[en] Extracts of wheat etioplast membranes obtained after treatment with 7 mM n-octyl-beta-D-glucopyranoside (OG), n-dodecyl-beta-D-maltoside (DM) or Triton X-100 contained the three spectral forms of Pchlide (the photoactive Pchlide(638) and Pchlide(638) and the inactive Pchlide(650)) in various relative amounds. The OG extract had a Pchlide composition close to that of the intact membranes whereas the DM extract was enriched in Pchlide(638) and the Triton extract was enriched in Pchlide(630). Measurements of the kinetics of phototransformation and of time-resolved absorbance spectra during phototransformation in continuous Light shows that the inactive Pchlide(630) is in fact slowly transformed to Chlide, especially in the Triton extract where this form is more abundant. Addition of NADPH favours the phototransformation of Pchlide(630) and the slow regeneration of Pchlide(638) and Pchlide(650) from Pchlide(630) in darkness after illumination. No such regeneration was however observed in the Triton extract. NADPH had only slight effects on the Chlide shift towards shorter wavelengths after phototransformation in solubilized membranes.
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