With No-Lysine Kinase 1 Regulates Biotic and Abiotic Stress Tolerance in Rice by Targeting OsCATA to Alter H2O2 Homeostasis.

Zhang, Yue; Liu, Qunen; Kang, Yiwei; Wang, Guanqi; Duan, Wenjing; Zhang, Yingxin; Wu, Weixun; Chen, Daibo; Hong, Yongbo; Sun, Lianping; Shen, Xihong; Zhan, Xiaodeng; Cheng, Shihua; Ludivine, Lassois; Cao, Liyong

doi:10.1111/mpp.70256

Article (Scientific journals)

With No-Lysine Kinase 1 Regulates Biotic and Abiotic Stress Tolerance in Rice by Targeting OsCATA to Alter H2O2 Homeostasis.

Zhang, Yue; Liu, Qunen; Kang, Yiwei et al.

2026 • In Molecular Plant Pathology, 27 (4), p. 70256

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https://hdl.handle.net/2268/344632

DOI
10.1111/mpp.70256

PubMed
41943442

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Keywords :

catalase; reactive oxygen species; receptor‐like cytoplasmic kinases; rice; with no‐lysine kinase; Plant Proteins; Hydrogen Peroxide; Reactive Oxygen Species; Homeostasis; Reactive Oxygen Species/metabolism; Gene Expression Regulation, Plant; Plant Diseases/microbiology; Disease Resistance/genetics; Droughts; Oryza/genetics; Oryza/physiology; Oryza/metabolism; Oryza/enzymology; Oryza/microbiology; Plant Proteins/metabolism; Plant Proteins/genetics; Stress, Physiological/genetics; Hydrogen Peroxide/metabolism; Disease Resistance; Oryza; Plant Diseases; Stress, Physiological; Molecular Biology; Agronomy and Crop Science; Soil Science; Plant Science

Abstract :

[en] Receptor-like cytoplasmic kinases (RLCKs) play pivotal roles in regulating plant responses to both biotic and abiotic stresses. Although numerous RLCKs have been identified, their underlying regulatory mechanisms remain incompletely understood. In our previous work, we identified the rice OsRLCK109 as a negative regulator of blast resistance. In this study, we aimed to identify interaction partners of OsRLCK109 and to investigate their role in rice responses to both biotic and abiotic stresses. We identified OsWNK1 as an interacting partner of OsRLCK109 and characterised its inhibitory role in regulating rice blast disease, as well as drought and cold stress responses. OsWNK1 directly phosphorylates OsCATA, modulating its enzymatic activity and leading to elevated reactive oxygen species (ROS) levels during pathogen infection. This enhances immune signalling and improves disease resistance. However, under abiotic stress conditions, reduced catalase activity in oswnk1 mutants impairs ROS scavenging, resulting in ROS toxicity and decreased stress tolerance. These findings reveal the dual regulatory role of the OsRLCK109-OsWNK1-OsCATA module in both biotic and abiotic stress responses, thereby offering new insights into a more comprehensive understanding of the stress response mechanism in rice through ROS signalling.

Disciplines :

Agriculture & agronomy

Author, co-author :

Zhang, Yue ; 列日大学 - ULiège > TERRA研究中心

Liu, Qunen; State Key Laboratory of Rice Biology and Breeding, China National Rice Research Institute, Hangzhou, China

Kang, Yiwei; State Key Laboratory of Rice Biology and Breeding, China National Rice Research Institute, Hangzhou, China ; National Key Laboratory of Crop Genetic Improvement and National Center of Plant Gene Research, Huazhong Agricultural University, Wuhan, Hubei, China

Wang, Guanqi; State Key Laboratory of Rice Biology and Breeding, China National Rice Research Institute, Hangzhou, China

Duan, Wenjing; State Key Laboratory of Rice Biology and Breeding, China National Rice Research Institute, Hangzhou, China

Zhang, Yingxin; State Key Laboratory of Rice Biology and Breeding, China National Rice Research Institute, Hangzhou, China

Wu, Weixun; State Key Laboratory of Rice Biology and Breeding, China National Rice Research Institute, Hangzhou, China

Chen, Daibo; State Key Laboratory of Rice Biology and Breeding, China National Rice Research Institute, Hangzhou, China

Hong, Yongbo; State Key Laboratory of Rice Biology and Breeding, China National Rice Research Institute, Hangzhou, China

Sun, Lianping; State Key Laboratory of Rice Biology and Breeding, China National Rice Research Institute, Hangzhou, China

More authors (5 more)

Language :

English

Title :

With No-Lysine Kinase 1 Regulates Biotic and Abiotic Stress Tolerance in Rice by Targeting OsCATA to Alter H2O2 Homeostasis.

Publication date :

April 2026

Journal title :

Molecular Plant Pathology

ISSN :

1464-6722

eISSN :

1364-3703

Publisher :

John Wiley and Sons Inc

Volume :

Issue :

Pages :

e70256

Peer reviewed :

Peer Reviewed verified by ORBi

Additional URL :

https://bsppjournals.onlinelibrary.wiley.com/doi/pdf/10.1111/mpp.70256

Funding text :

This study was supported by the Zhejiang Provincial Natural Science Foundation of China under Grant No. LD25C140001; the 2024 Science and Technology Support Project of the Inner Mongolia Innovation Center of Biological Breeding Technology (Grant No. 2023DXZD0001, 2024NSZC06); the Key Laboratory of Rice Breeding Innovation in Northern Cold Inner Mongolia (Grant No. YZ2023004); the Nanfan special project, CAAS (Grant No. YBXM2567); the Sanya Special Fund for Scientific and Technological Innovation (Grant No. 2022KJCX45); and the Heilongjiang Key Research and Development Program (Grant No. 2025ZX04B02). We thank Dr. Yuese Ning from the Institute of Plant Protection, CAAS, for providing the RFP\u2010tagged vectors. We thank Dr. Qiangzhi Li from the Institute of Plant Protection, CAAS, for providing the mitochondrial and peroxisomal markers.This work was supported by Zhejiang Provincial Natural Science Foundation of China, LD25C140001. The 2024 Science and Technology Support Project of the Inner Mongolia Innovation Center of Biological Breeding Technology, 2024NSZC06, 2023DXZD0001. The Key Laboratory of Rice Breeding Innovation in Northern Cold Inner Mongolia, YZ2023004. The Nanfan special project, CAAS, YBXM2567. The Sanya Special Fund for Scientific and Technological Innovation, 2022KJCX45. The Heilongjiang Key Research and Development Program, 2025ZX04B02. This study was supported by the Zhejiang Provincial Natural Science Foundation of China under Grant No. LD25C140001; the 2024 Science and Technology Support Project of the Inner Mongolia Innovation Center of Biological Breeding Technology (Grant No. 2023DXZD0001, 2024NSZC06); the Key Laboratory of Rice Breeding Innovation in Northern Cold Inner Mongolia (Grant No. YZ2023004); the Nanfan special project, CAAS (Grant No. YBXM2567); the Sanya Special Fund for Scientific and Technological Innovation (Grant No. 2022KJCX45); and the Heilongjiang Key Research and Development Program (Grant No. 2025ZX04B02). We thank Dr. Yuese Ning from the Institute of Plant Protection, CAAS, for providing the RFP-tagged vectors. We thank Dr. Qiangzhi Li from the Institute of Plant Protection, CAAS, for providing the mitochondrial and peroxisomal markers.This work was supported by Zhejiang Provincial Natural Science Foundation of China, LD25C140001. The 2024 Science and Technology Support Project of the Inner Mongolia Innovation Center of Biological Breeding Technology, 2024NSZC06, 2023DXZD0001. The Key Laboratory of Rice Breeding Innovation in Northern Cold Inner Mongolia, YZ2023004. The Nanfan special project, CAAS, YBXM2567. The Sanya Special Fund for Scientific and Technological Innovation, 2022KJCX45. The Heilongjiang Key Research and Development Program, 2025ZX04B02.

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