equine; articular chondrocytes; synoviocytes; free radical; electron spin resonance; anoxia
Abstract :
[en] By oxymetry and electron paramagnetic resonance (EPR), we investigated the effects of repeated anoxia/re-oxygenation (A/R) periods on the respiration and production of free radicals by synoviocytes (rabbit HIG-82 cell line and primary equine synoviocytes) and equine articular chondrocytes. Three periods of 20 min anoxia followed by re-oxygenation were applied to 10(7)cells; O(2) consumption was measured before anoxia and after each re-oxygenation. After the last A/R, cellular free radical formation was investigated by EPR spectroscopy with spin trapping technique (n=3 for each cell line). Both types of synoviocytes showed a high O(2) consumption, which was slowered after anoxia. By EPR with the spin trap POBN, we proved a free radical formation. Results were similar for equine and rabbit synoviocytes. For chondrocytes, we observed a low O(2) consumption, unchanged by anoxia, and no free radical production. These observations suggest an oxidant activity of synoviocytes, potentially important for the onset of osteoarthritis.
Research center :
CORD - Centre de l'Oxygène, Recherche et Développement - ULiège Centre Européen du Cheval de Mont-le-Soie
Disciplines :
Biochemistry, biophysics & molecular biology
Author, co-author :
Schneider, Nicole ; Université de Liège - ULiège > Centre de l'oxygène : Recherche et développement (C.O.R.D.)
Mouithys-Mickalad, Ange ; Université de Liège - ULiège > Centre de l'oxygène : Recherche et développement (C.O.R.D.)
Lejeune, Jean-Philippe ; Université de Liège - ULiège > Département clinique des animaux de compagnie et des équidés > Anesthésiologie gén. et pathologie chirurg. des grds animaux
Dupont, Ginette ; Université de Liège - ULiège > Centre de l'oxygène : Recherche et développement (C.O.R.D.)
Hoebeke, Maryse ; Université de Liège - ULiège > Département de physique > Spectroscopie biomédicale
Serteyn, Didier ; Université de Liège - ULiège > Département clinique des animaux de compagnie et des équidés > Anesthésiologie gén. et pathologie chirurg. des grds animaux
Language :
English
Title :
Synoviocytes, not chondrocytes, release free radicals after cycles of anoxia/re-oxygenation
Publication date :
26 August 2005
Journal title :
Biochemical and Biophysical Research Communications
ISSN :
0006-291X
eISSN :
1090-2104
Publisher :
Academic Press Inc Elsevier Science, San Diego, United States - California
Volume :
334
Issue :
2
Pages :
669-673
Peer reviewed :
Peer reviewed
Funders :
Région wallonne. Ministère de l'Agriculture, de la Nature, de la Forêt, de la Ruralité, du Tourisme, du Patrimoine [BE]
C.W. McIlwraith General pathophysiology of the joint and response to injury C.W. McIlwraith G.W. Wayne Joint Disease in the Horse 1996 W.B. Saunders Philadelphia 43 Chapter 3
R.E. Allen, D.R. Blake, N.B. Nazhat, and P. Jones Superoxide radical generation by inflamed human synovium after hypoxia Lancet 2 1989 282 283
M.J. Grimshaw, and R.M. Mason Bovine articular chondrocyte function in vitro depends upon oxygen tension Osteoarthritis Cartilage 8 2000 386 392
N. Schneider, J.P. Lejeune, C. Deby, G.P. Deby-Dupont, and D. Serteyn Viability of equine articular chondrocytes in alginate beads exposed to different oxygen tensions Vet. J. 168 2004 167 173
A. Mouithys-Mickalad, M. Mathy-Hartert, G. Du, F. Sluse, C. Deby, and M. Lamy Oxygen consumption and electron spin resonance studies of free radical production by alveolar cells exposed to anoxia: inhibiting effects of the antibiotic ceftazidime Redox. Report 7 2002 85 94
G. Du, K. Willet, A. Mouithys-Mickalad, C. Sluse-Goffart, M.T. Droy-Lefaix, and F. Sluse EGb 761 protects liver mitochondria against injury induced by in vitro anoxia/re-oxygenation Free Radic. Biol. Med. 27 1999 596 604
G. Du, A. Mouithys-Mickalad, and F. Sluse Generation of superoxide anion by mitochondria and impairment of their functions during anoxia and re-oxygenation in vitro Free Radic. Biol. Med. 25 1998 1066 1074
G. Loschen, L. Flohe, and B. Chance Respiratory chain linked H 2O2 production in pigeon heart mitochondria FEBS Lett. 18 1971 261 264
A.Y. Andreyev, Y.E. Kushnareva, and A.A. Starkov Mitochondrial metabolism of reactive oxygen species Biochemistry (Moscow) 70 2005 200 214
J. Vasquez-Vivar, B. Kalyanarama, and M.C. Kennedy Mitochondrial aconitase is a source of hydroxyl radical. An electron spin resonance investigation J. Biol. Chem. 275 2000 14064 14069
P. Ghafourifar, and E. Cadenas Mitochondrial nitric oxide synthase Trends Pharmacol. Sci. 26 2005 190 195
M.L. Tiku, Y.P. Yan, and K.Y. Chen Hydroxyl radical formation in chondrocytes and cartilage as detected by electron paramagnetic resonance spectroscopy using spin trapping reagents Free Radic. Res. 29 1998 177 187
Y. Kawai, E. Kubota, and E. Okabe Reactive oxygen species participation in experimentally induced arthritis of the temporomandibular joint in rats J. Dent. Res. 79 2000 1489 1495
