[en] The cloning and sequencing of the rbpa gene coding for a versatile peroxidase from a novel Bjerkandera strain is hereby reported. The 1777 bp isolated fragment contained a 1698 bp peroxidase-encoding gene, interrupted by 11 introns. The 367 amino acid-deduced sequence includes a 27 amino acid-signal peptide. The molecular model, built via homology modelling with crystal structures of four fungal peroxidases, highlighted the amino acid residues putatively involved in manganese binding and aromatic substrate oxidation. The potential heme pocket residues (R44, F47, H48, E79, N85, H177, F194 and D239) include both distal and proximal histidines (H48 and H177). RBP possesses potential calcium-binding residues (D49, G67, D69, S71, S178, D195, T197, I200 and D202) and eight cysteine residues (C3, C15, C16, C35, C121, C250, C286, C316). In addition, RIBP includes residues involved in substrate oxidation: three acidic residues (E37, E41 and D183)-putatively involved in manganese binding and H83 and W172-potentially involved in oxidation of aromatic substrates. Characterisation of nucleotide and amino acid sequences include RBP in versatile peroxidase group sharing catalytic properties of both UP and MnP. In addition, the RBP enzyme appears to be closely related with the ligninolytic peroxidases from the Trametes versicolor strain. (C) 2005 Published by Elsevier B.V.
Disciplines :
Microbiology Biotechnology
Author, co-author :
Moreira, Patricia R.
Duez, Colette ; Université de Liège - ULiège > Centre d'ingénierie des protéines
Dehareng, Dominique ; Université de Liège - ULiège > Centre d'ingénierie des protéines
Antunes, Antonio; Université de Liège - ULiège > Chimie générale et Physique > Spectrométrie de masse
Almeida-Vara, E.; INETI, Lisboa Portugal > Biotecnologia
Frère, Jean-Marie ; Université de Liège - ULiège > Département des sciences de la vie > Département des sciences de la vie
Malcata, F.Xavier; Universidade Catolica Portuguesa, Porto > Escola superior de biotecnologia
Duarte, J. C.; INETI, Lisboa Portugal > Biotecnologia
Language :
English
Title :
Molecular characterisation of a versatile peroxidase from a Bjerkandera strain
Publication date :
10 September 2005
Journal title :
Journal of Biotechnology
ISSN :
0168-1656
eISSN :
1873-4863
Publisher :
Elsevier Science Bv, Amsterdam, Netherlands
Volume :
118
Issue :
4
Pages :
339-352
Peer reviewed :
Peer Reviewed verified by ORBi
Funders :
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