Article (Scientific journals)
Arrest of MCF-7 cell migration by laminin in vitro: possible mechanisms.
Coopman, P.; Verhasselt, B.; Bracke, M. et al.
1991In Clinical and Experimental Metastasis, 9 (5), p. 469-84
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Keywords :
Adenocarcinoma/chemistry/physiopathology; Breast Neoplasms/chemistry/physiopathology; Cell Movement/drug effects; Culture Media; Humans; Laminin/pharmacology; Receptors, Antigen/analysis; Receptors, Immunologic/analysis/metabolism; Receptors, Laminin; Tumor Cells, Cultured/drug effects
Abstract :
[en] Laminin, a major basement membrane component, arrested the migration of MCF-7/AZ human breast adenocarcinoma cells that were not invasive in vitro. Migration of invasive MCF-7/6 cells was not affected by laminin. Both cell types expressed the 67 kD laminin receptor, at both mRNA and protein level, but did not express the alpha 6 subunit of the VLA-6 integrin-type laminin receptor. The presence of YIGSR peptides (100 micrograms/ml), reported to block the interaction between laminin and its 67 kD receptor, did not change the migratory response of MCF-7/AZ or MCF-7/6 cells when meeting laminin lanes. In addition, the migration of these cell types was not affected by the presence of 17-beta-estradiol (10(-6) M) or all-trans retinoic acid (10(-6) M), which were both reported to increase the number of 67 kD receptors. We could therefore not assign an involvement of the 67 kD receptors in migration of MCF-7 cells on laminin, nor did we find evidence that conditioned medium of MCF-7/6 cells contains factors that are able to initiate migration of MCF-7/AZ cells on laminin.
Disciplines :
Biochemistry, biophysics & molecular biology
Author, co-author :
Coopman, P.
Verhasselt, B.
Bracke, M.
De Bruyne, G.
Castronovo, Vincenzo ;  Université de Liège - ULiège > Département des sciences biomédicales et précliniques > Biologie générale et cellulaire - GIGA-R : Labo de recherche sur les métastases
Sobel, M.
Foidart, Jean-Michel ;  Université de Liège - ULiège > Département des sciences cliniques > Gynécologie - Obstétrique - Labo de biologie des tumeurs et du développement
Van Roy, F.
Mareel, M.
Language :
English
Title :
Arrest of MCF-7 cell migration by laminin in vitro: possible mechanisms.
Publication date :
1991
Journal title :
Clinical and Experimental Metastasis
ISSN :
0262-0898
Publisher :
Kluwer Academic Publishers, Dordrecht, Netherlands
Volume :
9
Issue :
5
Pages :
469-84
Peer reviewed :
Peer Reviewed verified by ORBi
Funders :
Belgisch Werk tegen Kanker
ASLK Kankerfonds
F.R.S.-FNRS - Fonds de la Recherche Scientifique [BE]
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