Article (Scientific journals)
Mannheimia haemolytica leukotoxin-induced cytolysis of caprine (Capra hircus) leukocytes is mediated by the CD18 subunit of beta2-integrins
Fett, Thomas; Zecchinon, Laurent; Vanden Bergh, Philippe et al.
2008In Microbial Pathogenesis, 45, p. 337-342
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Keywords :
Caprine; Mannheimia haemolytica
Abstract :
[en] Mannheimiosis is the major respiratory disease among some ruminants, whereas it is not pathogenic for other mammals, an observation that has been attributed to a specific interaction between Mannheimia haemolytica leukotoxin (Lkt) and bovine or ovine CD18 subunit of lymphocyte function-associated antigen-1 (LFA-1) and Mac-1. We therefore hypothesized that Lkt utilizes CD18 as its receptor on caprine leukocytes as well. We have transiently transfected the b2-integrins-deficient K-562 cell line with cDNAs encoding caprine CD11a and caprine CD18 to determine the susceptibility of the transfectants to Lktinduced cytolysis. Flow cytometric analysis of the transfectants revealed surface expression of caprine LFA-1 and lysis by Lkt in a concentration-dependent manner whereas the parent cells were not. Moreover, K562 cells expressing caprine CD18 and human or bovine CD11a were also sensitive to Lkt whereas K-562 cells expressing caprine CD11a and human CD18 were not. Taken together, these results indicate that CD18 on caprine leukocytes serves as a receptor for Lkt.
Disciplines :
Veterinary medicine & animal health
Author, co-author :
Fett, Thomas ;  Université de Liège - ULiège > Département de morphologie et pathologie > Pathologie spéciale et autopsies
Zecchinon, Laurent ;  Université de Liège - ULiège > Département de morphologie et pathologie > Pathologie spéciale et autopsies
Vanden Bergh, Philippe ;  Université de Liège - ULiège > Département de morphologie et pathologie > Pathologie spéciale et autopsies
Desmecht, Daniel ;  Université de Liège - ULiège > Département de morphologie et pathologie > Pathologie spéciale et autopsies
Language :
English
Title :
Mannheimia haemolytica leukotoxin-induced cytolysis of caprine (Capra hircus) leukocytes is mediated by the CD18 subunit of beta2-integrins
Publication date :
2008
Journal title :
Microbial Pathogenesis
ISSN :
0882-4010
eISSN :
1096-1208
Publisher :
Academic Press, London, United Kingdom
Volume :
45
Pages :
337-342
Peer reviewed :
Peer Reviewed verified by ORBi
Funders :
FRIA - Fonds pour la Formation à la Recherche dans l'Industrie et dans l'Agriculture [BE]
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