[en] Mannheimiosis is the major respiratory disease among some ruminants, whereas it is not pathogenic for
other mammals, an observation that has been attributed to a specific interaction between Mannheimia
haemolytica leukotoxin (Lkt) and bovine or ovine CD18 subunit of lymphocyte function-associated
antigen-1 (LFA-1) and Mac-1. We therefore hypothesized that Lkt utilizes CD18 as its receptor on caprine
leukocytes as well. We have transiently transfected the b2-integrins-deficient K-562 cell line with cDNAs
encoding caprine CD11a and caprine CD18 to determine the susceptibility of the transfectants to Lktinduced
cytolysis. Flow cytometric analysis of the transfectants revealed surface expression of caprine
LFA-1 and lysis by Lkt in a concentration-dependent manner whereas the parent cells were not.
Moreover, K562 cells expressing caprine CD18 and human or bovine CD11a were also sensitive to Lkt
whereas K-562 cells expressing caprine CD11a and human CD18 were not. Taken together, these results
indicate that CD18 on caprine leukocytes serves as a receptor for Lkt.
Disciplines :
Veterinary medicine & animal health
Author, co-author :
Fett, Thomas ; Université de Liège - ULiège > Département de morphologie et pathologie > Pathologie spéciale et autopsies
Zecchinon, Laurent ; Université de Liège - ULiège > Département de morphologie et pathologie > Pathologie spéciale et autopsies
Vanden Bergh, Philippe ; Université de Liège - ULiège > Département de morphologie et pathologie > Pathologie spéciale et autopsies
Desmecht, Daniel ; Université de Liège - ULiège > Département de morphologie et pathologie > Pathologie spéciale et autopsies
Language :
English
Title :
Mannheimia haemolytica leukotoxin-induced cytolysis of caprine (Capra hircus) leukocytes is mediated by the CD18 subunit of beta2-integrins
Publication date :
2008
Journal title :
Microbial Pathogenesis
ISSN :
0882-4010
eISSN :
1096-1208
Publisher :
Academic Press, London, United Kingdom
Volume :
45
Pages :
337-342
Peer reviewed :
Peer Reviewed verified by ORBi
Funders :
FRIA - Fonds pour la Formation à la Recherche dans l'Industrie et dans l'Agriculture
scite shows how a scientific paper has been cited by providing the context of the citation, a classification describing whether it supports, mentions, or contrasts the cited claim, and a label indicating in which section the citation was made.
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