Comparative field efficacy of synthetic insecticides and plant extracts against Tuta absoluta (Lepidoptera: Gelechiidae) in Lubumbashi, DR Congo. - 2026
Solanum lycopersicum; Tuta absoluta; Botanicals; Integrated pest management; Synthetic insecticides
Abstract :
[en] The invasive tomato leafminer, Tuta absoluta (Meyrick), is a very destructive pest that poses a major threat to tomato (Solanum lycopersicum L.) production in the Democratic Republic of Congo (DRC), causing extensive yield losses through larval feeding on leaves, stems, and fruits. Chemical insecticides remain the primary control method, but resistance development, biodiversity loss and environmental concerns necessitate alternative strategies. This study aimed at evaluating the efficacy of synthetic and botanical insecticides against T. absoluta during two consecutive cropping seasons 2023-2024 in Lubumbashi, DRC, under both dry and rainy season conditions. Two tomato seedling varieties (Tanya F1 and Tovi Star F1) were treated with four synthetic insecticides (Dudu acelamectin 5% EC, cypermethrin 200 EC, lambda-cyhalothrin 50 EC and Occasion Star 200SC) and two botanical treatments (Tephrosia vogelii extract and Nimbecidine) at the recommended doses in a randomized complete block design. Pest incidence, larval density, leaf damage, and yield were assessed over multiple intervals. Results showed that synthetic insecticides, particularly Dudu acelamectin 5% EC, lambda-cyhalothrin 50 EC, and Occasion Star 200SC, significantly reduced T. absoluta larval infestations compared to cypermethrin 200 EC, which failed to control the pest due to suspected potential resistance. Botanical insecticides were also proved effective, with T. vogelii extract reducing leaf damage by 48% and Nimbecidine by 38%. The Tovi Star F1 variety exhibited inherent resistance, with lower pest incidence and higher yields than Tanya F1. Yield losses were strongly correlated with pest incidence and larval density, emphasizing the need for timely interventions. These findings highlight the potential of integrating synthetic and botanical insecticides with resistant tomato varieties for sustainable T. absoluta management in Lubumbashi. Future research should explore long-term resistance monitoring, cost-benefit analyses for smallholder farmers, and synergetic combinations of biopesticides to enhance efficacy while minimizing environmental impacts.
Disciplines :
Entomology & pest control
Author, co-author :
Mbuya, Sylvain Ntambo; Département de Phytotechnie, Faculté des Sciences Agronomiques, Université de Kolwezi, Kolwezi, Democratic Republic of the Congo. sylvain.mbuya@unikol.ac.cd ; Laboratoire de Recherche en Biofortification, Défense et Valorisation des Cultures (BioDev), Département de Production Végétale, Faculté des Sciences Agronomiques, Université de Lubumbashi, Lubumbashi, 1825, Democratic Republic of the Congo. sylvain.mbuya@unikol.ac.cd
Kankonda, Onesime M; Faculty for the Management of Natural Renewable Resources and Biodiversity Monitoring Center, University of Kisangani, Kisangani, Democratic Republic of the Congo
Fallah, Nyumah; School of Geography, Nanjing Normal University, Nanjing, 210023, China
Akutse, Komivi S; International Centre of Insect Physiology and Ecology (Icipe), Nairobi, Kenya ; Unit of Environmental Sciences and Management, North-West University, Private Bag X6001, Potchefstroom, 2520, South Africa
Cokola Cuma, Marcellin ; Université de Liège - ULiège > Département GxABT > Entomologie, Phytopathologie et Productions Innovantes (EPPI) ; Université de Liège - ULiège > TERRA Research Centre
Language :
English
Title :
Comparative field efficacy of synthetic insecticides and plant extracts against Tuta absoluta (Lepidoptera: Gelechiidae) in Lubumbashi, DR Congo.
Padmanabhan, P., Cheema, A. & Poliyath, G. Solanaceous fruits including tomato, eggplant, and peppers. In: Encyclopedia of Food and Health (eds. Caballero, B., Finglas, P.M. & Toldrá, F.). (Academic Press, 2016)
Hall RD Brouwer ID Fitzgerald MA Plant metabolomics and its potential application for human nutrition Physiol. Plant. 2008 132 162 175 18251858
Akutse KS et al. Entomopathogenic fungus isolates for adult Tuta absoluta (Lepidoptera: Gelechiidae) management and their compatibility with Tuta pheromone J. Appl. Entomol. 2020 144 777 787
Muhorakeye MC et al. Biostimulant and antagonistic potential endophytic fungi against furasarium wilt pathogen of tomato Fusarium oxysporum f. sp. lycopersici Sci. Rep. 2024 14 1536
ReportLinker. Tomato industry outlook in the Democratic Republic of the Congo, 2022–2026. Available at: https://www.reportlinker.com/clp/country/484797/726330 (2025).
Mukwa LFT et al. First report of the South American tomato pinworm Tutaabsoluta (Meyrick) (Lepidoptera: Gelechiidae) and its damage in the Democratic Republic of Congo BioInvasions Rec. 2021 10 33 44
Tonnang HE Mohamed SA Khamis F Ekesi S Identification and risk assessment for worldwide invasion and spread of Tutaabsoluta with a focus on Sub-Saharan Africa: Implication for phytosanitary measures and management PLoS ONE 2015 10 26360584 4567337 e0138319
Mansour R et al. Occurrence, biology, natural enemies and management of Tutaabsoluta in Africa Entomol. Gen. 2018 38 83 112
Han P et al. Bottom-up effects of irrigation, fertilization and plant resistance on Tutaabsoluta: Implications for integrated pest management J. Pest Sci. 2019 92 1359 1370 2019aosl.book...H
Kandil MA et al. Comparative toxicity of new insecticides generations against tomato leaf miner Tutaabsoluta and their biochemical effects on tomato plants Bull. Natl. Res. Cent. 2020 44 126
Desneux N et al. Biological invasion of European tomato crops by Tutaabsoluta: Ecology, geographic expansion and prospects for biological control J. Pest Sci. 2010 83 197 215
Garzia GT Siscaro G Biondi A Zappalà L Tuta asboluta, a South American pest of tomato now in the EPPO region: Biology, distribution and damage EPPO Bull. 2012 42 205 210
Brévault T et al. Tuta absoluta Meyrick (Lepidoptera: Gelechiidae): A new threat to tomato production in Sub-Saharan Africa Afr. Entomol. 2014 22 441 444
Desneux N Luna MG Guillemaud T Urbaneja. A The invasive American tomato pinworm, Tutaabsoluta, continues to spread in Afro-Eurasia and beyond: The new threat to tomato world production J. Pest Sci. 2011 84 403 408
Mawcha, K.T. et al. An overview of sustainable management strategies for Tuta absoluta. Int. J. Pest Manag. 1–24 (2025).
Biondi A et al. Ecology, world-wide spread, and management of the invasive South American tomato pinworm, Tutaabsoluta: Past, present, and future Annu. Rev. Entomol. 2018 63 239 258 28977774
Ahmed SS et al. Control of Tutaabsoluta (Lepidoptera: Gelechiidae) by the new trend of photosensitizer and nanocomposites and their effects on productivity and storability of tomato Int. J. Trop. Insect Sci. 2024 44 273 296
Guedes RNC Picanҫo MC The tomato borer Tutaabsoluta in South America: Pest status, management and insecticide resistance EPPO Bull. 2012 42 211 216
Rostami E et al. Pest density influence on tomato pigment contents: The South American tomato pinworm scenario Entomol. Gen. 2020 40 195 205
Kaoud HA Alternative methods for the control of Tutaabsoluta GJMAS 2014 2 41 46
Simmons, A.M. et al. “Lepidopterous pests: Biology, Ecology, and Management.”. In: Sustainable Management of Arthropod Pests of Tomato (eds. Wakil, W., Brust, G.E. & Perring, T.) 131–162. (Oxford Academic Press, 2018)
IRAC. Tuta absoluta on the move. Available at: http://www.irac-online.org/documents/eConnection_issue20a.pdf (2009)
Guedes RNC et al. Insecticide resistance in the tomato pinworm Tuta absoluta: Patterns, spread, mechanisms, management and outbreak J. Pest Sci. 2019 92 1329 1342
Rwomushana, I. et al. Evidence Note. Tomato leafminer (Tuta absoluta): impacts and coping strategies for Africa. CABI Working paper 12, 56 (2019).
Campos MR et al. Susceptibility of Tuta absoluta(Lepidoptera: Gelechiidae) Brazilian populations to ryanodine receptor modulators Pest Manag. Sci. 2015 71 537 544 24863675
Roditakis E et al. A four-year survey on insecticide resistance and likelihood of chemical control failure for tomato leaf miner Tuta absoluta in the European/Asia region J. Pest Sci. 2018 91 421 435
Agbessenou A et al. Endophytic fungi protect tomato and nightshade plants against Tuta absoluta(Lepidoptera: Gelechiidae) through a hidden friendship and cryptic battle Sci. Rep. 2020 10 2020NatSR.1022195A 33335143 7746743 22195
Tarusikirwa VL et al. Tuta absoluta(Meyrick) (Lepidoptera: Gelechiidae) on the “Offensive” in Africa: Prospects for integrated Pest management initiatives Insects 2020 11 764 33171892 7694550
Peel MC Finlayson BL McMahon TA Updated world map of the Köppen-Geiger climate classification Hydrol. Earth Syst. Sci. 2007 11 1633 1644 2007HESS..11.1633P
Assani AA Analyse de la variabilité temporelle des précipitations (1916-1996) à Lubumbashi (Congo-Kinshasa) en relation avec certains indicateurs de la circulation atmosphériques (oscillation australe) et océanique (El Niño/La Niña) Sécheresse 1999 10 245 252
Stevenson PC et al. Distinct chemotypes of Tephrosia vogelii and implications for their use in pest control and soil enrichment Phytochemistry 2012 78 135 146 22483325
Gaskins, M.H. Tephrosia vogelii: a source of rotenoids for insecticidal and pesticidal use. Technical Bulletin 1445: 1–38 (USDA,1972)
Stevenson PC Belmain SR Pesticidal plants in African agriculture: Local uses and global perspective Pestic. Outlook 2016 10 226 229
Henry MC Yonker CR Supercritical fluid chromatography. Pressurized liquid extraction, and supercritical fluid extraction Anal. Chem. 2006 78 3909 3916 2006AnaCh.78.3909H 16771531
Nderevimana A et al. Field efficacy of entomolopathogens and plant extracts on Tuta absoluta Meyrick (Lepidoptera: Gelechiidae) infesting tomato in Rwanda Crop Prot. 2020 134 105183
Onunkun O Evaluation of aqueous extracts of five plants in the control of flea beetles on okra J. Biopestic. 2012 5 62 67
Gözel Ҫ Kasap I Efficacy of entomopathogenic nematodes against the tomato leafminer, Tutaabsoluta (Meyrick) (Lepidoptera: Gelechiidae) in tomato field Turk. J. Entomol. 2015 39 229 237
Ahmad MF et al. Pesticides impacts on human health and the environment with their mechanisms of action and possible countermeasures Heliyon 2024 10 38623208 11016626 e29128
Cocco A Deliperi S Delrio G Control of Tutaabsoluta (Meyrick) (Lepidoptera: Gelechiidae) in greenhouse tomato crops using mating disruption technique J. Appl. Entomol. 2013 137 16 28
Liu J et al. Tomato yield and water use efficiency change with various soil moisture and potassium levels during different growth stages PLoS ONE 2019 14 eo210206
Ali A et al. Evaluation of various tomato (Lycopersicon esculentum Mill) cultivars for quality, yield and yield components under agroclimatic condition of Peshawar ARPN J. Agric. Biol. Sci. 2016 11 59 62
R Core Team R: A Language and Environment for Statistical Computing 2024 R Foundation for Statistical Computing
Shapiro SS Wilk MB An analysis of variance test for normality (complete samples) Biometrika 1965 52 591 611 205384
Elkin, L.A. et al. An Aligned Rank Transform Procedure for Multifactor Contrast Tests. In: The 34th Annual ACM Symposium on User Interface Software and Technology (UIST ‘21). Association for Computing Machinery, New York, NY, USA, 754–768 (2021).
Harrison XA et al. A brief introduction to mixed effects modelling and multi-model inference in ecology PeerJ 2018 6 29844961 5970551 e4794
Wickham, H. ggplot2: Elegant graphics for data analysis. New York, NY: Springer New York 260 (2009).
Cokola MC et al. First report of Spodoptera frugiperda(Lepidoptera: Noctuidae) on onion (Allium cepa L.) in South Kivu, Eastern DR Congo Rev. Bras. Entomol. 2021 65 e20200083.2021
Ntambo MS et al. Occurrence of the sweet potato hornworm Agrius convolvuli(Lepidoptera: Sphingidae) in Haut-Katanga province, Democratic Republic of the Congo J. Entomol. Acarol. Res. 2022 54 10424
Rafiki Pest Control. Dudu acelamectin Tuta absoluta insecticide 5%-500ml. Available at: https://shop.rafikipestcontrol.com/products/dudu-acelamectin-tatu-absoluta-insecticide-5-500ml (2025).
Kabaale FP et al. First report of field efficacy and economic viability of Metarhizium anisopliae- ICIPE 20 for Tuta absoluta(Lepidoptera: Gelechiidae) management on tomato Sustainability 2022 14 2022Sust..1414846K 14846
Zhang H et al. Lambda-cyhalothrin induces heart injury in chickens by regulating cytochrome P450 enzyme system and inhibiting Nrf2/HO-1 pathway Poult. Sci. 2024 103 39137500 11372963 104154
Keyhanian AA Barari H Mobasheri MT Comparison of the efficacy of insecticides, alphacypermethrin and lambda-cyhalothrin, against canola flea beetles Appl. Entomol. Phytopathol. 2021 44 113 122
Li JY et al. Sublethal effects of lambda-cyhalothrin on the biological characteristics, detoxification enzymes, and genes of the papaya mealybug Paracoccus marginatus. J. Pest Sci. 2025 98 783 797
Mahmoud YA et al. Effect of certain low toxicity insecticides against tomato leaf miner (Tuta absoluta) with reference to their residues in harvested tomato fruits Int. J. Agric. Res. 2014 9 210 218
El-Ghany NMA Abdel-Razek AS Abadah IMA Mahmoud YA Evaluation of some microbial agents, natural and chemical compounds for controlling tomato leaf miner, Tutaabsoluta (Meyrick) (Lepidoptera: Gelechiidae) J. Plant Prot. Res. 2016 56 373 379
Khan G et al. Assessment of different synthetic insecticides for the management of tomato leaf miner (Tuta abosluta) J. Xi’an ShiyouUniv. (Nat. Sci. Ed) 2023 19 47 57
Haddi K et al. Identification of mutations associated with pyrethroid resistance in the voltage-gated sodium channel of the tomato leaf miner (Tuta absoluta) Insect Biochem. Mol. Biol. 2012 42 506 513 22504519
Silva WM et al. Status of pyrethroid resistance and mechanisms in Brazilian populations ofTutaabsoluta Pestic. Biochem. Physiol. 2015 122 8 14 26071801
Biondi A et al. Potential toxicity of α-Cypermethrin-treated nets on Tuta absoluta(Lepidoptera: Gelechiidae) J. Econ. Entomol. 2015 108 1191 1197 26470245
Siqueira HAA Guedes RNC Picanco MC Cartap resistance and synergism in population of Tutaabsoluta (Lep., Gelechiidae) J. Appl. Entomol. 2000 124 233 238
Siqueira HAA Guedes RNC Fragoso DB Magalhães LC Abamectin resistance and synergism in Brazilian populations of Tutaabsoluta (Meyrick) (Lepidootera: Gelechiidae) Int. J. Pest Manag. 2001 47 247 251
Silva WM et al. Mutation (G275E) of the nicotinic acetylcholine receptor α6 subunit is associated with high levels of resistance to spinosyns in Tuta absoluta(Meyrick) (Lepidoptera: Gelechiidae) Pestic. Biochem. Physiol. 2016 131 1 8 2016prob.book...S 27265820
Roditakis E et al. Ryanodine receptor point mutations confer diamide insecticide resistance in tomato leafminer, Tuta absoluta(Lepidoptera: Gelechiidae) Insect Biochem. Mol. Biol. 2017 80 11 20 27845250
Sparks TC Nauen R IRAC: Mode of action classification and insecticide resistance management Pestic. Biochem. Physiol. 2015 121 122 128 26047120
Greenlife. Occasion Star 200SC. Available at: https://www.greenlife.co.ke/product/occasion-star-200sc/ (2025).
Lapied B Grolleau F Sattelle DB Indoxacarb, an oxadiazine insecticide, blocks insect neuronal sodium channels Br. J. Pharmacol. 2001 132 587 595 11159709 1572588
Bengochea P et al. Is emamectin benzoate effective against the different stages of Spodoptera exigua(Hübner) (Lepidoptera, Noctuidae)? Ir. J. Agric. Food Res. 2014 53 37 49
Roditakis E Skarmoutsou C Staurakaki M Toxicity of insecticides to populations of tomato borer Tutaabsoluta (Meyrick) from Greece Pest Manag. Sci. 2013 69 834 840 23757288
Roby AE Hussein S Behavior of bio-and chemical insecticides in tomato ecosystem in Minia Governorate Acta Ecol. Sin. 2019 39 152 159
López JD Jr Latheef MA Hoffman WC Effect of emamectin benzoate on mortality, proboscis extension, gustation and reproduction of the corn earworm, Helicoverpa zea J. Insect Sci. 2010 10 20673074 3383429 89
Talib A Hamdi H Abd al-Rahman SM Sherby SM Efficacy of some natural oils on the residual toxicity of emamectin benzoate, spinosad and spinetoram against Egyptian cotton leafworm J. Pest. Control. Envrion. Sci. 2008 16 37 56
Popowski J et al. Glandular trichome rupture in tomato plants is an ultra-fast and sensitive defense mechanism against insects J. Exp. Bot. 2025 76 6508 6519 40491092 12646162
Bar M Shtein I Plant trichomes and the biomechanics of defense in various systems, with Solanaceae as a model Botany 2019 97 651 660
Bleeker PM et al. Improved herbivore resistance in cultivated tomato with the sesquiterpene biosynthetic pathway from a wilt relative Proc. Natl. Acad. Sci. U. S. A. 2012 109 20124 20129 2012PNAS.10920124B 23169639 3523864
Syngenta. Tovi Star F1. Available at: https://www.syngenta.co.zm/product/seed/tomatoes/tovi-star (2025).
Semena. Tomatoes Tanya F1. Available at: http://semenaopt.com/en/Tomatoes/Tanya_F1/602904/ (2025).
Lazo CR Guillot TS Miller GW Aminoff MJ Daroff RB Rotenone Encyclopedia of the Neurologcal Sciences 2014 2nd Academic Press 74 75
Kayange CDM Njera D Nyirenda SP Mwamlima L Effectiveness of Tephrosia vogelii and Tephrosia candida extract against common ben aphid (Aphid fabae) in Malawi Adv. Agric. 2019 2019 6704834
Stevenson PC Isman MB Belmain SR Pesticidal plants in Africa: A global vision of new biological control products from local uses Ind. Crops Prod. 2017 110 2 9
Nilahyane A Bouharroud R Hormatallah A Taadaouit A Larvicidal effect of plant extract on Tutaabsoluta (Lepidoptera: Gelechiidae). Working group integrated control in protected crops Mediterranean climate IOBC-WPRS Bull. 2012 80 305 310
Ochieng TA et al. Interactions betweenBacillus thuringiensis and selected plant extracts for sustainable management ofPhthorimaea absoluta Sci. Rep. 2024 14 2024NatSR.14.9299O 38653843 11039665 9299
Aynalem B Empirical review of Tutaabsoluta Meyrick effect on the tomato production and their protection attempts Adv. Agric. 2022 2022 2595470
Saleem U et al. Determination of insecticidal potential of selected plant extracts against fall armyworm (Spodoptera frugiperda) larvae Heliyon 2024 10 39498049 11532869 e39593
Kona NEM Taha AK Mahmoud MEE Effects of botanical extracts of neem (Azadirachta indica) and Jatropha (Jatropha curcus) on eggs and larvae of tomato leaf miner, Tutaabsoluta (Meyrick) (Lepidoptera: Gelechiidae) PGCP 2014 3 41 46
Kamanula J et al. Farmers’insect pest management practices and pesticidal plant use in the protection of stored maize and bean in Southern Africa Int. J. Pest Manag. 2011 57 41 49
Mafongoya PL Kuntashula E Participatory evaluation of Tephrosia species and provenances for soil fertility improvement and other uses using farmer’s criteria in eastern Zambia Exp. Agric. 2005 41 69 80
Cabello T et al. Selection of Trichogramma spp. (Hymenoptera: Trichogrammatidae) for biological control of Tuta abosulta(Lepidoptera: Gelechiidae) in greenhouses by an entomo-ecological simulation model IOBC-WPRS Bull. 2012 80 171 176
Sisay B et al. The efficacy of selected synthetic insecticides and botanicals against Tuta abosulta (Meyrick) under laboratory and field conditions Crop Prot. 2018 110 202 208
Kenis M et al. Telenomus remus, a candidate parasitoid for the biological control of Spodoptera frugiperda in Africa, is already present on the continent Insects 2019 10 92 30934941 6523282
Lisi F et al. Non-target effects of bioinsecticides on natural enemies of arthropod pests Curr. Opin. Environ. Sci. Health 2025 45 100624
IRAC. Tuta absoluta - the Tomato leaf miner or Tomato Borer. Recommendations for sustainable and Effective Resistance Management. Available at: https://irac-online.org/content/uploads/2009/12/Tuta_brochure_print-version_11Oct11.pdf (2011).
Balasha AM Nsele MK Pesticide use practices by Chinese cabbage growers in suburban environment of Lubumbashi (DR Congo): Main pests, costs and risks JAAEPA 2019 2 56 64
Lietti MMM Botto E Alzogaray RA Insecticide resistance in Argentine populations of Tutaabsoluta (Meyrick) (Lepidoptera: Gelechiidae) Neotrop. Entomol. 2005 34 113 119
Domínguez A et al. Influence of age, host plant and mating status in pheromone production and new insights on perception plasticity in Tutaabsoluta Insects 2019 10 256 31434304 6722863
Campos MR et al. Spinosad and the Tomato Borer Tutaabsoluta: A bioinsecticide, an invasive pest threat, and high insecticide resistance PLoS ONE 2014 9 2014PLoSO..9j3235C 25122089 4133407 e103235
Sylla S Seydi O Diarra K Brevault T Seasonal decline of the tomato leafminer, Tutaabsoluta, in the shifting landscape of a vegetative-growing area Entomol. Exp. Appl. 2018 166 638 647
Sylla S et al. Seasonal abundance and role of host plant on the tomato leaf miner populations dynamics, Tutaabsoluta Senegal. Acta Hortic. 2022 1348 1 8
Subedi B Poudel A Aryal B The impact of climate change on insect pest biology and ecology: Implications for pest management strategies, crop production, and food security J. Agric. Food Res. 2023 14 100733
Bacci L et al. The seasonal dynamic of Tutaabsoluta in Solanum lycopersicon cultivation: Contributions of climate, plant phenology, and insecticide spraying Pest Manag. Sci. 2021 77 3187 3197 33683006
Cokola MC et al. Planting data in South Kivu, eastern DR Congo: A real challenge for the sustainable management of Spodoptera frugiperda(Lepidoptera: Noctuidae) by smallholder farmers PLoS ONE 2024 19 39621599 11611118 e0314615
Niassy S et al. An African perspective for harmonized policies framework on plant protection products (PPPs) AFJRD 2025 10 2025
Retta A Berhe D Tomato leafminer–Tutaabsoluta (Meyrick), a devastating pest of tomatoes in the highlands of Northern Ethiopia: A call for attention and action RJAES 2015 4 264 269
