Apoptosis; Biosynthesis; Camellia sinensis; Cancer therapy; NTERA-2; Apoptotic; Cancer stem cells; Cytotoxic; Embryonics; Green tea; Property; Synthesised; Bioengineering; Biochemistry; Applied Microbiology and Biotechnology
Abstract :
[en] Silver nanoparticles (AgNPs) are known as carriers of anticancer activities for effectual target treatment of cancer cells. Plant-mediated synthesis for AgNPs synthesis is valuable for biomedical applications due to their more safety and cost-effectiveness. In this work, AgNPs were synthesized successfully using green tea (Camellia sinensis) extract (CSE-AgNPs). The physicochemical properties of CSE-AgNPs were identified by UV–visible spectrophotometry, FT-IR, PXRD, DLS and TEM. The results indicated the successful synthesis of well-dispersed CSE-AgNPs with spherical shape and size (8–68 nm). The cytotoxic effects of CSE-AgNPs on hominid testicular embryonic cancer stem cells (NTERA-2) and normal cells (ACTH2 cell line) were done by the MTT assay and acridine orange/propidium iodide staining. CSE-AgNPs exhibited toxic effects in a dose-dependent manner as their half inhibitory concentrations (IC50) were 6 μg/mL and 28 μg/mL against the cancerous and normal cells after 24 h, respectively. The investigation of Bcl2, Bax, caspase 3 and 9, p53 and HSPA2 genes' expression indicated CSE-AgNPs as an apoptosis-inducing complex. The findings document the usability of the biosynthesized CSE-AgNPs for triggering cell death in cancerous cells in vitro and provide a strong rationale for conducting in vivo studies.
Disciplines :
Laboratory medicine & medical technology
Author, co-author :
Mobaraki, Fahimeh; Department of Anatomy and Cell Biology, School of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran
Momeni, Mohsen ; Université de Liège - ULiège > Département des sciences biomédicales et précliniques > Biologie cellulaire et moléculaire ; Immuno-Biochemistry lab, Immunology Research Center, Mashhad University of Medical Sciences, Mashhad, Iran
Jahromi, Maliheh; Department of Medicine, Najafabad Branch, Islamic Azad University, Najafabad, Iran
Kasmaie, Farshad Moharrami; Department of Biology and Anatomical Sciences, School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran
Barghbani, Maryam; Department of Medical Biotechnology, School of Allied Medicine, Qazvin University of Medical Sciences, Qazvin, Iran
Yazdi, Mohammad Ehsan Taghavizadeh; Applied Biomedical Research Center, School of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran
Meshkat, Zahra; Department of Microbiology and Virology, School of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran
Shandiz, Fatemeh Homaee; Cancer Research Center, Mashhad University of Medical Sciences, Mashhad, Iran
Hosseini, Seyed Mousalreza; Department of Gastroenterology, School of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran ; Surgical Oncology Research Center, Mashhad University of Medical Sciences, Mashhad, Iran
Language :
English
Title :
Apoptotic, antioxidant and cytotoxic properties of synthesized AgNPs using green tea against human testicular embryonic cancer stem cells
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