Reference : A HPTLC densitometric determination of flavonoids from Passiflora alata, P-edulis, P-...
Scientific journals : Article
Life sciences : Phytobiology (plant sciences, forestry, mycology...)
Human health sciences : Pharmacy, pharmacology & toxicology
A HPTLC densitometric determination of flavonoids from Passiflora alata, P-edulis, P-incarnata and P-caerulea and comparison with HPLC method
Pereira, C. A. M. [> > > >]
Yariwake, J. H. [> > > >]
Lancas, F. M. [> > > >]
Wauters, J. N. [> > > >]
Tits, Monique mailto [Université de Liège - ULiège > Département de pharmacie > Phytochimie et phytothérapie >]
Angenot, Luc mailto [Université de Liège - ULiège > Département de pharmacie > Pharmacognosie >]
Phytochemical Analysis
John Wiley & Sons Ltd
Yes (verified by ORBi)
[en] high-performance thin layer chromatography densitometry ; high-performance liquid chromatography ; flavonoids ; Passiflora
[en] A high-performance thin layer chromatographic (HPTLC) method was developed in order to determine quantitatively the flavonoids in leaves of Passiflora alata, P. edulis, P. caerulea and P. incarnata. The content of orientin and isoorientin was determined, and the results were compared with those obtained using a quantitative HPLC-UV method. The latter employed rutin as standard and was developed to analyse flavonoid content from Passiflora leaves for the purpose of ensuring the quality of Passiflora phytomedicines. The results obtained using the two methods indicate that there are qualitative and quantitative differences in the flavonoids of the reference Passiflora species studied. The two methods were also employed to analyse commercial samples to illustrate their application in qualitative ('fingerprint') and quantitative determination, demonstrating their feasibility in the quality control of flavonoids from crude Passiflora drugs and phytomedicines. The HPLC conditions used are also suitable for the quantitative analysis of aqueous extracts (Passiflora infusions). Copyright (C) 2004 John Wiley Sons, Ltd.

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