Poster (Scientific congresses and symposiums)
Determination of the response factors of several desulfoglucosinolates used for quantitative analysis of Brassicaceae
Wathelet, Jean-Paul; Iori, R.; Mabon, N. et al.
2001GCIRC TEchnical meeting
 

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Keywords :
desulfoglucosinolate; Brassicacae; response factors
Abstract :
[en] Quantitative determination of total and individual glucosinolate contents in Brassica samples (rapeseed/canola, broccoli, cabbage, mustard…) is generally realised according to the reference method ISO 9167-1. After extraction, glucosinolates are purified, then desulfated by Helix pomatia sulfatase. The desulfo-glucosinolates (DSGSL) formed are then separated by reverse phase chromatography associated with UV detection (229 nm). As each compound can possess a very different molar extinction coefficient, it is important - on a quantitative point of view - to define accurately relative response factors. The response factors actually used in the official method have been determined experimentally through indirect methods and have been fixed, some years ago, by consensus between the various laboratories who took part in a ring test. They may need to be revised and calculated for a broader panel of glucosinolates.
Disciplines :
Biochemistry, biophysics & molecular biology
Author, co-author :
Wathelet, Jean-Paul ;  Université de Liège - ULiège > Gembloux Agro-Bio Tech
Iori, R.
Mabon, N.
Palmieri, S.
Leoni, O.
Rollin, P.
Marlier, M.
Language :
English
Title :
Determination of the response factors of several desulfoglucosinolates used for quantitative analysis of Brassicaceae
Publication date :
2001
Event name :
GCIRC TEchnical meeting
Event place :
Poznan, Poland
Available on ORBi :
since 01 March 2010

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