[en] The cytotoxicity and the selective antiprotozoal activity of some Strychnos alkaloids, namely strychnopentamine (SP) and usambarensine (US) (7) led us to analyze and compare their effects with emetine (EM) by using mouse B16 melanoma cells cultivated in vitro. We observed by cytological analysis and proliferation rate studies that these substances induce analogous cytotoxic effects in B16 cells, but at different concentrations i.e. formation of lamellar bodies in the cytoplasm, the which contain pre-melanosomes in the case of SP and US, vacuoles and blebs. At concentrations near their respective IC50, SP and US, but not EM, decreased colony formation. We showed by incorporation of labelled precursors that SP and US first inhibit RNA synthesis while EM initially acts on protein synthesis. These alkaloids increased melanin synthesis. Furthermore, only EM and SP caused hemolysis of sheep red blood corpuscles. This could explain why the rate of antiplasmodial activity is higher for SP and EM.
Disciplines :
Pharmacie, pharmacologie & toxicologie
Auteur, co-auteur :
Bonjean, Karine ; Université de Liège - ULiège > Département des sciences précliniques MI > Histologie - Cytologie
De Pauw-Gillet, Marie-Claire ; Université de Liège - ULiège > Département des sciences biomédicales et précliniques > Histologie - Cytologie - Département des sciences biomédicales et précliniques
Quetin-Leclercq, Joëlle
Angenot, Luc ; Université de Liège - ULiège > Département de pharmacie > Pharmacognosie
Bassleer, Roger ; Université de Liège - ULiège > Relations académiques et scientifiques (Médecine)
Langue du document :
Anglais
Titre :
In Vitro Cytotoxic Activity of Two Potential Anticancer Drugs Isolated from Strychnos: Strychnopentamine and Usambarensine
Date de publication/diffusion :
1996
Titre du périodique :
Anticancer Research
ISSN :
0250-7005
eISSN :
1791-7530
Maison d'édition :
International Institute of Anticancer Research, Grèce
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