[en] Implantation and placental development are dependent upon trophoblast invasion of the endometrium. While the villous trophoblast does not display invasive behavior, the extravillous cytotrophoblast is highly invasive. By cloning BeWo choriocarcinoma cells, we have isolated two distinct clones that share similarities with villous and extravillous cytotrophoblasts. When cultured at the surface of a type I collagen gel, BeWo MC-1 cells were not invasive, whereas BeWo MC-2 cells rapidly invaded this matrix. When injected subcutaneously in nude mice, BeWo MC-1 cells developed a localized tumor and BeWo MC-2 cells developed larger tumors with micrometastases. Gelatinase A expression and minute amounts of gelatinase B were detected in the parental cell line and in both clones. However, the parental and the BeWo MC-2 cells secreted 5- to 10-fold more gelatinase A than the BeWo MC-1 cells. Laminin and matrigel stimulated the production of gelatinase A in BeWo MC-2 cells. Type I collagen promoted the conversion of the 72-kDa progelatinase A in an active enzyme only in parental BeWo and in BeWo MC-2 cells. These clones provide an interesting model for studying the complex mechanisms regulating implantation as well as the controlled invasiveness during implantation compared to tumor invasion.
Disciplines :
Biochemistry, biophysics & molecular biology
Author, co-author :
Crescimanno, C.
Foidart, Jean-Michel ; Université de Liège - ULiège > Département des sciences cliniques > Gynécologie - Obstétrique - Labo de biologie des tumeurs et du développement
Noël, Agnès ; Université de Liège - ULiège > Département des sciences biomédicales et précliniques > Biologie cellulaire et moléculaire appliquée à l'homme
Polette, M.
Maquoi, Erik ; Université de Liège - ULiège > Département des sciences cliniques > Labo de biologie des tumeurs et du développement
Birembaut, P.
Baramova, Eugéenia N.
Kauffmann, P.
Castellucci, M.
Language :
English
Title :
Cloning of choriocarcinoma cells shows that invasion correlates with expression and activation of gelatinase A
1. Kurman, R. J., Main, C. S., and Chen, H. C. (1984) Placenta 5, 349-370.
2. Benirschke, K., and Kaufmann, P. (1990) Pathology of the Human Placenta, Springer-Verlag, New York.
3. Lala, P. K., and Graham, C. H. (1990) Cancer Metast. Rev. 9, 369-379.
4. Reich, R., Thompson, E. W., Iwamoto, Y., Martin, GR. Deason, J. R., Fuller, G. C., and Miskin, R. (1988) Cancer Res. 48, 3307-3312.
5. Librach, C. L., Werb, Z., Fitzgerald, M. L., Chiu, K., Corwin, N. M., Esteves, R. A., Grobelny, D., Galardy, R., Damsky, C. H., and Fisher, S. J. (1991) J. Cell Biol. 113, 437-449.
6. Woessner, J. F., Jr. (1991) FASEB J. 5, 2145-2154.
7. Bischof, P., and Martelli, M. (1992) Placenta 13, 17-24.
8. Strickland, S., and Richards, W. G. (1992) Cell 71, 355-357.
9. Brenner, C. A., Adler, R. R., Rappolee, D. A., Pedersen, R. A., and Werb, Z. (1989) Genes Dev. 3, 848-859.
10. Birkendal-Hansen, H. (1995) Curr. Opin. Cell Biol. 7, 728-735.
11. Docherty, A. J., and Murphy, G. (1990) Ann. Rheum. Dis. 49, 469-479.
12. Ray, J. M., and Stetler-Stevenson, W. G. (1994) Eur. Respir. J. 7, 2062-2072.
13. Schultz, R. M., Silberman, S., Persky, B., Bajkowski, A. S., and Carmichael, D. F. (1988) Cancer Res. 48, 5539-5545.
14. Alexander, C. M., and Werb, Z. (1992) J. Cell Biol. 118, 727-739.
15. Behrendtsen, O., Alexander, C. M., and Werb, Z. (1992) Development 114, 447-456.
16. MacDougall, J. R., and Matrisian, L. M. (1995) Cancer Metastasis Rev. 14, 351-362.
17. Emonard, H., Remacle, A. G., Noël, A. C., Grimaud, J. A., Stetler-Stevenson, W. G., and Foidart, J. M. (1992) Cancer Res. 52, 5845-5848.
18. Autio-Harmainen, H., Hurskainen, T., Niskasaari, K., Hoyhtya, M., and Tryggvason, K. (1992) Lab. Invest. 67, 191-200.
19. Polette, M., Nawrocki, B., Pintiaux, A., Massenat, C., Maquoi, E., Volders, L., Schaaps, J. P., Birembaut, P., and Foidart, J. M. (1994) Lab. Invest. 71, 838-846.
20. Douglas, G. C., and King, B. J. (1989) J. Immunol. Meth. 119, 259-268.
21. Loke, Y. W., Gardner, L., and Grabowska, A. (1989) Placenta 10, 407-415.
22. Mazur, M. T., and Kurman, R. J. (1987) in Blaustein's Pathology of Female Genital Tract, pp. 835-875, Springer-Verlag, New York.
23. Pattillo, R. A., and Gey, G. O. (1968) Cancer Res. 28, 1231-1236.
24. Timpl, R., Rohde, H., Robey, P. G., Rennard, S. I., Foidart, J. M., and Martin, G. R. (1979) J. Biol. Chem. 254, 9933-9937.
25. Kleinman, H. K., Mc Garvey, M. L., Hassell, J. R., Star, V. L., Cannon, F. B., and Laurie, G. W. (1986) Biochemistry 25, 312-318.
26. Emonard, H., Callé, A., Grimaud, J. A., Peyrol, S., Castronovo, V., Noël, A., Kleinman, H. K., and Foidart, J. M. (1987) J. Invest. Dermatol. 89, 156-163.
27. Pennline, K. (1981) in Manual of Macrophage Methology: Collection, Characterization and Function (Herscowitz, H. V., Holden, T. H., Bellanti, J. A., and Gahffar, A., Eds.), pp. 63-68, Dekker, New York.
28. Noël, A., Simon, N., Raus, J., and Foidart, J. M. (1992) Biochem. Pharmacol. 43, 1263-1267.
29. Attia, M. A., and Weiss, D. W. (1966) Cancer Res. 26, 1787-1800.
30. Brown, P. D., Kleiner, D. E., Unsworth, E. J., and Stetler-Stevenson, W. G. (1993) Kidney Int. 43, 163-170.
31. Noël, A., Munaut, C., Nusgens, B., Lapière, Ch. M., and Foidart, J. M. (1993) Invasion Metastasis 13, 72-81.
32. Emonard, H., Takiya, C., Dreze, S., Cordier, J. F., and Grimaud, J. A. (1989) Matrix 9, 383-388.
33. Salo, T., Liotta, L. A., and Tryggvason, K. (1983) J. Biol. Chem. 258, 3058-3063.
34. Lefebvre, V., Peeters-Joris, C., and Vaes, G. (1991) Biochim. Biophys. Acta 1094, 8-18.
35. Chomczynski, P., and Sacchi, N. (1987) Anal. Biochem. 162, 156-159.
36. Noël, A., Munaut, C., Boulvain, A., Calberg-Bacq, C. M., Nusgens, B., Lapière, Ch. M., and Foidart, J. M. (1992) J. Cell. Biochem. 48, 150-161.
37. Aplin, J. D., and Jones, C. J. P. (1989) in Placenta as a Model and a Source (Genbacev, O., Klopper, A., and Beaconsfields, R., Eds.), pp. 115-128, Plenum, New York.
38. Roskelley, C. D., Srebrow, A., and Bissell, M. J. (1995) Curr. Opin. Cell Biol. 7, 736-747.
39. Bischof, P., Haenggeli, L., and Campana, A. (1995) Mol. Hum. Reprod. 10, 734-742.
40. Aplin, J. D., Sattar, A., and Mould, A. P. (1992) J. Cell Sci. 103, 435-444.
41. Zhou, Y., Damsky, C. H., Chiu, K., Roberts, J. M., and Fisher, S. J. (1993) J. Clin. Invest. 91, 950-960.
42. Damsky, C. H., Librach, C., Lim, K. H., Fitzgerald, M. L., McMaster, M. T., Janatpour, M., Zhou, Y., Logan, S. K., and Fisher, S. J. (1994) Development 120, 3657-3666.
43. Loke, Y. W., and King, A. (1995) in Human Reproduction: Cell Biology and Immunology (Loke, Y. W., and King, A., Eds.), pp. 151-179, Cambridge Univ. Press, UK.
44. Grummer, R., Hohn, H. P., Mareel, M. M., and Denker, H. W. (1994) Placenta 15, 411-429.
45. Damsky, C. H., Fitzgerald, M. L., and Fisher, S. J. (1992) J. Clin. Invest. 89, 210-222.
46. Castellucci, M., Crescimanno, C., Schroeter, C. A., Kaufmann, P., and Muhlhauser, J. (1993) in Frontiers in Gynecologic and Obstetrics Investigation (Genazzani, A. R., Petraglia, F., and Genazzani, A. D., Eds.), pp. 19-25, Parthenon, New York.
47. Castellucci, M., Scheper, M., Scheffen, I., Celona, A., and Kaufmann, P. (1990) Anat. Embryol. 181, 117-128.
48. Kosanke, G., Castellucci, M., Kaufmann, P., and Mironov, V. A. (1993) Placenta 14, 591-604.
49. Ward, R. B., Hembry, R. M., Reynolds, J. J., and Murphy, G. (1991) Biochem. J. 278, 179-187.
50. Azzam, H. S., and Thompson, E. W. (1992) Cancer Res. 52, 4540-4544.
51. Azzam, H. S., Arand, G., Lippmann, M. E., and Thompson, E. W. (1993) J. Natl. Cancer Inst. 85, 1758-1764.
52. Emonard, H., Christiane, Y., Smet, M., Grimaud, J. A., and Foidart, J. M. (1990) Invasion Metastasis 10, 170-177.
53. Turpeenniemi-Hujanen, T., Thorgeirsson, V. P., Rao, C. N., and Liotta, L. A. (1986) J. Biol. Chem. 261, 1883-1889.
54. Fridman, R., Kibbey, M. C., Royce, L. S., Zain, M., Sweeny, T. M., Jicha, D. L., Yannelli, J. R., Martin, G. R., and Kleinman, H. K. (1991) J. Natl. Cancer Inst. 83, 769-774.