Evaluation of the IDEXX Procyte Dx Analyzer for the Measurement of Total and Differential Cellular Counts of Bronchoalveolar Lavage Fluid in Healthy Beagles and Dogs with Lower Respiratory Tract Diseases

Machiels, Hélène; Fastrès, Aline; Biénès, Tom; Lyssens, Aurélie; Schlachet, Alexandra; Tutunaru, Alexandru-Cosmin; Phan, Kim-Thu; Billen, Frédéric; Clercx, Cécile; Roels, Elodie

doi:10.1111/vcp.70132

Article (Scientific journals)

Evaluation of the IDEXX Procyte Dx Analyzer for the Measurement of Total and Differential Cellular Counts of Bronchoalveolar Lavage Fluid in Healthy Beagles and Dogs with Lower Respiratory Tract Diseases

Machiels, Hélène; Fastrès, Aline; Biénès, Tom et al.

2026 • In Veterinary Clinical Pathology

Peer Reviewed verified by ORBi

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https://hdl.handle.net/2268/347409

DOI
10.1111/vcp.70132

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Abstract :

[en] ABSTRACT Background Bronchoalveolar lavage fluid (BALF) analysis is a key diagnostic tool in veterinary respiratory medicine. The IDEXX ProCyte Dx analyzer was developed for blood formulation and cellular counting in various fluids, but its applicability for BALF analysis remains unexplored. Objectives To assess the agreement between manual counting and automated counting using the IDEXX ProCyte Dx analyzer for total cell count (TCC) and differential cell count (DCC) in BALF samples from healthy and diseased dogs, under two pretreatment conditions: mucolytic treatment with dithiothreitol (DTT) or filtration. Methods BALF from 10 healthy beagles and 14 diseased dogs were analyzed to compare TCC and DCC between manual and automated methods across pretreatment conditions. Results In healthy dogs, Bland–Altman analysis on log‐transformed data demonstrated a mean ratio close to 1 and acceptable 95% limits of agreement between manual and automated methods and between pretreatment conditions. In diseased dogs, good agreement was observed between manual and automated methods for filtered samples only; the other comparisons showed poor agreement. In both healthy and diseased dogs, Passing‐Bablok regression analysis did not detect systematic or proportional bias between methods. Automated DCC was inconsistent compared with manual methods in both healthy and diseased dogs. Conclusions The IDEXX ProCyte Dx analyzer can be used for TCC measurement of pre‐treated BALF samples in healthy dogs. In diseased dogs, filtration is preferred over DTT‐treatment for automated counting, and results should be interpreted with caution due to the unpredictable influence of pretreatment on cellular counts. For DCC, automated methods are unreliable.

Disciplines :

Veterinary medicine & animal health

Author, co-author :

Machiels, Hélène ; Université de Liège - ULiège > Fundamental and Applied Research for Animals and Health (FARAH) > FARAH: Médecine vétérinaire comparée

Fastrès, Aline; Department of Small Animals Clinical Sciences, Faculty of Veterinary Medicine, Fundamental and Applied Research for Animals and Health Research Unit (FARAH) University of Liege Liège Belgium

Biénès, Tom ; Department of Small Animals Clinical Sciences, Faculty of Veterinary Medicine, Fundamental and Applied Research for Animals and Health Research Unit (FARAH) University of Liege Liège Belgium

Lyssens, Aurélie ; Université de Liège - ULiège > Fundamental and Applied Research for Animals and Health (FARAH) > FARAH: Médecine vétérinaire comparée

Schlachet, Alexandra ; Université de Liège - ULiège > Département d'Enseignement et de Clinique des animaux de Compagnie (DCC)

Tutunaru, Alexandru-Cosmin ; Université de Liège - ULiège > Fundamental and Applied Research for Animals and Health (FARAH) > FARAH: Médecine vétérinaire comparée

Phan, Kim-Thu ; Université de Liège - ULiège > Département d'Enseignement et de Clinique des animaux de Compagnie (DCC)

Billen, Frédéric ; Université de Liège - ULiège > Fundamental and Applied Research for Animals and Health (FARAH) > FARAH: Médecine vétérinaire comparée

Clercx, Cécile ; Université de Liège - ULiège > Département d'Enseignement et de Clinique des animaux de Compagnie (DCC)

Roels, Elodie ; Université de Liège - ULiège > Fundamental and Applied Research for Animals and Health (FARAH) > FARAH: Médecine vétérinaire comparée

Language :

English

Title :

Publication date :

22 July 2026

Journal title :

Veterinary Clinical Pathology

ISSN :

0275-6382

Publisher :

Wiley

Peer reviewed :

Peer Reviewed verified by ORBi

Additional URL :

https://onlinelibrary.wiley.com/doi/pdf/10.1111/vcp.70132

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