Longitudinal single-cell RNA sequencing reveals a heterogeneous response of plasma cells to colonic inflammation.

Chchd10; Colitis; Plasma cells; Single-cell RNA sequencing; Ube2c; Dextran Sulfate; Reactive Oxygen Species; Animals; Mice; Male; Mice, Inbred C57BL; Disease Models, Animal; Gene Expression Regulation; Reactive Oxygen Species/metabolism; Single-Cell Analysis/methods; Colitis/genetics; Colitis/chemically induced; Colitis/pathology; Colitis/immunology; Plasma Cells/metabolism; Plasma Cells/pathology; Plasma Cells/immunology; Inflammation/genetics; Inflammation/pathology; Sequence Analysis, RNA; Colon/pathology; C57BL/6 mice; Distilled water; Dynamic changes; Single cells; Sulphates; Colon; Inflammation; Single-Cell Analysis; Transcription Factors; Food Science; Structural Biology; Biochemistry; Biomaterials; Molecular Biology

Abstract :

[en] A comprehensive understanding of the dynamic changes in plasma cells (PCs) during inflammation remains elusive. In this study, we analyzed the distinct responses of PCs across different phases of inflammation in a dextran sodium sulfate (DSS)-induced mouse colitis model. Six-week-old male C57BL/6 mice were treated with 2.2 % DSS in distilled water for 5 days to induce colitis, and colonic tissues were collected at the peak of inflammation, during recovery, and at the end of the recovery phase. Single-cell RNA sequencing was performed to investigate temporal changes in the gut immune environment. PCs were categorized into six subsets, with Ube2c + PCs displaying notable alterations during various inflammatory phases. Genes such as Pycard, Gpx1, Lgals3, and Chchd10 were significantly expressed in Ube2c + PCs and appeared critical in resolving DSS-induced inflammation. Transcription factors (TFs), including Atf4, Cebpg, Jund, and Klf6, exhibited high regulatory activity in Ube2c + PCs across inflammatory stages. Additionally, we identified an interaction between Chchd10 and C1qbp in PCs, which stabilized C1qbp, reduced reactive oxygen species (ROS) production, and potentially enhanced PC survival and function under inflammatory conditions. This study highlights dynamic quasi-temporal gene expression and TF regulation in PCs during colitis, providing insights for future PC-targeted immunotherapy research.

Disciplines :

Microbiology

Author, co-author :

Wen, Xin; Department of Hematology, Qilu Hospital, Cheeloo College of Medicine, Shandong University, Jinan, Shandong, 250012, China

Li, Wei; Department of Hematology, Qilu Hospital, Cheeloo College of Medicine, Shandong University, Jinan, Shandong, 250012, China

Li, Shifang ; Université de Liège - ULiège

Chen, Dawei; Department of Physiology, School of Basic Medical Sciences, Department of Colorectal Surgery, Qilu Hospital, Cheeloo College of Medicine, Shandong University, Jinan, Shandong, 250012, China

Zheng, Junjie; Department of Physiology, School of Basic Medical Sciences, Department of Colorectal Surgery, Qilu Hospital, Cheeloo College of Medicine, Shandong University, Jinan, Shandong, 250012, China

Wang, Xia; Department of Physiology, School of Basic Medical Sciences, Department of Colorectal Surgery, Qilu Hospital, Cheeloo College of Medicine, Shandong University, Jinan, Shandong, 250012, China

Zhang, Cuiyu; Department of Physiology, School of Basic Medical Sciences, Department of Colorectal Surgery, Qilu Hospital, Cheeloo College of Medicine, Shandong University, Jinan, Shandong, 250012, China

Liu, Yanting; Department of Physiology, School of Basic Medical Sciences, Department of Colorectal Surgery, Qilu Hospital, Cheeloo College of Medicine, Shandong University, Jinan, Shandong, 250012, China

Ning, Yao; Department of Physiology, School of Basic Medical Sciences, Department of Colorectal Surgery, Qilu Hospital, Cheeloo College of Medicine, Shandong University, Jinan, Shandong, 250012, China

Jia, Ruinan; Department of Hematology, Qilu Hospital, Cheeloo College of Medicine, Shandong University, Jinan, Shandong, 250012, China

More authors (5 more)

Language :

English

Title :

Longitudinal single-cell RNA sequencing reveals a heterogeneous response of plasma cells to colonic inflammation.

Publication date :

March 2025

Journal title :

International Journal of Biological Macromolecules

ISSN :

0141-8130

eISSN :

1879-0003

Publisher :

Elsevier B.V., Netherlands

Volume :

294

Pages :

139307

Peer reviewed :

Peer Reviewed verified by ORBi

Additional URL :

https://api.elsevier.com/content/article/PII:S0141813024101183?httpAccept=text/xml

Available on ORBi :

since 22 September 2026

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