Realistic microplastics harness bacterial presence and promote impairments in early zebrafish embryos: Behavioral, developmental, and transcriptomic approaches. - 2024
Realistic microplastics harness bacterial presence and promote impairments in early zebrafish embryos: Behavioral, developmental, and transcriptomic approaches.
Bacterial colonization; Early life effect; Realistic microplastic; Zebrafish; Microplastics; Plastics; Water Pollutants, Chemical; Animals; Zebrafish/genetics; Plastics/metabolism; Embryo, Nonmammalian; Ecosystem; Gene Expression Profiling; Larva; Microplastics/toxicity; Microplastics/metabolism; Water Pollutants, Chemical/metabolism; Earliest life; Life stages; Transcriptomics; Zebrafish danio rerio; Zebrafish embryos; Environmental Engineering; Environmental Chemistry; Chemistry (all); Pollution; Public Health, Environmental and Occupational Health; Health, Toxicology and Mutagenesis
Abstract :
[en] The plastisphere is a newly recognized ecosystem. However, its interaction with early life stages of aquatic vertebrates is a multifaceted issue that requires further research. This study investigated the involvement of bacteria in shaping realistic microplastics hazards in zebrafish Danio rerio embryos. Fish were exposed to bottle micro-fragments (FR) and textile micro-fibers (FI) of polyethylene terephthalate (5-15 μm), concomitant with Aeromonas salmonicida achromogenes challenge from 2h post-fertilization for 3 days. Egg chorion showed affinity for FR and FI, inducing earlier embryo hatching. However, this effect was masked by biofilm invasion. Fragments were more detrimental than fibers on developmental parameters, while bacterial presence compromised body length, eye, and yolk sac surface area. In a further finding, MPs alone increased locomotor activity in zebrafish larvae, without synergistic effect when combined with bacteria. Data showed that realistic MPs had no significant effects except for downregulated sod and cyp1a gene expression, whereas bacterial challenge inhibited larval potency for most of the evaluated mRNA levels (mpx (immune system), apoeb (lipid metabolism), nfkb and tfa (inflammation), cyp and sod (oxidative stress)). This study provides new insights into realistic microplastic effects under relevant conditions when combined with environmental pathogen within the first life stages of aquatic vertebrates.
Disciplines :
Environmental sciences & ecology
Author, co-author :
Missawi, Omayma; University of Namur, Research Unit in Environmental and Evolutionary Biology (URBE), Institute of Life, Earth & Environment, Namur, Belgium. Electronic address: omayma.missawi@unamur.be
Wouters, Charlotte; University of Namur, Research Unit in Environmental and Evolutionary Biology (URBE), Institute of Life, Earth & Environment, Namur, Belgium
Lambert, Jérôme; University of Namur, Research Unit in Environmental and Evolutionary Biology (URBE), Institute of Life, Earth & Environment, Namur, Belgium
Garigliany, Mutien-Marie ; Université de Liège - ULiège > Département de morphologie et pathologie (DMP) > Pathologie générale et autopsies
Kestemont, Patrick ; Université de Liège - ULiège > Département de gestion vétérinaire des Ressources Animales (DRA) ; University of Namur, Research Unit in Environmental and Evolutionary Biology (URBE), Institute of Life, Earth & Environment, Namur, Belgium
Cornet, Valérie; University of Namur, Research Unit in Environmental and Evolutionary Biology (URBE), Institute of Life, Earth & Environment, Namur, Belgium
Language :
English
Title :
Realistic microplastics harness bacterial presence and promote impairments in early zebrafish embryos: Behavioral, developmental, and transcriptomic approaches.
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