Reference : Regulation of neural markers nestin and GFAP expression by cultivated bone marrow str...
Scientific journals : Article
Life sciences : Multidisciplinary, general & others
Life sciences : Biochemistry, biophysics & molecular biology
Regulation of neural markers nestin and GFAP expression by cultivated bone marrow stromal cells.
Wislet-Gendebien, Sabine mailto [Université de Liège - ULiège > Département des sciences biomédicales et précliniques > Biochimie et physiologie générales, et biochimie humaine >]
Leprince, Pierre mailto [Université de Liège - ULiège > > CNCM/ Centre fac. de rech. en neurobiologie cell. et moléc. >]
Moonen, Gustave mailto [Centre Hospitalier Universitaire de Liège - CHU > > Neurologie Sart Tilman >]
Rogister, Bernard mailto [Centre Hospitalier Universitaire de Liège - CHU > > Neurologie Sart Tilman >]
Journal of Cell Science
Company of Biologists
Pt 16
Yes (verified by ORBi)
United Kingdom
[en] Animals ; Bone Marrow Cells/cytology/metabolism ; Cell Differentiation/genetics ; Cell Lineage/genetics ; Cells, Cultured ; Coculture Techniques ; Embryo, Mammalian/metabolism ; Glial Fibrillary Acidic Protein/metabolism ; Green Fluorescent Proteins ; Immunohistochemistry ; Intermediate Filament Proteins/metabolism ; Luminescent Proteins ; Mice ; Mice, Inbred C57BL ; Nerve Tissue Proteins ; Neurons/cytology/metabolism ; Phenotype ; Rats
[en] Bone marrow stromal cells can differentiate into many types of mesenchymal cells, i.e. osteocyte, chondrocyte and adipocyte, but can also differentiate into non-mesenchymal cells, i.e. neural cells under appropriate in vivo experimental conditions (Kopen et al., 1999; Brazelton et al., 2000; Mezey et al., 2000). This neural phenotypic plasticity allows us to consider the utilization of mesenchymal stem cells as cellular material in regenerative medicine. In this study, we demonstrate that cultured adult rat stromal cells can express nestin, an intermediate filament protein predominantly expressed by neural stem cells. Two factors contribute to the regulation of nestin expression by rat stromal cells: serum in the culture medium inhibits nestin expression and a threshold number of passages must be reached below which nestin expression does not occur. Only nestin-positive rat stromal cells are able to form spheres when they are placed in the culture conditions used for neural stem cells. Likewise, only nestin-positive stromal cells are able to differentiate into GFAP (glial fibrillary acidic protein)-positive cells when they are co-cultivated with neural stem cells. We thus demonstrated that adult rat stromal cells in culture express nestin in absence of serum after passaging the cells at least ten times, and we suggest that nestin expression by these cells might be a prerequisite for the acquisition of the capacity to progress towards the neural lineage.
Centre de neurobiologie cellulaire et moleculaire
Fonds National de la Recherche Scientifique (FNRS) of Belgium ; Fondation Medical Reine Elisabeth (FMRE) ; Fonds Charcot ; Belgian League against Multiple Sclerosis

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