Extract-mediated biosynthesis and characterization of gold nanoparticles: Exploring their protective effect against cyclophosphamide-induced oxidative stress in rat testis - 2022
Extract-mediated biosynthesis and characterization of gold nanoparticles: Exploring their protective effect against cyclophosphamide-induced oxidative stress in rat testis
[en] The biosynthesis method is essential for minimizing toxic by-products caused by conventional nanoparticle synthesis. Adding a surface biological coating to gold nanoparticles (AuNPs) makes them durable, adaptable, and cost-effective. Herein, Nasturtium officinale leaf extract was used as a reducing and stabilizing agent. UV–vis spectrophotometer, X-ray diffraction, Fourier-transform infrared spectroscopy, transmission electron microscopy, dynamic light scattering, and Zeta potential methods were employed to assess the nanoparticles' formation, crystallinity, size, shape, and surface charge properties. Spherical AuNPs were produced with an average size of 64 nm and demonstrated dose-dependent DPPH scavenging property. To achieve the non-toxic dose of the AuNPs, the MTT assay and AO/PI were performed on the rat bone marrow cells. The co-administration of AuNPs and cyclophosphamide intraperitoneally prevented testicular damages in male rats. In cyclophosphamide-induced gonadotoxicity, the AuNPs' cytoprotective effect was depicted by its reversal of hormonal and morphological alterations toward normality. A reverse transcription-polymerase chain reaction (RT-PCR) analysis was accomplished on apoptotic genes' expression (caspase-3, caspase-9, Bax, Bcl2) to confirm that the biosynthesized nanoparticles could act as a fascinating antioxidant and exert protective effects against apoptosis. These positive results can provide a rational and potential source for a novel conjugation system of chemotherapy drugs with AuNPs in cancer treatment with fewer side effects.
Disciplines :
Laboratory medicine & medical technology
Author, co-author :
Mobaraki, Fahimeh; Department of Anatomy and Cell Biology, School of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran
Momeni, Mohsen ; Université de Liège - ULiège > Département des sciences biomédicales et précliniques > Biologie cellulaire et moléculaire ; Immuno-Biochemistry Lab, Immunology Research Center, Mashhad University of Medical Sciences, Mashhad, Iran
Barghbani, Maryam; Department of Medical Biotechnology, School of Allied Medicine, Qazvin University of Medical Sciences, Qazvin, Iran
Far, Bahareh Farasati; Department of Chemistry, Iran University of Science and Technology, Tehran, Iran
Hosseinian, Sara; Department of Medical Physiology, School of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran ; Applied Biomedical Research Center, Mashhad University of Medical Sciences, Mashhad, Iran
Hosseini, Seyed Mousalreza ; Department of Gastroenterology, School of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran ; Surgical Oncology Research Center, Mashhad University of Medical Sciences, Mashhad, Iran
Language :
English
Title :
Extract-mediated biosynthesis and characterization of gold nanoparticles: Exploring their protective effect against cyclophosphamide-induced oxidative stress in rat testis
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