Chitinase; Growth; Gut microbiota; Hermetia illucens; Oreochromis niloticus; Fatty Acids; Chitinases; Fatty Acids, Unsaturated; Animals; Larva; Animal Feed/analysis; Vegetables; Cichlids; Diptera/chemistry; Animal Feed; Diptera; Medicine (miscellaneous); Nutrition and Dietetics
Abstract :
[en] The aim of this study is to determine to what extent the addition of chitinase to black soldier fly (BSF) larval meal enriched or not with long-chain PUFA (LC-PUFA) could improve growth, protein digestion processes and gut microbial composition in Nile tilapia. Two different types of BSF meal were produced, in which larvae were reared on substrates formulated with vegetable culture substrate (VGS) or marine fish offal substrate (FOS). The BSF raised on VGS was enriched in α-linolenic acid (ALA), while that raised on FOS was enriched in ALA + EPA + DHA. Six BSF-based diets, enriched or not with chitinase, were formulated and compared with a control diet based on fishmeal and fish oil (FMFO). Two doses (D) of chitinase from Aspergillus niger (2 g and 5 g/kg feed) were added to the BSF larval diets (VGD0 and FOD0) to obtain four additional diets: VGD2, VGD5, FOD2 and FOD5. After 53 d of feeding, results showed that the BSF/FOS-based diets induced feed utilisation, protein efficiency and digestibility, as well as growth comparable to the FMFO control diet, but better than the BSF/VGS-based diets. The supplementation of chitinase to BSF/FOS increased in fish intestine the relative abundance of beneficial microbiota such as those of the Bacillaceae family. The results showed that LC-PUFA-enriched BSF meal associated with chitinase could be used as an effective alternative to fishmeal in order to improve protein digestion processes, beneficial microbiota and ultimately fish growth rate.
Disciplines :
Environmental sciences & ecology
Author, co-author :
Agbohessou, Pamphile S ; Research Unit in Environmental and Evolutionary Biology (URBE), Institute of Life, Earth and Environment (ILEE), University of Namur, Namur, Belgium ; Laboratory of Hydrobiology and Aquaculture (LHA), Faculty of Agronomics Sciences (FSA), University of Abomey-Calavi (UAC), Cotonou, Benin
Mandiki, Robert; Research Unit in Environmental and Evolutionary Biology (URBE), Institute of Life, Earth and Environment (ILEE), University of Namur, Namur, Belgium
Mes, Wouter; Department of Microbiology, Radboud Institute for Biological and Ecological Sciences (RIBES), Radboud University, Nijmegen, the Netherlands
Blanquer, Aude ; Université de Liège - ULiège > Fundamental and Applied Research for Animals and Health (FARAH) > FARAH: Santé publique vétérinaire
Gérardy, Mazarine ; Université de Liège - ULiège > Département de morphologie et pathologie (DMP) > Pathologie spéciale et autopsies
Garigliany, Mutien-Marie ; Université de Liège - ULiège > Département de morphologie et pathologie (DMP) > Pathologie générale et autopsies
Lambert, Jérôme; Research Unit in Environmental and Evolutionary Biology (URBE), Institute of Life, Earth and Environment (ILEE), University of Namur, Namur, Belgium
Cambier, Pierre; Unit of Research in Plant Cellular and Molecular Biology, Institute of Life, Earth and Environment (ILEE), University of Namur, Namur, Belgium
Tokpon, Nicole; Laboratory of Hydrobiology and Aquaculture (LHA), Faculty of Agronomics Sciences (FSA), University of Abomey-Calavi (UAC), Cotonou, Benin
Lalèyè, Philippe A; Laboratory of Hydrobiology and Aquaculture (LHA), Faculty of Agronomics Sciences (FSA), University of Abomey-Calavi (UAC), Cotonou, Benin
Kestemont, Patrick ; Université de Liège - ULiège > Département de gestion vétérinaire des Ressources Animales (DRA) ; Research Unit in Environmental and Evolutionary Biology (URBE), Institute of Life, Earth and Environment (ILEE), University of Namur, Namur, Belgium
Language :
English
Title :
Effect of fatty acid-enriched black soldier fly larvae meal combined with chitinase on the metabolic processes of Nile tilapia.
The authors thank the \u2018Academie de Recherche et d\u2019Enseignement Sup\u00E9rieur\u2019 (ARES \u2013 CCD, Belgium) for its financial support (accession numbers: 31382).
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