Interactions between fibroblasts and a reconstituted basement membrane matrix

[en] A gel-like reconstituted basement membrane matrix containing type IV collagen, laminin, entactin, nidogen, and heparan sulfate proteoglycan was used to examine the interactions between normal calf skin fibroblasts and basement membranes. Within 6 h after seeding, fibroblasts initiated a migration that resulted in the formation of a cellular network after 1 day of culture on top of the gel. Electron microscopy revealed that fibroblasts were able to remodel the basement membrane matrix by penetrating into the gel (from day 3), depositing fibronectin and collagen fibers, and retracting this extracellular matrix. Fibroblasts cultured on the Engelbreth-Holm-Swarm reconstituted basement membrane matrix displayed ultrastructural features characterized by a poor synthetic apparatus (rough endoplasmic reticulum and Golgi vesicles), a large cytoskeleton, and intracytoplasmic vesicles containing laminin. Thus the reconstituted basement membrane matrix is remodeled by skin fibroblasts, and reciprocally their ultrastructural morphologic features are affected by this matrix.

Disciplines :

Biochemistry, biophysics & molecular biology

Author, co-author :

Emonard, H.

Calle, A.

Grimaud, J. A.

Peyrol, S.

Castronovo, Vincenzo ; Université de Liège - ULiège > Département des sciences biomédicales et précliniques > Biologie générale et cellulaire - GIGA-R : Labo de recherche sur les métastases

Noël, Agnès ; Université de Liège - ULiège > Département des sciences biomédicales et précliniques > Biologie cellulaire et moléculaire appliquée à l'homme

Lapière, Charles

Kleinman, H. K.

Foidart, Jean-Michel ; Université de Liège - ULiège > Département des sciences cliniques > Gynécologie - Obstétrique - Labo de biologie des tumeurs et du développement

Language :

English

Title :

Interactions between fibroblasts and a reconstituted basement membrane matrix

Publication date :

1987

Journal title :

Journal of Investigative Dermatology

ISSN :

0022-202X

eISSN :

1523-1747

Publisher :

Nature Publishing Group, New York, United States - New York

Volume :

Pages :

156-163

Peer reviewed :

Peer Reviewed verified by ORBi

Available on ORBi :

since 26 May 2010

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Bibliography

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