Abstract :
[en] Global food security depends critically on sustainable agricultural productivity; however, conventional intensive farming practices cause severe soil degradation and nutrient imbalances, undermining long-term ecological resilience. To address this challenge, we developed a multidimensional evaluation framework for conservation-oriented agricultural practices in ecologically fragile regions, systematically investigating the mechanisms through which they restore soil quality and enhance crop yields.
A comprehensive set of conservation-oriented agricultural practices, encompassing diversified crop rotations, surface organic mulching, and long term no tillage was implemented to evaluate its regulatory effects on soil microenvironments. Field characterization and continuous monitoring indicated that these integrated practices significantly improved the physicochemical properties of the soil. Specifically, the synergistic combination of diversified root exudates and organic surface barriers effectively buffered soil alkalization, restored soil organic carbon pools, and optimized soil hydrothermal conditions, thereby synchronizing nutrient slow release with critical crop demands.
Building upon this improved soil microenvironment, mechanistic studies combining metagenomic sequencing and 15N stable isotope labeling revealed key pathways of microbial regulation and material cycling. The conservation-oriented practices, particularly when augmented with AMF, promoted the targeted assembly of functionally robust microbial networks. This biological intervention effectively alleviated inherent nutrient limitations by upregulating core nitrogen-cycling genes (nirA, hao, narB) and significantly enhancing the bidirectional transfer and crop utilization of straw-derived nitrogen.
Ultimately, these microecological and biogeochemical optimizations translated into superior agronomic and environmental performance. Across diverse cropping systems, the integrated conservation practices not only mitigated potential environmental risks such as greenhouse gas emissions and microplastic pollution but also enhanced climate resilience and substantially increased long term crop yields, with yields up to 41% higher in long term Tibetan barley rotations compared with continuous cropping.
In summary, through microenvironmental reconstruction, targeted microbial regulation, material cycling elucidation, and broad-scale application validation, this study demonstrates the significant advantages of integrated conservation-oriented agricultural practices. It provides a sustainable, efficient, and scientifically grounded paradigm for agricultural green intensification and global food security.
