[en] In this work, we have analysed the effects of alpha-hederin, a monodesmosidic triterpenoid saponin isolated from Hedera helix, on mouse B16 melanoma cells and non-cancer mouse 3T3 fibroblasts cultured in vitro. Our results indicate that, in a serum-free medium, alpha-hederin is cytotoxic and inhibits proliferation in both cell lines at rather low concentrations (< 5 micrograms/ml) after only 8 hours of treatment. Its cytotoxicity decreases in the presence of serum in which BSA seems to be able to bind the saponin. alpha-Hederin also induces vacuolization of the cytoplasm and membrane alterations leading to cell death.
Disciplines :
Pharmacy, pharmacology & toxicology
Author, co-author :
Danloy, S.
Quetin-Leclercq, J.
Coucke, P.
De Pauw-Gillet, Marie-Claire ; Université de Liège - ULiège > Département des sciences biomédicales et précliniques > Histologie - Cytologie - Département des sciences biomédicales et précliniques
Elias, R.
Balansard, G.
Angenot, Luc ; Université de Liège - ULiège > Département de pharmacie > Pharmacognosie
Bassleer, Roger ; Université de Liège - ULiège > Relations académiques et scientifiques (Médecine)
Language :
English
Title :
Effects of Alpha-Hederin, a Saponin Extracted from Hedera Helix, on Cells Cultured in Vitro