equine; articular chondrocytes; synoviocytes; free radical; electron spin resonance; anoxia
Abstract :
[en] By oxymetry and electron paramagnetic resonance (EPR), we investigated the effects of repeated anoxia/re-oxygenation (A/R) periods on the respiration and production of free radicals by synoviocytes (rabbit HIG-82 cell line and primary equine synoviocytes) and equine articular chondrocytes. Three periods of 20 min anoxia followed by re-oxygenation were applied to 10(7)cells; O(2) consumption was measured before anoxia and after each re-oxygenation. After the last A/R, cellular free radical formation was investigated by EPR spectroscopy with spin trapping technique (n=3 for each cell line). Both types of synoviocytes showed a high O(2) consumption, which was slowered after anoxia. By EPR with the spin trap POBN, we proved a free radical formation. Results were similar for equine and rabbit synoviocytes. For chondrocytes, we observed a low O(2) consumption, unchanged by anoxia, and no free radical production. These observations suggest an oxidant activity of synoviocytes, potentially important for the onset of osteoarthritis.
Research Center/Unit :
CORD - Centre de l'Oxygène, Recherche et Développement - ULiège Centre Européen du Cheval de Mont-le-Soie
Disciplines :
Biochemistry, biophysics & molecular biology
Author, co-author :
Schneider, Nicole ; Université de Liège - ULiège > Centre de l'oxygène : Recherche et développement (C.O.R.D.)
Mouithys-Mickalad, Ange ; Université de Liège - ULiège > Centre de l'oxygène : Recherche et développement (C.O.R.D.)
Lejeune, Jean-Philippe ; Université de Liège - ULiège > Département clinique des animaux de compagnie et des équidés > Anesthésiologie gén. et pathologie chirurg. des grds animaux
Dupont, Ginette ; Université de Liège - ULiège > Centre de l'oxygène : Recherche et développement (C.O.R.D.)
Hoebeke, Maryse ; Université de Liège - ULiège > Département de physique > Spectroscopie biomédicale
Serteyn, Didier ; Université de Liège - ULiège > Département clinique des animaux de compagnie et des équidés > Anesthésiologie gén. et pathologie chirurg. des grds animaux
Language :
English
Title :
Synoviocytes, not chondrocytes, release free radicals after cycles of anoxia/re-oxygenation
Publication date :
26 August 2005
Journal title :
Biochemical and Biophysical Research Communications
ISSN :
0006-291X
eISSN :
1090-2104
Publisher :
Academic Press Inc Elsevier Science, San Diego, United States - California
Volume :
334
Issue :
2
Pages :
669-673
Peer reviewed :
Peer reviewed
Funders :
Région wallonne. Ministère de l'Agriculture, de la Nature, de la Forêt, de la Ruralité, du Tourisme, du Patrimoine
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