[en] The aim of our study was to determine the effect of a rectocolonic preimmunization with ovalbumin on the systemic immune response induced by a subsequent subcutaneous injection of this antigen in Balb/c mice. One rectocolonic, but not intragastric, administration of 25 mg of ovalbumin induced a detectable increase in serum anti-ovalbumin antibody level. The level reached was however much lower than after subcutaneous injection. Both intragastric and rectocolonic immunization with ovalbumin induced specific systemic cellular tolerance. However, after rectocolonic, but not intragastric, preimmunization there was no systemic humoral tolerance to this antigen. These differences in systemic immune responses after rectocolonic or intragastric administration of ovalbumin could be due to different stimulation of the systemic immune system or to differences between the colonic and small bowel mucosal immune system, which remain to be elucidated.
Disciplines :
Gastroenterology & hepatology
Author, co-author :
Louis, Edouard ; Université de Liège - ULiège > Département des sciences cliniques > Hépato-gastroentérologie
Franchimont, D.
Lamproye, Anne ; Centre Hospitalier Universitaire de Liège - CHU > Gastro-Entérologie-Hépatologie
Van Kemseke, Catherine ; Centre Hospitalier Universitaire de Liège - CHU > Gastro-Entérologie-Hépatologie
Schaaf-Lafontaine, Nicole ; Centre Hospitalier Universitaire de Liège - CHU > Hématologie biologique et immuno hématologie
Mahieu, P.
Belaiche, Jacques ; Université de Liège - ULiège > Département des sciences cliniques > Hépato-gastroentérologie
Language :
English
Title :
Systemic Immune Response after Rectocolonic Administration of Ovalbumin in Mice
Mowai, AM.; The regulation of immune responses do dietary protein antigens. Immunol Today 1987;8:93-98.
Rombout, J W, Block, L J, Lamers, C H, Egberts, E.; Immunization of carp (C yprinus carpio) with a Vibrio anguiliurtim bacterin: Indications for a common mucosal immune system. Dev Comp Immunol 1986;10:341-351.
Lchncr, T, Brookes, R, Fanagiotidi, C, Tao, L, klavinskis, L S, Walker, J, Walker, P, Ward, R, Hussain, L, Gearing, A J H, Adim, S L, Bcryncicr, A I.; T-and B-cell functions and epitope expression in nonhuman primates immunized with si mian immunodeficiency virus amigen bv the rectal route. Proc Natl Acad Sci USA 1993;90: 8638-8642
Forrest B D, Shearman D J, LaBroov, J T.; Specific immune response in human following rectal dc-livtrv Cf livt typhoid vaccine. Vaccine 1990:8 209-212.
Haneberg, B, Kendall D, Amerongcn, H M, Apter, F M, Kraehcnbuhl, I P, Neulra, M R.; Induction of specific immunoglobulin A in the small intestine, eo I on-rectum, and vagina measured by a now method for collection of secretions from local mucosal surfaces. Infect Immun 1994:62: 15-23.
Mowat AM. Ferguson A: Hypersensitivity in the small intestinal mucosa. V Induction of cell-mediated immunity to a dietary antigen. Clin Exp Immunol 1981;43:574-582.
Lamotll AG. Gordon M. Ferguson A: Oral tolerance in protein-deprived mice. I. Profound antibody tolerance but impaired DTH tolerance alter antigen feeding. Immunology 1987;61:333-338.
Bland PW, Warren LG: Antigen presentation by epithelial cells of the rat small intestine. II. Selective induction of suppressor T cells. Immunology 1986;58:9-14.
Richman LK, Chiller JM, Brown WR, Hanson DG. Vaz NM: Enierically induced immunologic tolerance. I. Induction of suppressor T lymphocytes by intragastric administration of soluble proteins. J Immunol 1978:121:2429-2434.
Mattingly JA: Immunologic suppression after oral administration of antigen. III. Activation of suppressor-inducer cells in the Pcyer's patches. Cell Immunol 1984:86:46-52.
Whitairc, C C, Gienapp, I E, Orosz, C G, Bitar, D M.; Oral tolerance in experimental autoimmune encephalomyelitis. III. Evidence for clonal anergy. J Immunol 1991:147:2155-2163.
Bruce MG. Ferguson A: The influence of intestinal processing on the immunogenicity and molecular size of absorbed, circulating ovalbumin in mice. Immunology 1986:59:295-300.
Beagley K W, Fujihashi K, Lagoo, A S, Elson, C O.; Regional difference in mucosal lymphoid cells of murine small versus large bowel (abstract). Gastroenterology 1992; 102:A 593.
Shamianov, A, McGhce, J R, Beagley K, Fujihashi K, Kiyono, H, Lagoo, A, Llson, C O.; Cytokine secretion by intraepithelial and lamina propria lymphocytes from murine small bowel and large bowel (abstract) Gastroenterology 1993: 104:A780.