Article (Scientific journals)
Impact of a mutation in the mitochondrial LSU rRNA gene from Chlamydomonas reinhardtii on the activity and the assembly of respiratory-chain complexes
Remacle, Claire; Gloire, Geoffrey; Cardol, Pierre et al.
2004In Current Genetics, 45 (5), p. 323-330
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Keywords :
mutation; ribosomal RNA; GTPase domain; Chlamydomonas reinhardtii; mitochondria; respiratory complexes
Abstract :
[en] Two substitutions A1090G and A1098C (together called the m mutation) located in the conserved GTPase domain of the mitochondrial LSU rRNA gene were recently shown to weakly compensate for the phenotypical effect of a -1T frameshift mutation in the mitochondrial cox1 gene of C. reinhardtii. In order to analyze the impact of the m mutation on the mitochondrial translational machinery, a strain carrying the m mutation but wild-type for the cox1 gene was isolated. We found that the growth and the respiratory rate of the m mutant were affected and that the activities of complexes I, III, and IV, all containing mitochondria-encoded subunits, were lowered. In contrast the activities of complex II and of the alternative oxidase, both encoded exclusively by the nuclear genome, were not modified. The steady-state levels of complex I enzyme and of several components of the respiratory complexes I, III, and IV were also reduced in the mutant. We moreover showed that m did not suppress other frameshift or UGA stop mutations which affect mitochondrial genes.
Disciplines :
Genetics & genetic processes
Author, co-author :
Remacle, Claire  ;  Université de Liège - ULiège > Département des sciences de la vie > Génétique
Gloire, Geoffrey ;  Université de Liège - ULiège > Virologie - Immunologie
Cardol, Pierre  ;  Université de Liège - ULiège > Département des sciences de la vie > Biochimie végétale
Matagne, René-Fernand ;  Université de Liège - ULiège > Services généraux (Faculté des sciences) > Relations académiques et scientifiques (Sciences)
Language :
English
Title :
Impact of a mutation in the mitochondrial LSU rRNA gene from Chlamydomonas reinhardtii on the activity and the assembly of respiratory-chain complexes
Publication date :
May 2004
Journal title :
Current Genetics
ISSN :
0172-8083
eISSN :
1432-0983
Publisher :
Springer-Verlag, New York, United States - New York
Volume :
45
Issue :
5
Pages :
323-330
Peer reviewed :
Peer Reviewed verified by ORBi
Available on ORBi :
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