Aspergillus phoenicis; catalase; date flour; fungal morphology; mutant
Abstract :
[en] The production of extracellular catalase in date flour submerged medium by a selected mutant Aspergillus phoenicis K30 (member of the Aspergillus niger group) was investigated. The strain was tested in 500 ml shake-flasks and in a 20 l bioreactor with date powder as a single carbon source. Extracellular catalase production reached 59 U ml-1 in both cases. This value is much greater than that of a wild-type strain (9.5 U ml-1). Microscopic examination showed that the extracellular catalase production was correlated with the ramified hyphals morphology in the external layer of the pellets.
Agger T., Spohr AB., Carlsen M., Nielsen J. (1998). Growth and product formations of Aspergillus oryzae during submerged cultivations: verification of a morphologically structured model using fluorescent probes. Biotechnol. Bioeng. 57, p. 321–329.
Akertek D., Tarhan L. (1995). Characteristics of immobilised catalases and their applications in pasteurisation of milk. Appl. Biochem. Biotechnol. 50, p. 9555–9560.
Anonymous (2003). Données chiffrées N° 04, les palmiers dattiers en Algérie. Sous-direction des statistiques agricoles, Ministère de l’Agriculture et de Développement rural, Algeria.
Boulos L., Prevost M., Barbeau B., Coallier J., Desjardins R. (1999). Application of a new rapid staining method of direct enumeration of viable and total bacteria in drinking water. J. Microbiol. Methods 37, p. 77–86.
Caridis KA., Christakopulos P., Macris BJ. (1991). Simultaneous production of glucose oxidase and catalase by Alternaria alternata. Appl. Microb. Biotechnol. 34, p. 794–797.
El- Enshasy H., Hellmuth K., Rinas U. (1999). GpdAPromoter-Controlled production of glucose oxidase by recombinant. Aspergillus niger using nonglucose carbon sources. Appl. Biochem. Biotechnol. 81, p. 1–11.
Fiedurek J., Gromada A. (1997). Selection of biochemical mutants of Aspergillus niger with enhanced catalase production. Appl. Microbiol. Biotechnol. 47, p. 313–316.
Gregory EM., Fridovich I. (1974). Visualisation of catalase on acrylamide gels. Anal. Biochem. 58, p. 57–62.
Gromada A., Fiedurek J. (1997). Selective isolation of Aspergillus niger mutants with enhanced glucose oxidase production. J. Appl. Microbiol. 82, p. 648–652.
Halliwell B. (1990). How to characterise biological antioxidants. Free Radicals Res. Commun. 9, p. 1–32.
Nishikawa Y., Kawata Y., Nagai J. (1993). Effect of triton X-100 on catalase production by Aspergillus terreus IF06123. J. Ferment. Bioeng. 76, p. 235–236.
Nykanen M., Saarelainen R., Raudaskoski M., Nevalainen KMH., Mikkonen A. (1997). Expression and secretion of barley cysteine protease and peptidase β and cellobiohydrolase1 in Trichoderma reesei. Appl. Environ. Microbiol. 63, p. 4929–4937.
Petruccioli M., Fenice M., Piccioni P., Federici F. (1995). Effect of stirrer speed and buffering agents on the production of glucose oxidase and catalase by Penicillium variabile (P16) in benchtop bioreactor. Enzyme Microb. Technol. 17, p. 336–339.
Pluschkell S., Hellmuth K., Rinas U. (1996). Kinetic of glucose oxidase excretion by recombinant Aspergillus niger. Biotechnol. Bioeng. 51, p. 215–220.
Pontecorvo G., Roper JA., Himmons LM., MacDonald KD., Bufton AWJ. (1953). The genetic of Aspergillus nidulans. Adv. Genet. 5, p. 141–238.
Ryoo D., Choi CS. (1999). Surfaces thermodynamics of pellet formation in Aspergillus niger. Biotechnol. Lett. 21, p. 97–100.
Venkateshwaran G., Somashekar D., Prakash MH., Basappa SC., Richard J. (1999). Production and utilisation of catalase using Saccharomyces cerevisiae. Proc. Biochem. 34, p. 187–191.
Witteveen CFB., de Vandervoort PV., Swart K., Visser J. (1990). Glucose oxidase overproduction and negative mutants of Aspergillus niger. Appl. Microbiol. Biotechnol. 33, p. 683–686.
Wongwicharn A., McNeil B., Harvey LM. (1999). Effect of oxygen enrichment on morphology, growth and heterologous protein production in chemostat cultures of Aspergillus niger B1-D. Biotechnol. Bioeng. 65 (4), p. 416–424.
Wösten HAB., Moukha SM., Sietsma JH., Wessels JGH. (1991). Localisation of growth and secretion of proteins in Aspergillus niger. J. Gen. Microbiol. 137, p. 2017–2023.
