[en] Bioluminescence is the production of visible light by living organisms. It occurs through the oxidation of luciferin substrates catalysed by luciferase enzymes. Auxiliary proteins, such as fluorescent proteins and luciferin-binding proteins, can modify the light emitted wavelength or stabilize reactive luciferin molecules, respectively. Additionally, calcium ions are crucial for the luminescence across various species. Despite the large phylogenetic distribution of bioluminescent organisms, only a few systems have been comprehensively studied. Notably, cnidarian species of the Renilla genus utilize a coelenterazine-dependent luciferase, a calcium-dependent coelenterazine-binding protein and a green fluorescent protein. We investigated the bioluminescence of three sea pen species: Pennatula phosphorea, Anthoptilum murrayi and Funiculina quadrangularis (Pennatuloidea, Anthozoa). Their light-emission spectra reveal peaks at 510, 513 and 485 nm, respectively. A coelenterazine-based reaction was demonstrated in all three species. Using transcriptome analyses, we identified transcripts coding for luciferases, green fluorescent proteins and coelenterazine-binding proteins for P. phosphorea and A. murrayi. Immunodetection confirmed the expression of luciferase in P. phosphorea and F. quadrangularis. We also expressed recombinant luciferase of A. murrayi, confirming its activity. We highlighted the role of calcium ions in bioluminescence, possibly associated with the mechanism of substrate release at the level of coelenterazine-binding proteins. The study proposes a model for anthozoan bioluminescence, offering new avenues for future ecological and functional research on these luminous organisms.
Duchatelet, Laurent ; Earth and Life Institute, Université catholique de Louvain, Louvain-la-Neuve, Walloon Brabant, Belgium
Galeazzo, Gabriela A; Departamento de Oceanografia Física, Química e Geológica, Universidade de São Paulo, Sao Paulo, Brazil
Coubris, Constance; Earth and Life Institute, Université catholique de Louvain, Louvain-la-Neuve, Walloon Brabant, Belgium
Bridoux, Laure; Louvain Institute of Biomolecular Science and Technology, Université catholique de Louvain, Louvain-la-Neuve, Walloon Brabant, Belgium
Rezsohazy, René; Louvain Institute of Biomolecular Science and Technology, Université catholique de Louvain, Louvain-la-Neuve, Walloon Brabant, Belgium
Melo, Marcelo R S; Departamento de Oceanografia Biológica, Universidade de São Paulo, Sao Paulo, Brazil
Marek, Milan; International Clinical Research Center, Masaryk University, Brno, Czech Republic
Amaral, Danilo T; Centre for Natural and Human Sciences, Universidade Federal do ABC, Santo Andre, Brazil
Dupont, Sam; Biological and Environmental Sciences, Goteborgs Universitet, Goteborg, Sweden ; International Atomic Energy Agency Marine Environment Laboratories, Monaco, Monaco
Garbuglio de Oliveira, Anderson; Department of Chemistry and Biochemistry, Yeshiva University, New York, NY, USA
Delroisse, Jérôme ; Université de Liège - ULiège > Département des sciences fonctionnelles (DSF) > Biochimie et biologie moléculaire ; Marine Organisms and Biomimetics, Université de Mons, Mons, Belgium
Language :
English
Title :
Insights into the bioluminescence systems of three sea pens (Cnidaria: Anthozoa): from de novo transcriptome analyses to biochemical assays.
H2020 - 730984 - ASSEMBLE Plus - Association of European Marine Biological Laboratories Expanded
Funders :
F.R.S.-FNRS - Belgian National Fund for Scientific Research FAPESP - Fundação de Amparo à Pesquisa do Estado de São Paulo Czech Science Foundation EU - European Union Czech Ministry of Education, Youth, and Sports CAPES - Coordenação de Aperfeicoamento de Pessoal de Nível Superior
Funding text :
This work was supported by an FRS-FNRS grant (T.0169.20) awarded to the Universit\u00E9 de Louvain\u2014UCLouvain Marine Biology Laboratory and the Universit\u00E9 de Mons Biology of Marine Organisms and Biomimetics Laboratory, by the Czech Science Foundation (GA22-09853S) and by the Czech Ministry of Education, Youth and Sports (RECETOX RI LM2023069, e-INFRALM2018140). The research leading to these results also received funding from the European Union\u2019s Horizon 2020 research and innovation programme under grant agreement no. 730984, ASSEMBLE Plus project. This study was financed in part by the Coordena\u00E7\u00E3o de Aperfei\u00E7oamento de Pessoal de N\u00EDvel Superior\u2014Brasil (CAPES)\u2014Finance Code 88887.605088/2021-00, Funda\u00E7\u00E3o de Amparo \u00E0 Pesquisa do Estado de S\u00E3o Paulo (FAPESP 2017/12909-4, 2020/07600-7) and Yeshiva University Start-up Fund. Acknowledgements
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