DNA methylation and cancer diagnosis: new methods and applications.

[en] Methylation of cytosines in cytosine-guanine (CpG) dinucleotides is one of the most important epigenetic alterations in animals. The presence of methylcytosine in the promoter of specific genes has profound consequences on local chromatin structure and on the regulation of gene expression. Changes in DNA methylation play a central role in carcinogenesis. Hypermethylation and consecutive transcriptional silencing of tumor-suppressor genes has been documented in numerous cancers. The identification of target genes silenced by this modification has a great impact on diagnosis, classification, definition of risk groups and prognosis of cancer patients. Here we outline genome-wide techniques aiming at the identification of relevant methylated promoters. Methods and applications allowing clinicians to monitor the methylation of target genes will be also reviewed.

Disciplines :

Oncology

Author, co-author :

Dehan, Pierre ; Université de Liège - ULiège > Département des sciences biomédicales et précliniques > Anatomie et cytologie pathologiques

Kustermans, Gaëlle ; Université de Liège - ULiège > Département des sciences biomédicales et précliniques > Anatomie et cytologie pathologiques

Guénin, Samuel ; Université de Liège - ULiège > Département des sciences biomédicales et précliniques > Anatomie et cytologie pathologiques

Horion, Julie ; Université de Liège - ULiège > Département des sciences biomédicales et précliniques > Anatomie et cytologie pathologiques

Boniver, Jacques ; Centre Hospitalier Universitaire de Liège - CHU > Anatomie pathologique

Delvenne, Philippe ; Université de Liège - ULiège > Département des sciences biomédicales et précliniques > Anatomie et cytologie pathologiques

Language :

English

Title :

DNA methylation and cancer diagnosis: new methods and applications.

Publication date :

2009

Journal title :

Expert Review of Molecular Diagnostics

ISSN :

1473-7159

eISSN :

1744-8352

Publisher :

Future Medicine Ltd

Volume :

Issue :

Pages :

651-7

Peer reviewed :

Peer Reviewed verified by ORBi

Available on ORBi :

since 26 February 2010

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