[en] Aims: The potential use of bifidobacteria as indicators for faecal contamination was studied along a sheep meat production and processing chain. The levels of bifidobacteria were compared with those of Escherichia coli. Total viable counts were followed along the chain (244 samples). Methods and Results: Forty-three per cent of the samples contained bifidobacteria, of which 15% were solely detected using a PCR method based on the hsp60 gene and not by a culture-based method. Bifidobacteria were detected in only three of nine sheep faeces samples using one or the other method. However, carcasses (types C and E) were highly contaminated. These sample types (30% and 28%, respectively) were positive for bifidobacteria and negative for E. coli. The species Bifidobacterium pseudolongum and Bif. thermophilum, isolated from faecal samples, were predominant. Bifidobacterium choerinum were found in C, D, E and F sample types. Conclusions: Bifidobacteria were shown more efficient than E. coli in carcasses samples. The presence of Bif. choerinum suggested a faecal pork contamination. Significance and Impact of the Study: Detection and identification of bifidobacteria, in correlation with E. coli counting, should improve hygiene quality of mutton processing chains.
Disciplines :
Food science Microbiology
Author, co-author :
Delcenserie, Véronique ; Université de Liège - ULiège > Département de sciences des denrées alimentaires > Gestion de la qualité dans la chaîne alimentaire
Loncaric, D.; University of Veterinary Medicine Vienna > Meat Technology and Food Science > Institute of Meat Hygiene
Bonaparte, Christine; University of Veterinary Medicine Vienna, > Meat Technology and Food Science, > Institute for Meat Hygiene
Upmann, Mathias; University of Veterinary Medicine Vienna > Meat Technology and Food Science > Institute of Meat Hygiene
China, Bernard; Université de Liège - ULiège - ULG > Sciences des Denrées alimentaires > Microbiologie des denrées alimentaires
Daube, Georges ; Université de Liège - ULiège > Sciences des Denrées alimentaires > Microbiologie des denrées alimentaires
Gavini, Françoise; Institut Scientifique de Recherche Agronomique - INRA > Microbiologie et Chaîne Alimentaire, Villeneuve d'Ascq
Language :
English
Title :
Bifidobacteria as indicators of faecal contamination along a sheep meat production chain
Publication date :
2008
Journal title :
Journal of Applied Microbiology
ISSN :
1364-5072
eISSN :
1365-2672
Publisher :
Blackwell Publishing, Oxford, United Kingdom
Volume :
104
Issue :
1
Pages :
276-284
Peer reviewed :
Peer Reviewed verified by ORBi
Name of the research project :
BIFID
Funders :
DG RDT - Commission Européenne. Direction Générale de la Recherche et de l'Innovation
Ashby, K.D., Wen, J., Chowdhury, P., Casey, T.A., Rasmussen, M.A. Petrich, J.W. (2003) Fluorescence of dietary porphyrins as a basis for real-time detection of fecal contamination on meat. J Agric Food Chem 51, 3502 3507.
Aslam, M., Nattress, F., Greer, G., Yost, C., Gill, C. McMullen, L. (2003) Origin of contamination and genetic diversity of Escherichia coli in beef cattle. Appl Environ Microbiol 69, 2794 2799.
Beerens, H. (1998) Bifidobacteria as indicators of faecal contamination in meat and meat products: detection, determination of origin and comparison with Escherichia coli. Int J Food Microbiol 40, 203 207.
Beerens, H., Hass Brac de la Perriere, B. Gavini, F. (2000) Evaluation of the hygienic quality of raw milk based on the presence of bifidobacteria: the cow as a source of faecal contamination. Int J Food Microbiol 54, 163 169.
Biss, M.E. Hathaway, S.C. (1996) Microbiological contamination of ovine carcasses associated with the presence of wool and fecal material. J Appl Bacteriol 81, 594 600.
Brown, M.H., Gill, C.O., Hollingsworth, J., Nickelson, R. II., Seward, S., Sheridan, J.J., Stevenson, T., Sumner, J.L. et al. (2000) The role of microbiological testing in systems for assuring the safety of beef. Int J Food Microbiol 62, 7 16.
Calicioglu, M., Buege, D.R., Ingham, S.C. Luchansky, J.B. (1999) Recovery of Escherichia coli Biotype I and Enterococcus spp. during refrigerated storage of beef carcasses inoculated with a fecal slurry. J Food Prot 62, 944 947.
De Ley, J., Cattoir, H. Reynaerts, A. (1970) The quantitative measurement of DNA hybridization from renaturation rates. Eur J Biochem 12, 133 142.
Delcenserie, V., Bechoux, N., China, B., Daube, G. Gavini, F. (2005) A PCR method for detection of bifidobacteria in raw milk and raw milk cheese: comparison with culture-based methods. J Microbiol Methods 61, 55 67.
DIN 10161-2 (1984) Microbiological examination of meat and meat products; determination of the aerobic viable count at 30°C, drop plating method. Berlin : Beuth.
Ellis, D.I., Broadhurst, D., Kell, D.B., Rowland, J.J. Goodacre, R. (2002) Rapid and quantitative detection of the microbial spoilage of meat by Fourier transform infrared spectroscopy and machine learning. Appl Environ Microbiol 68, 2822 2828.
Gavini, F. Beerens, H. (1999) Origin and identification of bifidobacteria strains isolated from meat and meat products. Int J Food Microbiol 46, 81 85.
Gavini, F., Pourcher, A.M., Neut, C., Monget, D., Romond, C., Oger, C. Izard, D. (1991) Phenotypic differentiation of bifidobacteria of human and animal origins. Int J Syst Bacteriol 41, 548 557.
Gavini, F., Delcenserie, V., Kopeinig, K., Pollinger, S., Beerens, H., Bonaparte, C. Upmann, M. (2006) Bifidobacterium species isolated from animal feces and from beef and pork meat. J Food Prot 69, 871 877.
Gill, C.O., Baker, L.P. Jones, T. (1999) Identification of inadequately cleaned equipment used in a sheep carcass-breaking process. J Food Prot 62, 637 643.
Gilpin, B., James, T., Nourozi, F., Saunders, D., Scholes, P. Savill, M. (2003) The use of chemical and molecular microbial indicators for faecal source identification. Water Sci Technol 47, 39 43.
Hsu, F.C., Shieh, Y.S. Sobsey, M.D. (2002) Enteric bacteriophages as potential fecal indicators in ground beef and poultry meat. J Food Prot 65, 93 99.
Johnson, L.K., Brown, M.B., Carruthers, E.A., Ferguson, J.A., Dombek, P.E. Sadowsky, M.J. (2004) Sample size, library composition, and genotypic diversity among natural populations of Escherichia coli from different animals influence accuracy of determining sources of fecal pollution. Appl Environ Microbiol 70, 4478 4485.
Kodikara, C.P., Crew, H.H. Stewart, G.S. (1991) Near on-line detection of enteric bacteria using lux recombinant bacteriophage. FEMS Microbiol Lett 67, 261 265.
Lynch, P.A., Gilpin, B.J., Sinton, L.W. Savill, M.G. (2002) The detection of Bifidobacterium adolescentis by colony hybridization as an indicator of human faecal pollution. J Appl Microbiol 92, 526 533.
Maciorowski, K.G., Pillai, S.D. Ricke, S.C. (2001) Presence of bacteriophages in animal feed as indicators of fecal contamination. J Environ Sci Health B 36, 699 708.
Marmur, J. (1961) A procedure for isolation of deoxyribonucleic acid from microorganisms. J Mol Biol 3, 208 218.
Matsuki, T., Watanabe, K., Tanaka, R. Oyaizu, H. (1998) Rapid identification of human intestinal bifidobacteria by 16S rRNA-targeted species- and group-specific primers. FEMS Microbiol Lett 167, 113 121.
Matsuki, T., Watanabe, K., Tanaka, R., Fukuda, M. Oyaizu, H. (1999) Distribution of bifidobacterial species in human intestinal microflora examined with 16S rRNA-gene-targeted species-specific primers. Appl Environ Microbiol 65, 4506 4512.
Mossel, D.A.A. (1958) The suitability of bifidobacteria as part of a more extended bacterial association indicating faecal contamination of foods. In 7th International Congress of Microbiology Abstracts of Papers, pp. 440 441. Uppsala, Sweden : Almquist & Wikesells.
Nebra, Y., Bonjoch, X. Blanch, A.R. (2003) Use of Bifidobacterium dentium as an indicator of the origin of fecal water pollution. Appl Environ Microbiol 69, 2651 2656.
Radstrom, P., Knutsson, R., Wolffs, P., Lovenklev, M. Lofstrom, C. (2004) Pre-PCR processing: strategies to generate PCR-compatible samples. Mol Biotechnol 26, 133 146.
Scardovi, V. (1986) Irregular nonsporing gram-negative rods. Genus Bifidobacterium. Orla-jensen 1924. In Bergey's Manual of Systematic Bacteriology ed. Sneath, P.H.A., Mair, N.S., Shorpe, M.E. Holt, J.Q. pp. 1418 1434. Baltimore : Williams and Wilkins Co.
Scardovi, V., Trovatelli, L.D., Biavati, B. Zani, G. (1979) Bifidobacterium cuniculi, Bifidobacterium choerinum, Bifidobacterium boum and Bifidobacterium pseudocatenulatum: four new species and their deoxyribonucleic acid homology relationships. Int J Syst Bacteriol 29, 291 311.
Sneath, P.H.A. Sokal, R.R. (1973) Numerical taxonomy: the principles and practice of numerical classification. San Francisco, CA : W. H. Freeman.
Sumner, J., Petrenas, E., Dean, P., Dowsett, P., West, G., Wiering, R. Raven, G. (2003) Microbial contamination on beef and sheep carcasses in South Australia. Int J Food Microbiol 81, 255 260.
Tannock, G.W. (2001) Molecular assessment of intestinal microflora. Am J Clin Nutr 73, 410S 414S.
Thornton, C.G. Passen, S. (2004) Inhibition of PCR amplification by phytic acid, and treatment of bovine fecal specimens with phytase to reduce inhibition. J Microbiol Methods 59, 43 52.
Upmann, M., Jakob, P. Reuter, G. (2000) Microbial transfer during cutting and deboning of pork in a small-scale meat processing plant. Dairy Food Environ Sanitation 20, 14 23.
Venieri, D., Vantarakis, A., Komninou, G. Papapetropoulou, M. (2004) Differentiation of faecal Escherichia coli from human and animal sources by random amplified polymorphic DNA-PCR (RAPD-PCR). Water Sci Technol 50, 193 198.
Warriner, K., Aldsworth, T.G., Kaur, S. Dodd, C.E. (2002) Cross-contamination of carcasses and equipment during pork processing. J Appl Microbiol 93, 169 177.
Wehausen, J.D., Ramey, R.R. II. Epps, C.W. (2004) Experiments in DNA extraction and PCR amplification from bighorn sheep feces: the importance of DNA extraction method. J Hered 95, 503 509.
Zweifel, C. Stephan, R. (2003) Microbiological monitoring of sheep carcass contamination in three Swiss abattoirs. J Food Prot 66, 946 952.
