[en] A two-step "grafting from" method has been successfully carried out, which is based on the electrografting of polyacrylate chains containing an initiator for the atom transfer radical polymerization (ATRP) of 2-(tert-butylamino)ethyl methacrylate (TBAEMA) or copolymerization of TBAEMA with either monomethyl ether of poly(ethylene oxide) methacrylate (PEOMA) or acrylic acid (AA) or styrene. The chemisorption of this type of polymer brushes onto stainless steel surfaces has potential in orthopaedic surgery. These films have been characterized by ATR-FTIR, Raman spectroscopy, atomic force microscopy (AFM), and measurement of contact angles of water. The polymer formed in solution by ATRP and that one detached on purpose from the surface have been analyzed by size exclusion chromathography (SEC) and H-1 NMR spectroscopy. The strong adherence of the films onto stainless steel has been assessed by peeling tests. AFM analysis has shown that addition of hydrophilic comonomers to the grafted chains decreases the surface roughness. According to dynamic quartz crystal microbalance experiments, proteins (e.g., fibrinogen) are more effectively repelled whenever copolymer brushes contain neutral hydrophilic (PEOMA) co-units rather than negatively charged groups (PAA salt). Moreover, a 2- to 3-fold decrease in the fibrinogen adsorption is observed when TBAEMA is copolymerized with either PEOMA or AA rather than homopolymerized or copolymerized with styrene. Compared to the bare stainless steel surface, brushes of polyTBAEMA, poly (TBAEMA-co-PEOMA) and poly(TBAEMA-co-AA) decrease the bacteria adhesion by 3 to 4 orders of magnitude as revealed by Gram-positive bacteria S. aureus adhesion tests.
Research Center/Unit :
Center for Education and Research on Macromolecules (CERM)
Disciplines :
Materials science & engineering Chemistry
Author, co-author :
Ignatova, Miléna; Université de Liège - ULiège > Department of Chemistry > Center for Education and Research on Macromolecules (CERM)
Voccia, Samuel; Université de Liège - ULiège > Department of Chemistry > Center for Education and Research on Macromolecules (CERM)
Gilbert, Bernard ; Université de Liège - ULiège > Department of Chemistry > Laboratory of Analytical Chemistry and Electrochemistry
Markova, Nadya; Bulgarian Academy of Sciences, Sofia, Bulgaria > Institute of Microbiology
Cossement, Damien; University of Mons-Hainaut (UMH) > Service de Chimie Inorganique et Analytique (LASSIE)
Gouttebaron, Rachel; University of Mons-Hainaut (UMH) > Service de Chimie Inorganique et Analytique (LASSIE)
Jérôme, Robert ; Université de Liège - ULiège > Department of Chemistry > Center for Education and Research on Macromolecules (CERM)
Jérôme, Christine ; Université de Liège - ULiège > Department of Chemistry > Centre d'études et de rech. sur les macromolécules (CERM)
Language :
English
Title :
Combination of electrografting and atom-transfer radical polymerization for making the stainless steel surface antibacterial and protein antiadhesive
Publication date :
03 January 2006
Journal title :
Langmuir
ISSN :
0743-7463
eISSN :
1520-5827
Publisher :
Amer Chemical Soc, Washington, United States - Washington
BELSPO - SPP Politique scientifique - Service Public Fédéral de Programmation Politique scientifique F.R.S.-FNRS - Fonds de la Recherche Scientifique [BE] FRIA - Fonds pour la Formation à la Recherche dans l'Industrie et dans l'Agriculture [BE]
Gristina, A. G.; Naylor, P. T. Implant Associated Infection. In Biomaterials Science: An Introduction to Materials in Medicine; Ratner, B. D., Hoffman, A. S., Schoen, F. J., Lemons, J. E., Eds.; Academic Press: San Diego, CA, 1996; p 205.
Sofia, S. J.; Merrill, E. W. Protein adsorption on poly(ethylene glycol)-grafted silicon surfaces. In Poly(Ethylene Glycol): Chemistry and Biological Applications; Harris, J. M., Zalipsky, S., Eds.; American Chemical Society: Washington, DC, 1997; Chapter 22.
Gombotz, W. R.; Guanghui, W.; Horbett T. A.; Hoffman, A. S. Protein adsorption to and elution from polyether surfaces. In Poly(Ethylene Glycol) Chemistry Biotechnical and Biomedical Applications; Harris, J. M., Ed.; Plenum Press: New York, 1992; p 127.
Zhang, M.; Desai, T.; Ferrari, M. Biomaterials 1998, 19, 953.
McPherson, T.; Kidane, A.; Szleifer, I.; Park, K. Langmuir 1998, 14, 176.
Kiss, E.; Golander, C. G.; Eriksson, J. C. Prog. Colloid Polym. Sci. 1987, 74, 113.
Zou, X. P.; Kang, E. T.; Neoh, K. G. Plasmas Polym. 2002, 7, 151.
Gong, X.; Dai, L.; Griesser, H. J.; Mau, A. W. H. J. Polym. Sci., Part B: Polym. Phys. 2000, 38, 2323.
Mrksich, M.; Whitesides, G. M. Using self-assembled monolayers that present oligo(ethylene glycol) groups to control the interactions of proteins with surfaces. In Poly(Ethylene Glycol): Chemistry and Biological Applications; Harris, J. M., Zalipsky, S., Eds.; American Chemical Society: Washington, DC, 1997; Chapter 23.
Chapman, R. G.; Ostuni, E.; Liang, M. N.; Meluleni, G.; Kim, E.; Yan, L. ; Pier, G.; Warren, H. S.; Whitesides, G. M. Langmuir 2001, 17, 1225.
Prime, K. L.; Whitesides, G. M. J. Am. Chem. Soc. 1993, 115, 10714.
Prime, K. L.; Whitesides, G. M. Science 1991, 252, 1164.
Jo, S.; Park, K., Biomaterials 2000, 21, 605.
Kato, K.; Sano, S.; Ikada, Y. Colloids Surf. B 1995, 4, 221.
Ottersbach, P.; Inhester, M. PCT Int. Appl., 2003, WO 03/068316 A1.
Ottersbach, P.; Kossmann, B. PCT Int. Appl., 2003, WO 03/033033 A2.
Ottersbach, P.; Kossmann, B. PCT Int. Appl., 2002, WO 02/048070 A1.
Yuan, W.; Iroh, J. O. Trends Polym. Sci. 1993, 1, 388.
Baute, N.; Martinot, L.; Jérôme, R. J. Electroanal. Chem. 1999, 472, 83.
Baute, N.; Jérôme,C.; Martinot, L.; Mertens, M.; Geskin, V. M.; Lazzaroni, R.; Brédas, J. L.; Jérôme, R. Eur. J. Inorg. Chem. 2001, 5, 1097.
Voccia, S.; Jérôme, C.; Detrembleur, C.; Leclère, P. ; Gouttebaron, R.; Hecq, M.; Gilbert, B.; Lazzaroni, R.; Jérôme, R. Chem. Mater. 2003, 15, 923.
Claes, M.; Voccia, S.; Detrembleur, C.; Jérôme, C.; Gilbert, B.; Leclère, Ph.: Geskin, V. M.; Gouttebaron, R.; Hecq, M.; Lazzaroni, R.; Jérôme, R. Macromolecules 2003, 36, 5926.
Vangeyte, P.; Jérôme, R. J. Polym. Sci., Polym. Ed., Part A 2003, submitted.
Jérôme, C.; Gabriel, S.; Voccia, S.; Detrembleur, C.; Ignatova, M.; Gouttebaron, R.; Jérôme, R. Chem. Commun. 2003, 2500.
Tanaka, M.; Mochizuki, A.; Shiroya, T.; Motomura, T.; Shimura, K.; Onishi, M.; Okahata, Y. Colloids Surf. A: Physicochem. Eng. Aspects 2002, 203, 195.
Buttry, D. A.; Ward, M. D. Chem. Rev. 1992, 92, 1355.
Jérôme, R.; Mertens, M.; Martinot, L. Adv. Mater. 1995, 7, 807.
Mertens, M.; Calberg, C.; Martinot, L.; Jérôme. R. Macromolecules 1996, 29, 4910.
Simal, F.; Delaude, L.; Jan, D.; Demonceau, A.; Noels, A. F. Polym. Preprint 1999, 40, 336.
Simal, F.; Demonceau, A.; Noels, A. F. Tetrahedron Lett. 1999, 40, 5689.
Simal, F.; Demonceau, A.; Noels, A. F. Angew. Chem., Int. Ed. 1999, 38, 4.
An, Y. H.; Friedman, R. J. J. Biomed. Mater. Res. 1998, 43, 338.
Hendricks, S. K.; Kwok, C.; Shen, M.; Horbett, T. A.; Ratner, B. D.; Bryers, J. D. J. Biomed. Mater. Res. 2000, 50, 160.
Lee, J. H.; Kopecek, J.; Andrade, J. D. J. Biomed. Mater. Res. 1989, 23, 351.
Wan, S.; Mahmud, N.; Kushner, L.; Lindon, J. N.; Curme, J.; Merrill, E. W.; Salzman, E. W. Trans. Am. Soc. Artif. Intern. Organs 1982, 28, 482.
Coleman, D. L.; Gregonis, D. E.; Angrade, J. D. J. Biomed. Mater. Res. 1982, 16, 381.
Humphries, M.; Nemcek, J.; Cantwell, J. B.; Gerrard, J. J. FEMS Microbiol. Ecol. 1987, 45, 297.