Electrospray Mass Spectrometry to Study Drug-Nucleic Acids Interactions

[en] We present here a tutorial review on the electrospray mass spectrometry technique and its applications to the study of drug-nucleic acid non-covalent complexes. Particular emphasis has been made on the basic principles of the technique, to allow even the non-specialist to design fit-for-purpose mass spectrometry experiments and interpret the results. Standard applications will be described in detail, including the determination of stoichiometries and equilibrium binding constants of non-covalent complexes, the study of binding kinetics, and the development of ligand screening assays. We also outline the potentials of more advanced and/or more recent MS-based techniques (tandem mass spectrometry, ion mobility spectrometry and gas-phase spectroscopy) for the study of the nucleic acid-ligand complexes.

Research center :

CART - Centre Interfacultaire d'Analyse des Résidus en Traces - ULiège
Giga-Systems Biology and Chemical Biology - ULiège

Disciplines :

Chemistry
Biochemistry, biophysics & molecular biology

Author, co-author :

Rosu, Frédéric ; Université de Liège - ULiège > Département de Chimie (sciences) > Chimie physique, spectrométrie de masse

De Pauw, Edwin ; Université de Liège - ULiège > Département de Chimie (sciences) > Chimie physique, spectrométrie de masse

Gabelica, Valérie ; Université de Liège - ULiège > Département de Chimie (sciences) > Chimie physique, spectrométrie de masse

Language :

English

Title :

Electrospray Mass Spectrometry to Study Drug-Nucleic Acids Interactions

Publication date :

2008

Journal title :

Biochimie

ISSN :

0300-9084

eISSN :

1638-6183

Publisher :

Elsevier, Paris, France

Special issue title :

Special issue: Targeting DNA, part I

Volume :

Issue :

Pages :

1074-1087

Peer reviewed :

Peer Reviewed verified by ORBi

Funders :

F.R.S.-FNRS - Fonds de la Recherche Scientifique [BE]

Commentary :

Available on ORBi :

since 28 July 2008

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244 (23 by ULiège)

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1516 (17 by ULiège)

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Scopus citations^®

133

Scopus citations^®
without self-citations

105

OpenCitations

124

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