hibernation; photoperiod; seasonal; tanycyte; torpor; Ecology, Evolution, Behavior and Systematics; Animal Science and Zoology; Mesocricetus auratus; hypothalamus
Abstract :
[en] Hibernation is a physiological and behavioural adaptation that permits survival during periods of reduced food availability and extreme environmental temperatures. This is achieved through episodes of metabolic depression and body cooling (torpor), and subsequent rewarming (arousal), cycling between these states is presumed to stem from changes in hypothalamic metabolic control. Several recent lines of evidence implicate the hypothalamic tanycytes in this phenomenon. To investigate tanycytic changes over the hibernation season, golden hamsters (Mesocricetus auratus (Waterhouse, 1839)) were transferred from long photoperiod 22◦C to short photoperiod (SP) 8◦C, sampling animals at physiologically defined points across the hibernation season for LASER capture microdissection, and RNAseq of the tanycytic region. Our analysis revealed a marked reduction in the expression of genes linked to ciliary assembly and G-protein coupled receptor-signalling during the hibernation season, as well as evidence for a shift towards increased glycolytic metabolism. These aspects were all reversed in refractory animals, which spontaneously ceased to express torpor after extended exposure to SP 8◦C. Tanycytes sampled mid-torpor show increased expression of immediate-early genes compared to the interbout euthermic state, while genes linked to RNA processing and translation show the reverse effect. The implications of these findings for the putative involvement of tanycytes in hibernation control mechanisms are discussed.
Melum, Vebjørn. J.; Arctic seasonal timekeeping initiative (ASTI), UiT —The Arctic University of Norway, Department of Arctic and Marine Biology, Arctic Chronobiology and Physiology Research Group, Tromsø, Norway ; University of Strasbourg, Institute of Cellular and Integrative Neurosciences, Strasbourg, France
Bothorel, Béatrice; University of Strasbourg, Institute of Cellular and Integrative Neurosciences, Strasbourg, France
Moralia, Marie-Azélie ; Université de Liège - ULiège > Département des sciences cliniques > Pédiatrie ; University of Strasbourg, Institute of Cellular and Integrative Neurosciences, Strasbourg, France
Simonneaux, Valérie; University of Strasbourg, Institute of Cellular and Integrative Neurosciences, Strasbourg, France
Hazlerigg, David G.; Arctic seasonal timekeeping initiative (ASTI), UiT —The Arctic University of Norway, Department of Arctic and Marine Biology, Arctic Chronobiology and Physiology Research Group, Tromsø, Norway
Wood, Shona H. ; Arctic seasonal timekeeping initiative (ASTI), UiT —The Arctic University of Norway, Department of Arctic and Marine Biology, Arctic Chronobiology and Physiology Research Group, Tromsø, Norway
Language :
English
Title :
Altered gene expression related to fuel use and sensory capacity in tanycytes throughout the hibernation season in the golden hamster
TFS - Tromsø Forskningsstiftelse ERC - European Research Council
Funding text :
The work was supported by grants from the Troms\u00F8 forskningsstiftelse (TFS) starter grant TFS2016SW and the TFS infrastructure grant (IS3_17_SW) awarded to SHW. It was also co-funded by the European Union (ERC, HiTime, 101086671). Views and opinions expressed are however those of the author(s) only and do not necessarily reflect those of the European Union or the European Research Council. Neither the European Union nor the granting authority can be held responsible for them. The Arctic seasonal timekeeping initiative (ASTI) grant and UiT strategic funds support DGH and SHW.
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