[en] To better understand the effect of habituation on gene expression in plant cells, we have compared the accumulation of specific mRNAs encoding respectively two proline-rich proteins, a chaperone protein and three enzymes linking primary and secondary metabolisms in two models of in vitro culture of periwinkle. These models consisted of two couples of a 2,4-dichlorophenoxyacetic acid-dependent/2,4-dichlorophenoxyacetic acid independent line in which autonomy to auxin and cytokinin was obtained either through habituation or through transformation with the isopentenyltransferase gene from Agrobacterium tumefaciens. Results showed that gene expression was modified by plant growth regulator autonomy but differently according to the type of autonomy: only the gene encoding a hydroxyproline-rich glycoprotein was regulated similarly in both PGR-independent lines. On the other hand, PGR autonomy did not lead to total insensitivity to exogenously-applied PGRs, and the two PGR autonomous lines did not accumulate indole alkaloids for different reasons.
S.E.I. Beinsberger R.L.M. Walcke R.Y. Deblaere H.M.M. Clijsters J.A. de Greef H.I. van Onckelen Effects of the introduction of Agrobacterium tumefaciens T-DNA ipt gene in Nicotiana tabacum L. cv. Petit Havana SRI plant cells Plant Cell Physiol. 32 1991 489 496
B.R. Campell Y.L. Su W.L. Pengelly Auxin autonomy in cultured tobacco teratoma tissues transformed by an auxin-mutant strain of Agrobacterium tumefaciens Planta 188 1992 123 128
B.R. Campell C.D. Town Characterization of overexpressed cDNAs isolated from a hormone-autonomous, radiation-induced tumor tissue line of Arabidopsis thaliana Plant Physiol. 100 1992 2018 2023
P. Christou Habituation in in vitro soybean cultures Plant Physiol. 88 1988 809 812
M. Clastre H. Maaroufi F. Andreu J.C. Chenieux M. Rideau S. Hamdi Isolation of a full-length cDNA encoding a cytosolic cyclophilin from periwinkle ( Catharanthus roseus ) Plant Physiol. 109 1995 1497
D.R. Corbin N. Sauer C. Lamb Differential regulation of an hydroxyproline-rich glycoprotein gene family in wounding and infected plants Mol. Cell. Biol. 7 1987 4337 4344
V. De Luca C. Marineau N. Brisson Molecular cloning and analysis of cDNA encoding a plant tryptophan decarboxylase: comparison with animal dopa decarboxylases Proc. Nad. Acad. Sci. USA 86 1989 2582 2586
A.M. Droual H. Maaroufi J. Creche J.C. Chenieux M. Rideau S. Hamdi Changes in the accumulation of cytosolic cyclophilin transcripts in cultured periwinkle cells following hormonal and stress treatments J. Plant Physiol. 151 1997 142 150
S. Du Plessis W.A. Stirk W.A. Cress J. van Staden Biochemical comparisons of habituated and non-habituated callus lines of Glycine max (L.) cv Acme. Plant Growth Reg. 18 1996 223 231
S. Eklöf C. Ästot J. Blackwell Th. Moritz O. Olsson G. Sandberg Auxin-cytokinin interactions in wild-type and transgenic tobacco Plant Cell Physiol. 38 1997 225 235
S. Feutry D. Poder D. Hagege Enhancement of diacylglycerol level and inositol phosphates turnover in fully-habituated sugar beet cell line Plant Physiol. Biochem. 33 1995 115 120
O.L. Gamborg R.A. Miller K. Ojima Nutrient requirements of suspension cultures of soybean root cells Exp. Cell Res. 50 1968 151 158
F. Garnier S. Carpin P. Label J. Creche M. Rideau S. Hamdi Effect of cytokinin on alkaloid accumulation in periwinkle callus cultures transformed with a light-inducible ipt gene Plant Science 120 1996 47 55
Th. Gaspar The concept of cancer in in vitro plant cultures and the implication of habituation to hormones and hyperhydricity Plant Tissue Culture Biotechnol. 1 1995 126 136
Th. Gaspar D. Hagege C. Kevers C. Penel M. Crevecoeur I. Engelmann H. Greppin J.M. Foidart When plant teratomas turn into cancers in the absence of pathogens Physiol. Plant. 83 1991 696 701
R.J. Gautheret Sur la variabilité des propriétés physiologiques des cultures de tissus de plantes Rev. Gen. Bot. 62 1995 1 106
M.J. Gething J. Sambrook Protein folding in the cell Nature 355 1992 33 44
O.J.M. Goddijn R.J. de Kam A. Zanetti R.A. Schilperoort J.H.C. Höge Auxin rapidly down-regulates transcription of the tryptophan decarboxylase gene from Catharanthus roseus Plant Mol. Biol. 18 1992 1113 1120
O.J.M. Goddijn E.J.M. Pennings P. van der Helm R.A. Schilperoort R. Verpoorte J.H.C. Hoge Overexpression of a tryptophan decarboxylase cDNA in Catharanthus roseus crown-gall calluses results in increased tryptamine levels but not in increased terpenoid indole alkaloid production Transg. Res. 4 1995 315 323
L. Herrera-Estrella G. van den Broeck R. Maenhaut M. van Montagu J. Schell M. Timko A. Cashmore Light-inducible and chloroplast-associated expression of a chimaeric gene introduced into Nicotiana tabacum using a Ti plasmid vector Nature 310 1984 115 120
M. Hotze A. Waitz J. Schröder A cDNA clone for a 14-kilodalton polypeptide from Madagascar periwinkle ( Catharanthus roseus ). Eight conserved cysteines in six related proteins from different plants suggest common functional elements Plant Physiol. 104 1994 1097 1098
A. Jemmali P. Boxus C. Kevers Th. Gaspar Carry-over of morphological and biochemical characteristics associated to hyperflowering of micropropagated strawberries J. Plant Physiol. 147 1995 435 440
M. José P. Puigdomenech Structure and expression of genes coding for structural proteins of the plant cell wall New Phytol. 125 1993 259 282
C. Kevers M. Filali G. Petit-Paly D. Hagege M. Rideau Th. Gaspar Habituation of plant cells does not mean insensitivity to plant growth regulators. In Vitro Cell Dev Biol. Plant 32 1996 204 209
A.J. Koletsky M.W. Harding R.E. Handschumacher Cyclophilins: Distribution and variant properties in normal and neoplastic tissues J. Immunol. 137 1986 1054 1059
S.D. Kung Role of cytokinin in Nicotiana genetic tumors. A working hypothesis Physiol. Plant. 82 1991 474 476
P. Lambé H.S.N. Mutambel J.G. Fouche R. Deltour J.M. Foidart Th. Gaspar DNA methylation as a key process in regulation of organogenic totipotency and plant neoplastic progression In Vitro Cell. Dev. Biol. Plant 33 1997 152 162
I.E. Maldonado-Mendoza R.J. Burnett C.L. Nessler Nucleotide sequence of a cDNA encoding 3-hydroxy-3-methylglutaryl coenzyme A reductase from Catharanthus roseus Plant Physiol. 100 1992 1613 1614
H. Masuda R. Nakagawa S. Sugawara Hormone-autonomous suspension culture from leaf expiants of sugar beets in liquid medium Plant Cell Physiol. 29 1988 75 78
J.M. Mérillon M. Filali P. Duperon M. Montagu J.C. Chenieux M. Rideau Effect of 2,4-dichlorophenoxyacetic acid and habituation on lipid and protein composition of microsomal membranes from periwinkle cell suspensions Plant Physiol. Biochem. 33 1995 443 451
J.M. Mérillon L. Ouelhazi P. Doireau J.C. Chenieux M. Rideau Metabolic changes and alkaloid production in habituated and non-habituated cells of Catharanthus roseus grown in hormone-free medium. Comparing hormone-deprived non-habituated cells with habituated cells J. Plant Physiol. 134 1989 54 60
J.M. Mérillon K. Ramawat F. Andreu J.C. Chenieux M. Rideau Accumulation alcaloïdique dans des lignées cellulaires de C atharanthus roseus entretenues en préesence ou en absence de phytohormones C.R. Acad. Sci. Paris 303 III 1986 689 692
S.M. Persinger C.D. Town Isolation and characterization of hormone-autonomous tumours of Arabidopsis thaliana J. Exp. Bot. 42 1991 1363 1370
G. Schröder J. Eichel S. Breinig J. Schröder Three differentially expressed S-adenosylmethionine synthetases from Catharanthus roseus: molecular and functional characterization Plant Mol. Biol. 33 1997 211 222
H.H. Smith Plant genetic tumours Progress in Experimental Tumour Research 15 1972 138 164
R. Soni J.P. Carmichael Z.H. Shah J.A.H. Murray A family of cydin D homologs from plants differentially controlled by growth regulators and containing the conserved retinoblastoma protein interaction motif Plant Cell 7 1995 85 103
P. Tacchini A. Fink G.X. Xue Th. Gaspar H. Greppin Analysis of proteins of a fully-habituated nonorganogenic sugar-beet callus and a hormone-dependent one by high-performance 2D-gel electrophoresis Plant Physiol. Biochem. 33 1995 361 366
T.C. Wang R.D. Cardiff L. Zukerbery E. Lees A. Arnold E.V. Schmidt Mammary hyperplasia and carcinoma in MMTV-cyclin D1 transgenic mice Nature 369 1994 669 671
H.N. Wood A. G Braun Studies on the net uptake of solutes by normal and crown-gall tumor cells Proc. Nad. Acad. Sci. USA 54 1965 1532 1538
