchitis Thorax 39, 432-435
Sterk, P.J., Plomp, A., van de Vate, J.F and Quanjer, P H (1984) Physical properties of aerosols produced by several jet and ultrasonic nebulisers. Bull. Eur Physiopath. Respir. 20, 65-72
Taburet, A.M and Schmit, B. (1994) Pharmacokinetic optimisation of asthma treatment. Clin Pharmacokinet. 26, 396-418
Tesarowski, D.B., Viel, L., McDonell, W.N and Newhouse, M.T (1994) The rapid and effective administration of a β2-agonist to horses with heaves using a compact inhalation device and metered-dose inhalers Can vet. J 35, 170-173
Thomson, J R. and McPherson, E A (1981) Prophylactic effects of sodium cromoglycate on chronic obstructive pulmonary disease in the horse Equine vet. J. 13, 243-246
Votion, D., Ghafir, Y , Vandenput, S., Art, T and Lekeux P (1996a) Effects of romifidine on pulmonary function in horses with chronic obstructive pulmonary disease in clinical remission (part I) Réunion de la Société Belge de Physiologie et de Pharmacologie Fondamentales et Cliniques
Votion, D., Vandenput, S , Duvivier, D.H , Art, T and Lekeux P (1996b) Effects of romifidine on pulmonary function in horses with chronic obstructive pulmonary disease in acute crisis (part II) Réunion de la Société Belge de Physiologie et de Pharmacologie Fondamentales et Cliniques
Votion, D., Vandenput, S., Duvivier, D.H., Art, T and Lekeux, P. (1997) Analysis of equine scintigraphical lung images. The vet J. 153, 49-61.
Wang, Z.-W., Yu, M-F. and Robinson, N E (1995) Prejunctional muscarinic autoreceptors on horse airway cholinergic nerves. Life Sci. 56, 2255-2262
Yu, M., Robinson, N E., Wang, Z. and Derksen, F J (1992) Muscarinic receptor subtypes in equine tracheal smooth muscle Vet. Res. Commun 16, 301-310
Zainudin, B.M Z., Biddiscombe, M., Tolfree, S.E J , Short, M. and Spiro, S G. (1990) Comparison of bronchodilator responses and deposition patterns of salbutamol inhaled from a pressurised metered dose inhaler, as a dry powder, and as a nebulised solution Thorax 45, 469-473.