Pangasianodon hypophthalmus; Real time PCR; Trichlorfon; Heat shock protein 70; Gene expression; Cytochrome oxidase subunit 1; Acetylcholinesterase; Aquaculture; Biomarkers
Abstract :
[en] Trichlorfon (TRC) is the most common organophosphorous insecticide used in aquaculture practices in Southeast Asian countries. Indiscriminate use of TRC can either damage or alter the enzymatic and hormonal activities in the living organisms. In this present study, therefore, toxicogenomic analyses using real time PCR was used to characterize expression levels of various genes in Pangasianodon hypophthalmus after exposure to three concentrations, the 96 h 1/100LC50 (0.01 mg/L), the 96 h 1⁄10LC50 (0.1 mg/L) and the 96 h 1⁄2LC50 (0.5 mg/L) of TRC for 6 h, 24 h, 96 h, 7 days, 14 days, 28 days and 56 days respectively. The expression kinetics of stress and other cellular toxicity representative genes such as heat shock protein70 (HSP70), growth hormone, acetylcholinesterase (AChE), trypsinogen, cytochrome P4501B (CYP1B) and cytochrome oxidase subunit 1 (COI) were investigated in liver and gills. TRC at a level of 0.1 mg/L and 0.5 mg/L induced a time and dose-dependent increase in the expression of the HSP70, COI and CYPIB while the transcript level of AChE, growth hormone and trypsinogen were significantly down-regulated. These results could permit to develop a ‘molecular biomarker system’ which can be applied as a first-tier method of identifying contaminant exposure before effects at population level occur.
Disciplines :
Biochemistry, biophysics & molecular biology
Author, co-author :
Sinha, Amit Kumar
Vanparys, Caroline
De Boeck
Kestemont
Wang, Neil
Phuong, Nguyen Thanh
Scippo, Marie-Louise ; Université de Liège - ULiège > Département de sciences des denrées alimentaires > CART, Analyse des denrées alimentaires
De Coen, Wim
Robbens, Johan
Language :
English
Title :
Expression characteristics of potential biomarker genes in Tra catfish, Pangasianodon hypophthalmus, exposed to trichlorfon
Publication date :
2010
Journal title :
Comparative Biochemistry and Physiology. Part D, Genomics and Proteomics
M. Achard-Joris, P. Gonzalez, V. Marie, M. Baudrimont, and J.P. Bourdineaud Cytochrome c oxydase subunit I gene is up-regulated by cadmium in freshwater and marine bivalves Biometals 19 2006 237 244
N. Basu, A.E. Todgham, P.A. Ackerman, M.R. Bibeau, K. Nakano, P.M. Schulte, and G.K. Iwama Heat Shock Protein genes and their functional significance in fish Gene 295 2002 173 183
G.W. Benz, R.L. Otting, and A. Case Redescription of Argulus melanosticus (Branchiura : Argulidae), a parasite of California grunion (Leuresthes tenuis: Atherinidae), with notes regarding chemical control of A. melanosticus in a captive host population J. Parasitol. 81 1995 754 761
G. Benzi, O. Pastoris, R.F. Marzatico, R.F. Villa, and D. Curti The mitochondrial electron transfer alteration as a factor involved in the aging brain Neurobiol. Aging 13 1992 361 368
G. Bocquené, F. Galgani, and P. Truquet Characterization and assay conditions for use of AChE activity from several marine species in pollution monitoring Mar. Environ. Res. 30 1990 75 89
G. Bocquené, C. Bellanger, Y. Cadiou, and F. Galgani Joint action of combinations of pollutants on the acetylcholinesterase activity of several marine species Ecotoxicology 4 1995 226 279
C.J. Bolter, and W. Chefurka Extramitochondrial release of hydrogen peroxide from insect and mouse liver mitochondria using the respiratory inhibitors phosphine, myxothiazol, and antimycin and spectral analysis of inhibited cytochromes Arch. Biochem. Biophys. 278 1990 65 72
J.S. Boone, and J.E. Chambers Biochemical factors contributing to toxicity differences among chlorpyrifos, parathion, and methyl parathion in mosquitofish (Gambusia affinis) Aquat. Toxicol. 39 1997 333 343
J.T.M. Buters, S. Sakai, T. Richter, T. Pineau, D.L. Alexander, U. Savas, J. Doehmer, J.M. Ward, C.R. Jefcoate, and F.J. Gonzalez Cytochrome P450 CYP1B1 determines susceptibility to 7, 12-dimethylbenz[α]anthracene-induced lymphomas Proc. Natl. Acad. Sci. USA 96 1999 1977 1982
R.A. Capaldi Structure and assembly of Cytochrome c oxidase Arch. Biochem. Biophys. 280 1990 252 262
M.P. Carajaville, M.J. Bebianno, J. Blasco, C. Porte, C. Sarasquete, and A. Viarengo The use of biomarkers to assess the impact of pollution in coastal environment of the Iberian Peninsula: a practical approach Sci. Total Environ. 247 2000 295 311
C. Chang, P. Lee, C.H. Liu, and W. Cheng Trichlorfon, an organophosphorus insecticide, depresses the immune responses and resistance to Lactococcus garvieae of the giant freshwater prawn Macrobrachium rosenbergii Fish Shellfish Immunol. 20 2006 574 585
N. Chevre, F. Gagne, P. Gagnon, and C. Blaise Application of rough sites analysis to identify polluted aquatic sites based on a battery of biomarkers: a comparison with classical methods Chemosphere 51 2003 13 23
G. De Boeck, B. De Wachter, A. Vlaeminck, and R. Blust Effect of cortisol treatment and/or sublethal copper exposure on copper uptake and heat shock protein levels in common carp, Cyprinus carpio Environ. Toxicol. Chem. 22 2003 1122 1126
F.R. de la Torre, L. Ferrari, and A. SalibiaÍn Biomarkers of a native fish species (Cnesterodon decemmaculatus) application to the water toxicity assessment of a peri-urban polluted river of Argentina Chemosphere 59 2005 577 583
M.A.H. El-Kady, R. Mitsuo, Y. Kaminishi, and T. Itakura cDNA cloning, sequences analysis and expression of 3-methylcholanthrene- inducible cytochrome P450 1B1 in carp (Cyprinus carpio) Environ. Sci. 11 2004 231 240
M.A.H. El-Kady, R. Mitsuo, Y. Kaminishi, and T. Itakura Isolation of cDNA of novel cytochrome P450 1B2 gene from carp (Cyprinus carpio) and induced expression in gills Environ. Sci. 11 2004 345 354
M. Eto Organophosphorous Pesticides: Organic and Biological Chemistry 1974 CRC Press Boca Raton, FL
F. Galgani, and G. Bocquené In vitro inhibition of acetylcholinesterase from four marine species by organophosphates and carbamates Bull. Environ. Contam. Toxicol. 45 1990 243 249
R. Garcia-Repetto, D. Martinez, and M. Repetto Malathion and dichlorvos toxicokinetics after the oral administration of malathion and trichlorfon Vet. Hum. Toxicol. 37 1995 306 309
C.A.J. Godard, M.J. Leaver, M.R. Said, R.L. Dickerson, S. George, and J.J. Stegeman Identification of cytochrome P450 1B-like sequences in two teleost fish species (scup, Stenotomus chrysops and plaice, Pleuronectes platessa) and in a Cetacean (striped dolphin, Stenella coeruleoalba) Mar. Environ. Res. 50 2000 7 10
P. Gonzalez, Y. Dominique, J.-C. Massabuau, A. Boudou, and J.-P. Bourdineaud Comparative effects of dietary methylmercury on gene expression in liver, skeletal muscle, and brain of the zebra fish (Danio rerio) Environ. Sci. Technol. 39 2005 3972 3980
K. Grave, M. Engelstad, and N.E. Soli Utilization of dichlorvos and trichlorfon in salmonid farming in Norway during 1981-1988 Acta Vet. Scand. 32 1991 1 7
A.T.B. Guimaraes, and P. Calil Growth evaluation of Oreochromis niloticus (Cichlidae, Neopterygii) exposed to trichlorfon Braz. Arch. Biol. Technol. 51 2008 323 332
A.T.B. Guimaraes, H.C. Silva de Assis, and W. Boeger The effect of trichlorfon on acetylcholinesterase activity and histopathology of cultivated fish Oreochromis niloticus Ecotoxicol. Environ. Saf. 68 2007 57 62
D.Q. Hai, S.I. Varga, and B. Matkovics Organophosphate effects on antioxidant system of carp (Cyprinus carpio) and catfish (Ictalurus nebulosus) Comp. Biochem. Physiol. C 117 1997 83 88
A.V. Hallare, H.R. Kohler, and R. Triebskorn Development toxicity and stress protein responses in zebrafish embryos after exposure to diclofenac and its solvents, DMSO Chemosphere 56 2004 659 666
F.U. Hartl Molecular chaperones in cellular protein folding Nature 381 1996 571 580
H.M.A. Hassanein, M.A. Banhawy, F.M. Soliman, S.A. Abdel-Rehim, W.E.G. Muller, and H.C. Schroder Induction of Hsp70 by the herbicide oxyfluorfen (Goal) in the Egyptian Nile Fish, Oreochromis niloticus Arch. Environ. Contam. Toxicol. 37 1999 78 84
N. Herwig Handbook of Drugs and Chemicals Used in Treatment of Fish Diseases, A Manual of Fish Pharmacology and Material Medica 1979 Thomas Publishers Springfield, IL, USA
W. Hofer Chemistry of metrifonate and dichlorvos Acta Pharmacol. Toxicol. 49 1981 7 14
G.E. Howe, L.L. Marking, T.D. Bills, J.J. Rach, and F.L. Mayer Effects of water temperature and pH on toxicity of terbufos, Trichlorfon, 4- nitrophenol and 2, 4-dinitrophenol to the amphipod Gammarus pseudolimnaeus and rainbow trout (Oncorhynchus mykiss) Environ. Toxicol. Chem. 13 1994 51 66
M.J. Leaver, and S.G. George A cytochrome P4501B gene from a fish, Pleuronectes platessa Gene 256 2000 83 91
S.M. Lee, S.B. Lee, C.H. Park, and J. Choi Expression of heat shock protein and hemoglobin genes in Chironomus tentans (Diptera, chironomidae) larvae exposed to various environmental pollutants: a potential biomarker of freshwater monitoring Chemosphere 65 2006 1074 1081
G.M. Ludwig Effects of Trichlorfon, fenthion, and diflubenzuron on the zooplankton community and on production of reciprocal-cross hybrid striped bass fry in culture ponds Aquaculture 110 1993 301 319
P. Magni, G. De Falco, C. Falugi, M. Franzoni, M. Monteverde, E. Perrone, M. Sgro, and C. Bolognesi Genotoxicity biomarkers and acetylcholinesterase activity in natural populations of Mytilus galloprovincialis along a pollution gradient in the Gulf of Oristano (Sardinia, western Mediterranean) Environ. Pollut. 142 2006 65 72
I. Marigomez, M. Soto, M.P. Cajaraville, E. Angulo, and L. Giamberini Cellular and subcellular distribution of metals in molluscs Microsc. Res. Tech. 56 2002 358 392
J.L. Messenger, and F. Esnault Traitement par le dichlorvos des copepodoses de la truite arc-en-ciel elevée en mer: modalities de traitement adaptées aux conditions environmentales françaises C. Michel, D.J. Alderman, Chemotherapy in Aquaculture: From Theory to Reality 1992 Office International des Epizootics Paris 195 205
A. Mohammad, A. Ranjbar, S. Shahin, S. Nikfar, and A. Rezaie Pesticides and oxidative stress: a review Med. Sci. Monit. 10 2004 141 147
D.A. Monteiro, J.A. Almeida, F.T. Rantin, and A.L. Kalinin Oxidative stress biomarkers in the freshwater characid fish, Brycon cephalus, exposed to organophosphorus insecticide Folisuper600 (methylparathion) Comp. Biochem. Physiol. C 143 2006 141 149
M. Nolting, B. Ueberscha, and H. Rosenthal Trypsin activity and physiological aspects in larval rearing of European sea bass (Dicentrarchus labrax) using live prey and compound diets J. Appl. Ichthyol. 15 1999 138 142
Y. Oozeki, and K.M. Bailey Ontogenetic development of digestive enzyme activities in larval walleye pollock, Theragra chalcogramma Mar. Biol. 122 1995 177 186
A.K. Pal, and S.K. Konar Chronic effects of the organophosphorus insecticide DDVP on feeding, survival, growth and reproduction of fish Environ. Ecol. 3 1985 398 402
S. Pena-Llopis, M.D. Ferrando, and J.B. Pena Fish tolerance to organophosphate-induced oxidative stress is dependent on the glutathione metabolism and enhanced by N-acetylcysteine Aquat. Toxicol. 65 2003 337 360
M.W. Pfaffl A new mathematical model for relative quantification in real-time RT-PCR Nucleic Acids Res. 29 2001 2002 2007
S. Pfeifer, D.S. Schiedek, and J.W. Dippner Effect of temperature and salinity on acetylcholinesterase activity, a common pollution biomarker, in Mytilus sp. from the south-western Baltic Sea J. Exp. Mar. Biol. Ecol. 320 2005 93 103
J.W. Pridgeon, and N. Liu Overexpression of the Cytochrome c oxidase subunit I gene associated with a pyrethroid resistant strain of German cockroaches, Blattella germanica (L.). Insect Biochem Mol. Biol. 33 2003 1043 1048
L. Ribeiro, J.L. Zambonino-Infante, C. Cahu, and M.T. Dinis Development of digestive enzymes in larvae of Solea senegalensis, Kaup 1858 Aquaculture 179 1999 465 473
C. Richter Reactive oxygen species and nitrogen species regulate mitochondrial Ca2+ homeostasis and respiration Biosci. Rep. 17 1997 53 66
E.L. Rodrigues, M.J.T. Ranzani-Paiva, F.J. Pacheco, and M.L. Veiga Histopathologic lesions in the liver of Prochilodus lineatus (Pisces, Prochilodontidae) exposed to a sublethal concentration of the organophosphate insecticide Dipterex500s (Trichlorfon) Acta Sci. 23 2001 503 505
K. Ruddle, and G. Zhong Integrated Agriculture-Aquaculture in South China: The Dike-Pond System of the Zhujiang Delta 1988 Cambridge University Press Cambridge
E. Sancho, M.D. Ferrando, and E. Andreu-Moliner Response and recovery of brain acetylcholinesterase activity in the European eel, Anguilla anguilla, exposed to fenitrothion Ecotoxicol. Environ. Saf. 38 1997 205 209 (Pubitemid 28082353)
M.C. Sarasquete, A. Polo, and M.L. Gonzalez de Canales A histochemical and immunohistochemical study of digestive enzymes and hormones during the larval development of the sea bream, Sparus aurata L Histochem. J. 25 1993 430 437
A. Sarkar, D. Ray, A.N. Shrivastava, and S. Sarker Molecular biomarkers: their significance and application in marine pollution monitoring Ecotoxicology 15 2006 333 340
T. Shimada, C.L. Hayes, H. Yamazaki, S. Amin, S.S. Hecht, and F.P. Guengerich Activation of chemically diverse procarcinogens by human cytochrome P-450 1B1 Cancer Res. 56 1996 2979 2984
G. Sievers, P. Palacios, R. Inostroza, and H. D'olz Evaluation of the toxicity of 8 insecticides in Salmo salar and the in vitro effects against the isopode parasite, Ceratothoa gaudichaudii Aquaculture 134 1995 9 16
H.C. Silva, H.S.G. Medina, E. Fanta, and M. Bacila Sub-lethal effects of the organophosphate Folidol 600 (Methyl Parathion) on Callichthys callichthys (Pisces, Teleostei) Comp. Biochem. Physiol. C 105 1993 197 201
R. Smolder, L. Bervoets, V. Wepener, and R. Blust A conceptual framework for using mussels as biomonitors in whole effluent toxicity Hum. Ecol. Risk Assess. 9 2003 741 760
J.J. Stegeman, M. Brouwer, R.T. Di Giulio, L. Forlin, B.A. Fowler, B.M. Sanders, and P.A. Van Veld Molecular responses to environmental contamination: enzyme and protein systems as indicators of chemical exposure and effect R.T. Di Giulio, L. Forlin, B.A. Fowler, B.M. Sanders, P.A. Van Veld, R.J. Huggett, R.A. Kimerle, P.M. Merhle, H.L. Bergman, Biomarkers: Biochemical, Physiological and Histological Markers of Anthropogenic Stress. A Special Publication of SETAC 1992 Lewis Publishers Chelsea, MI 235 335
A. Sturm, H.C. Silva de Assis, and P.D. Hansen Cholinesterases of marine teleost fish: enzymological characterization and potential use in the monitoring of neurotoxic contamination Mar. Environ. Res. 47 1999 389 398
A. Sturm, J. Wogram, H. Segner, and M. Liess Different sensitivity to organophosphates of acetylcholinesterase and butyrylcholinesterase from three-spined stickleback (Gasterosteus aculeatus): application in biomonitoring Environ. Toxicol. Chem. 19 2000 1607 1617
J.M. Thomaz, N.D. Martins, D.A. Monteiro, F.T. Rantin, and A.L. Kalinin Cardio-respiratory function and oxidative stress biomarkers in Nile tilapia exposed to the organophosphate insecticide trichlorfon (NEGUVON®) Ecotoxicol. Environ. Saf. 72 2009 1413 1424
J.L. Tojo, and M.T. Santamarina Oral pharmacological treatments for parasitic diseases of rainbow trout Oncorhynchus mykiss. III Ichthyobodo necator Dis. Aquat. Org. 33 1998 195 199
J. Tronczynski Programme de recherche sur les produits phytosanitaires en zones littorals et estuariennes Institut Francais de Recherche pour I'Exploitation de la Mer, Brest (IFREMER DR0e90e05-MR) 1990
R. van der Oost, J. Beyer, and N.P.E. Vermeulen Fish bioaccumulation and biomarkers in environmental risk assessment: a review Environ. Toxicol. Pharmacol. 13 2003 57 149
I. Varó, R. Serrano, E. Pitarch, F. Amat, F.J. López, and J.C. Navarro Bioaccumulation of chlorpyrifos through an experimental food Chain: study of protein HSP70 as biomarker of sublethal stress in fish Arch. Environ. Contam. Toxicol. 42 2002 229 235
I. Varó, J.C. Navarro, F. Amat, and L. Guilhermino Effect of dichlorvos on cholinesterase activity of the European sea bass (Dicentrarchus labrax) Pesticide Biochem. Physiol. 75 2003 61 72
I. Varó, J.C. Navarro, B. Nunes, and L. Guilhermino Effects of dichlorvos aquaculture treatments on selected biomarkers of gilthead sea bream (Sparus aurata L.) fingerlings Aquaculture 266 2007 87 96
R.L. Venkateswara, D. Shilpanjali, P. Kavitha, and S.S. Madhavendra Toxic effects of profenofos on tissue acetylcholinesterase and gill morphology in a euryhaline fish, Oreochromis mossambicus Arch. Toxicol. 77 2003 227 232
M.M. Vijayan, C. Pereira, R.B. Forsyth, C.J. Kennedy, and G.K. Iwama Handling stress does not affect the expression of hepatic heat shock protein 70 and conjugation enzymes in rainbow trout treated with β-naphthoflavone Life Sci. 61 1997 117 127
G. Villani, and G. Attardi In vivo control of respiration by cytochrome c oxidase in human cells Free Radic. Biol. Med. 29 2000 202 210
K.L. Willett, S. Ganesan, M. Patel, C. Metzger, S. Quiniou, G. Waldbieser, and B. Scheffler In vivo and in vitro CYP1B mRNA expression in channel catfish Mar. Environ. Res. 62 2006 S332 S336
E. Yarsan, M. Tanyuksel, S. Celik, and A. Aydin Effects of aldicarb and malathion on lipid peroxidation Bull. Environ. Contam. Toxicol. 63 1999 575 581
