[en] Immunohistochemistry is an indispensable tool in the assessment and characterization of lineage-specific differentiation of grafted cells in cell-based-therapy. This strategy is under investigation for the treatment of many muscle disorders and different animals such as dogs are used as models to study the tissue regeneration. The aim of the present study was to characterize an antibody panel for the analysis of canine muscle cells, useful in routinely processed formalin-fixed paraffin-embedded tissues. Overall, 12 antibodies (8 mouse monoclonal and 4 goat polyclonal), validated for use on human tissues tested for cross-reactivity on canine smooth muscle (bladder, intestine, and uterus), skeletal muscle and heart. Specific staining was achieved with eight antibodies, of which six were cytoplasmic markers (desmin, HDAC8, MHC, SMA, Troponin I and Troponin T) and two were cardiac nuclear markers (GATA-4 and Nkx-2.5). This antibody panel may be useful not only for the evaluation of cell-based therapies in muscle disorders, but also for the evaluation of canine soft tissue neoplasms in veterinary pathology.
Gofflot, Stéphanie ; Université de Liège - ULiège > Département des sciences biomédicales et précliniques > Anatomie et cytologie pathologiques
Kischel, Philippe ; Université de Liège - ULiège > Département des sciences biomédicales et précliniques > Labo de recherche sur les métastases
Thielen, Caroline ; Université de Liège - ULiège > Département des sciences biomédicales et précliniques > Anatomie et cytologie pathologiques
Radermacher, Vincent ; Université de Liège - ULiège > Département des sciences biomédicales et précliniques > Anatomie et cytologie pathologiques
Boniver, Jacques ; Université de Liège - ULiège > Département des sciences biomédicales et précliniques > Anatomie et cytologie pathologiques
de Leval, Laurence ; Université de Liège - ULiège > Département des sciences biomédicales et précliniques > Anatomie et cytologie pathologiques - Département des sciences biomédicales et précliniques
Language :
English
Title :
Characterization of an antibody panel for immunohistochemical analysis of canine muscle cells
Bartunek J., Croissant J.D., Wijns W., Gofflot S., de Lavareille A., Vanderheyden M., Kaluzhny Y., Mazouz N., Willemsen P., Penicka M., Mathieu M., Homsy C., De Bruyne B., McEntee K., Lee I.W., and Heyndrickx G.R. Pretreatment of adult bone marrow mesenchymal stem cells with cardiomyogenic growth factors and repair of the chronically infarcted myocardium. Am. J. Physiol. Heart Circ. Physiol. 292 (2007) 1095-1104
Cooper B.J., Winand N.J., Stedman H., Valentine B.A., Hoffman E.P., Kunkel L.M., Scott M.O., Fischbeck K.H., Kornegay J.N., Avery R.J., Williams J.R., Schmickel R.D., and Sylvester J.E. The homologue of the Duchenne locus is defective in X-linked muscular dystrophy of dogs. Nature 334 (1988) 154-156
Cooper B.J. Animal models of Duchenne and Becker muscular dystrophy. Br. Med. Bull. 45 (1989) 703-718
Costa M.L., Escaleira R., Cataldo A., Oliveira F., and Mermelstein C.S. Desmin: molecular interactions and putative functions of the muscle intermediate filament protein. Braz. J. Med. Biol. Res. 37 (2004) 1819-1830
Dias P., Dilling M., and Houghton P. The molecular basis of skeletal muscle differentiation. Semin. Diagn. Pathol. 11 (1994) 3-14
Farah C.S., and Reinach F.C. The troponin complex and regulation of muscle contraction. FASEB J. 9 (1995) 755-767
Fishbein M.C., Wang T., Matijasevic M., Hong L., and Apple F.S. Myocardial tissue troponins T and I. An immunohistochemical study in experimental models of myocardial ischemia. Cardiovasc. Pathol. 12 (2003) 65-71
Grounds M.D., White J.D., Rosenthal N., and Bogoyevitch M.A. The role of stem cells in skeletal and cardiac muscle repair. J. Histochem. Cytochem. 50 (2002) 589-610
He K.L., Yi G.H., Sherman W., Zhou H., Zhang G.P., Gu A., Kao R., Haimes H.B., Harvey J., Roos E., White D., Taylor D.A., Wang J., and Burkhoff D. Autologous skeletal myoblast transplantation improved hemodynamics and left ventricular function in chronic heart failure dogs. J. Heart Lung Transpl. 24 (2005) 1940-1949
Lin Q., Schwarz J., Bucana C., and Olson E.N. Control of mouse cardiac morphogenesis and myogenesis by transcription factor MEF-2C. Science 276 (1997) 1404-1407
Linke A., Muller P., Nurzynska D., Casarsa C., Torella D., Nascimbene A., Castaldo C., Cascapera S., Bohm M., Quaini F., Urbanek K., Leri A., Hintze T.H., Kajstura J., and Anversa P. Stem cells in the dog heart are self-renewing, clonogenic, and multipotent and regenerate infarcted myocardium, improving cardiac function. Proc. Natl. Acad. Sci. U.S.A. 102 (2005) 8966-8971
Lints T.J., Parsons L.M., Hartley L., Lyons I., and Harvey R.P. Nkx-2.5: a novel murine homeobox gene expressed in early heart progenitor cells and their myogenic descendants. Development 119 (1993) 419-431
Miller J.B., Teal S.B., and Stockdale F.E. Evolutionarily conserved sequences of striated muscle myosin heavy chain isoforms. Epitope mapping by cDNA expression. J. Biol. Chem. 264 (1989) 13122-13130
Molkentin J.D. The zinc finger-containing transcription factors GATA-4, -5, and -6. Ubiquitously expressed regulators of tissue-specific gene expression. J. Biol. Chem. 275 (2000) 38949-38952
Pikkarainen S., Tokola H., Kerkela R., and Ruskoaho H. GATA transcription factors in the developing and adult heart. Cardiovasc. Res. 63 (2004) 196-207
Shiojima I., Komuro I., Mizuno T., Aikawa R., Akazawa H., Oka T., Yamazaki T., and Yazaki Y. Molecular cloning and characterization of human cardiac homeobox gene CSX1. Circ. Res. 79 (1996) 920-929
Silva G.V., Litovsky S., Assad J.A., Sousa A.L., Martin B.J., Vela D., Coulter S.C., Lin J., Ober J., Vaughn W.K., Branco R.V., Oliveira E.M., He R., Geng Y.J., Willerson J.T., and Perin E.C. Mesenchymal stem cells differentiate into an endothelial phenotype, enhance vascular density, and improve heart function in a canine chronic ischemia model. Circulation 111 (2005) 150-156
Skalli O., Ropraz P., Trzeciak A., Benzonana G., Gillessen D., and Gabbiani G. A monoclonal antibody against alpha-smooth muscle actin: a new probe for smooth muscle differentiation. J. Cell. Biol. 103 (1986) 2787-2796
Sobue K., and Sellers J.R. Caldesmon, a novel regulatory protein in smooth muscle and nonmuscle actomyosin systems. J. Biol. Chem. 266 (1991) 12115-12118
Valentine B.A., Cooper B.J., de Lahunta A., O'Quinn R., and Blue J.T. Canine X-linked muscular dystrophy. An animal model of Duchenne muscular dystrophy: clinical studies. J. Neurol. Sci. 88 (1988) 69-81
Vulliet P.R., Greeley M., Halloran S.M., MacDonald K.A., and Kittleson M.D. Intra-coronary arterial injection of mesenchymal stromal cells and microinfarction in dogs. Lancet 363 (2004) 783-784
Waltregny D., de Leval L., Glenisson W., Ly Tran S., North B.J., Bellahcene A., Weidle U., Verdin E., and Castronovo V. Expression of histone deacetylase 8, a class I histone deacetylase, is restricted to cells showing smooth muscle differentiation in normal human tissues. Am. J. Pathol. 165 (2004) 553-564
Weintraub H., Davis R., Tapscott S., Thayer M., Krause M., Benezra R., Blackwell T.K., Turner D., Rupp R., Hollenberg S., Zhuang Y., and Lassar A. The myoD gene family: nodal point during specification of the muscle cell lineage. Science 251 (1991) 761-766