Article (Scientific journals)
Bifidobacteria as indicators of faecal contamination along a sheep meat production chain
Delcenserie, Véronique; Loncaric, D.; Bonaparte, Christine et al.
2008In Journal of Applied Microbiology, 104 (1), p. 276-284
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Keywords :
Bifidobacterium choerinum; Bifidobacterium pseudolongum; Bifidobacterium thermophilum; Chaperonin 60/genetics; Colony Count, Microbial; DNA-DNA hybridization; Escherichia coli/isolation & purification; faecal indicators; hsp60 gene; meat; mupirocin; PCR; sheep; slaughter
Abstract :
[en] Aims: The potential use of bifidobacteria as indicators for faecal contamination was studied along a sheep meat production and processing chain. The levels of bifidobacteria were compared with those of Escherichia coli. Total viable counts were followed along the chain (244 samples). Methods and Results: Forty-three per cent of the samples contained bifidobacteria, of which 15% were solely detected using a PCR method based on the hsp60 gene and not by a culture-based method. Bifidobacteria were detected in only three of nine sheep faeces samples using one or the other method. However, carcasses (types C and E) were highly contaminated. These sample types (30% and 28%, respectively) were positive for bifidobacteria and negative for E. coli. The species Bifidobacterium pseudolongum and Bif. thermophilum, isolated from faecal samples, were predominant. Bifidobacterium choerinum were found in C, D, E and F sample types. Conclusions: Bifidobacteria were shown more efficient than E. coli in carcasses samples. The presence of Bif. choerinum suggested a faecal pork contamination. Significance and Impact of the Study: Detection and identification of bifidobacteria, in correlation with E. coli counting, should improve hygiene quality of mutton processing chains.
Disciplines :
Food science
Microbiology
Author, co-author :
Delcenserie, Véronique ;  Université de Liège - ULiège > Département de sciences des denrées alimentaires > Gestion de la qualité dans la chaîne alimentaire
Loncaric, D.;  University of Veterinary Medicine Vienna > Meat Technology and Food Science > Institute of Meat Hygiene
Bonaparte, Christine;  University of Veterinary Medicine Vienna, > Meat Technology and Food Science, > Institute for Meat Hygiene
Upmann, Mathias;  University of Veterinary Medicine Vienna > Meat Technology and Food Science > Institute of Meat Hygiene
China, Bernard;  Université de Liège - ULiège - ULG > Sciences des Denrées alimentaires > Microbiologie des denrées alimentaires
Daube, Georges  ;  Université de Liège - ULiège > Sciences des Denrées alimentaires > Microbiologie des denrées alimentaires
Gavini, Françoise;  Institut Scientifique de Recherche Agronomique - INRA > Microbiologie et Chaîne Alimentaire, Villeneuve d'Ascq
Language :
English
Title :
Bifidobacteria as indicators of faecal contamination along a sheep meat production chain
Publication date :
2008
Journal title :
Journal of Applied Microbiology
ISSN :
1364-5072
eISSN :
1365-2672
Publisher :
Blackwell Publishing, Oxford, United Kingdom
Volume :
104
Issue :
1
Pages :
276-284
Peer reviewed :
Peer Reviewed verified by ORBi
Name of the research project :
BIFID
Funders :
DG RDT - Commission Européenne. Direction Générale de la Recherche et de l'Innovation [BE]
Available on ORBi :
since 30 July 2008

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