Article (Scientific journals)
Effect of Intravenous Platelet-Activating Factor on Bovine Pulmonary Mast Cells
Jolly, Sandra; Thomas, Cédric; Genicot, Bruno et al.
2001In Journal of Comparative Pathology, 125 (Aug-Oct), p. 81-89
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Keywords :
platelet; bronchoconstriction; hypertension
Abstract :
[en] The effect of platelet-activating factor (PAF) on bovine pulmonary mast cells (MCs) was investigated in calves aged 1-4 months. PAF was administered over a 5-min period at a rate of 50 ng/kg/min to six animals, which were killed approximately 10 min later. No macroscopical lesions were observed in the lung tissue of five control calves. On semi-thin toluidine-blue stained sections, MC cytoplasm showed profuse deep-blue granulation. In the lung tissues of PAF-treated calves, striking interstitial, alveolar and interlobular oedema and emphysema were observed. MC granules occurred at the periphery of the cytoplasm and MC nuclei were easily detectable. Moreover, the number of granules in the MC cytoplasm was remarkably low. A few granules with some metachromatic material were sometimes detected outside the cell limits. Ultrastructurally, the matrix pattern of the MC granules in lung tissues of control calves was either amorphous or granular. In lung tissues of the PAF-treated calves, the granular matrix pattern was pronounced, varying from fine- to coarse-grained, and the MCs were of reduced size, with many filiform processes and exocytotic vesicles. These findings indicate that pulmonary MC degranulation was induced by PAF in healthy calves.
Disciplines :
Veterinary medicine & animal health
Author, co-author :
Jolly, Sandra ;  Université de Liège - ULiège > Département de Morphologie et Pathologie > Pathologie spéciale et autopsies
Thomas, Cédric
Genicot, Bruno
Dessy-Doize, Cécile
Coignoul, Freddy ;  Université de Liège - ULiège > Services administratifs généraux > Vice-Recteur à la Gestion de la qualité - Pathologie générale et autopsies
Desmecht, Daniel ;  Université de Liège - ULiège > Département de Morphologie et Pathologie > Pathologie spéciale et autopsies
Language :
English
Title :
Effect of Intravenous Platelet-Activating Factor on Bovine Pulmonary Mast Cells
Publication date :
2001
Journal title :
Journal of Comparative Pathology
ISSN :
0021-9975
Publisher :
W. B. Saunders Co., United Kingdom
Volume :
125
Issue :
Aug-Oct
Pages :
81-89
Peer reviewed :
Peer Reviewed verified by ORBi
Available on ORBi :
since 08 January 2010

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