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Étude microscopique de la maturation de l’ovocyte équin dans le follicule quaternaire
Ponthier, Jérôme
2007
 

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Keywords :
Horse; Oocyte; Microscopy
Abstract :
[en] In the mare, oocytes in vitro maturation protocols are still in development. To our knowledge, immature oocyte aspect under light microscopy has not been studied and compared with mature oocyte aspect. The aim of the study is to give a general description of immature oocytes and the relationships between the different aspects. Follicles from ovaries obtained at slaughterhouse are punctured after measurement of their diameter. Different characters, such as oocyte diameter, cumulus aspect, granulosity and polarity of ooplasm are observed under light microscopy. No correlation between oestral activity, follicle maturity, cumulus cells aspect, polarity and granulosity of ooplasm was observed. Thickness of zona pellucida differs between oocytes with polar or non polar ooplasm, but no other parameter studied showed an influence on oocyte diameter. No correlation was observed between follicle diameter and oocyte diameter. Results show that no character observed under light microscopy is related to oocyte follicular origin or to any other character. Further studies have to be done to compare these oocytes with mature oocytes and to find the ultra-structural origin of characters observed under light microscopy.
Research center :
Reproduction des carnivores domestiques et des équidés
Disciplines :
Veterinary medicine & animal health
Author, co-author :
Ponthier, Jérôme ;  Université de Liège - ULiège > Département clinique des animaux de compagnie et des équidés > Département clinique des animaux de compagnie et des équidés
Language :
French
Title :
Étude microscopique de la maturation de l’ovocyte équin dans le follicule quaternaire
Alternative titles :
[fr] Microscopic study of equine oocyte maturation inside quaternary follicle
Defense date :
12 September 2007
Institution :
ULiège - Université de Liège
Degree :
Diplôme d'étude approfondies en Sciences Vétérinaire option Médecine Vétérinaire
Promotor :
Beckers, Jean-François  ;  Université de Liège - ULiège > Département des sciences fonctionnelles (DSF)
President :
Balligand, Marc ;  Université de Liège - ULiège > Département d'Enseignement et de Clinique des animaux de Compagnie (DCC)
Jury member :
Ectors, Fabien  ;  Université de Liège - ULiège > Fundamental and Applied Research for Animals and Health (FARAH) > FARAH: Santé publique vétérinaire
Touati, Kamal ;  Université de Liège - ULiège > Fundamental and Applied Research for Animals and Health (FARAH) > FARAH: Productions animales durables
Hanzen, Christian  ;  Université de Liège - ULiège > Département d'Enseignement et de Clinique des animaux de Production (DCP)
Antoine, Nadine ;  Université de Liège - ULiège > Fundamental and Applied Research for Animals and Health (FARAH) > FARAH: Santé publique vétérinaire
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since 05 January 2009

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