[en] The jasmonates (JA) play key roles in defending mechanical wounding, herbivore attack and various stresses in plants. Lipoxygenase (LOX) is one of the key enzymes in the biosynthesis of JA which also catalyzes the green leafy volatiles synthesis at the first step. Wounding and herbivore attack can induce the expression of LOXs. To investigate the molecular mechanism regulating the LOX genes in tobacco, we isolated and identified the LOX3 gene promoter from Nicotiana tabacum. Analysis from transgenic plants and GUS activity fluorimetric assay revealed that 1847bp of NtLOX3 promoter sequence was sufficient for wounding and methyl jasmonic acid (MeJA) induction. For wounding and MeJA induction, the deletion analysis identified the minimal effective regions which were located in-546bp to-199bp. And this region contained the cis-acting elements of CATG-box (CATG), W-box (C/TTGACY) and a T/G-box (AACGTG), which were thought to play a key role in wounding and MeJA elicitation. These results may provide information for further transcriptional regulation studies of LOX genes.
Disciplines :
Physical, chemical, mathematical & earth Sciences: Multidisciplinary, general & others
Author, co-author :
Xie, Minmin; Key Laboratory for Tobacco Gene Resources, Tobacco Research Institute, Chinese Academy of Agricultural Sciences, Qingdao, China ; Graduate School of Chinese Academy of Agricultural Science, Beijing, China ; Laboratory of Biomass and Green Technologies, Gembloux Agro-Bio Tech, University of Liege, Gembloux, Belgium
Li, Shanshan; Key Laboratory of Natural Products Synthetic Biology of Ethnic Medicinal, State Ethnic Affairs Commission, Yunnan Minzu University, Kunming, China
Li, Zhiyuan; Key Laboratory for Tobacco Gene Resources, Tobacco Research Institute, Chinese Academy of Agricultural Sciences, Qingdao, China
Chen, Mingli; Key Laboratory for Tobacco Gene Resources, Tobacco Research Institute, Chinese Academy of Agricultural Sciences, Qingdao, China
Gong, Daping; Key Laboratory for Tobacco Gene Resources, Tobacco Research Institute, Chinese Academy of Agricultural Sciences, Qingdao, China
Richel, Aurore ; Université de Liège - ULiège > Département GxABT > Chemistry for Sustainable Food and Environmental Systems (CSFES)
Language :
English
Title :
ISOLATION AND CHARACTERIZATION OF THE LIPOXYGENASE-3 PROMOTER IN NICOTIANA TABACUM
Publication date :
2024
Journal title :
Pakistan Journal of Botany
ISSN :
0556-3321
eISSN :
2070-3368
Publisher :
Pakistan Botanical Society
Volume :
56
Issue :
1
Pages :
323 - 330
Peer reviewed :
Peer Reviewed verified by ORBi
Funding text :
This work was supported by the Key Science and Technology Project of the China National Tobacco Corporation [110202201007 (JY-07)].
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