DNA Transposable Elements; DNA Methylation; Genome, Plant; Gene Expression Regulation, Plant; Triticum/genetics; DNA Transposable Elements/genetics; Triticum; Plant Science
Abstract :
[en] Transposable elements play a pivotal role in genome evolution and phenotypic variation in numerous eukaryotic species1. Helitrons, a recently identified category of transposons, remain poorly understood in terms of epigenetic regulation and real-time mobilization in plants2,3. Here our study reveals that reduced DNA methylation combined with heat stress promotes the mobilization of the Xuan-Feng Helitron family in wheat. Activation is marked by transcription, extrachromosomal circular DNA formation and novel somatic insertions. Genetic segregation and heterologous reconstitution establish Feng8 as the autonomous driver of the Xuan-Feng family mobilization. These findings represent a step forward in the study of active Helitrons and their potential biological functions as well as their role in genome dynamics and their potential use in crop breeding.
Disciplines :
Genetics & genetic processes
Author, co-author :
Peng, Haoran ✱; Crop Genome Dynamics Group, Agroscope, Nyon, Switzerland ; Department of Plant Reproductive Biology and Epigenetics, Max Planck Institute of Molecular Plant Physiology, Potsdam, Germany
Tang, Lijing ✱; Université de Liège - ULiège > Département de gestion vétérinaire des Ressources Animales (DRA) > Génomique animale
Kellenberger, Roman ; Crop Genome Dynamics Group, Agroscope, Nyon, Switzerland
Fossati, Dario ; Field Crop Breeding and Genetic Resources, Agroscope, Nyon, Switzerland
Rimbert, Hélène ; INRAE, GDEC, Université Clermont Auvergne, Clermont-Ferrand, France
Sourdille, Pierre ; INRAE, GDEC, Université Clermont Auvergne, Clermont-Ferrand, France
Ma, Shengwei ; Yazhouwan National Laboratory, Sanya, China
Hickman, Alison B; Laboratory of Molecular Biology, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, MD, USA
Dyda, Fred; Laboratory of Molecular Biology, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, MD, USA
Köhler, Claudia ; Department of Plant Reproductive Biology and Epigenetics, Max Planck Institute of Molecular Plant Physiology, Potsdam, Germany
Charlier, Carole ; Université de Liège - ULiège > Département de gestion vétérinaire des Ressources Animales (DRA)
Choulet, Frédéric ; INRAE, GDEC, Université Clermont Auvergne, Clermont-Ferrand, France
This work was supported by the European Research Council under the European Union\u2019s Horizon 2020 research and innovation programme (725701, BUNGEE, to E.B.); the Agence Nationale de la Recherche and Fonds National Suisse (ANR-21-PRCI-CE02, FNS 310030E_205554 \u2018CropCircle\u2019 to E.B.); the China Scholarship Council Grant (201806990012 to H.P.); the Intramural Program of the National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, USA (to F.D. and A.B.H.); the Fund for Scientific Research\u2014FNRS (PDR grant to C.C., TE-rate project), Belgium; and the Max Planck Society, Germany.
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