Protocol for whole-cell patch-clamp recording and post hoc identification of hippocampal CA2 pyramidal neurons in adult mouse brain slices.

Engel, Dominique; Nguyen, Laurent

doi:10.1016/j.xpro.2026.104470

Article (Scientific journals)

Protocol for whole-cell patch-clamp recording and post hoc identification of hippocampal CA2 pyramidal neurons in adult mouse brain slices.

Engel, Dominique; Nguyen, Laurent

2026 • In STAR Protocols, 7 (2), p. 104470

Peer Reviewed verified by ORBi

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https://hdl.handle.net/2268/343782

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10.1016/j.xpro.2026.104470

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41903142

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Keywords :

Cell Biology; Microscopy; Model Organisms; Neuroscience

Abstract :

[en] We present a protocol for whole-cell recordings from hippocampal CA2 pyramidal neurons in acute adult mouse brain slices. We describe the preparation of viable dorsal hippocampal slices, the visual identification and recording of CA2 neurons, and intracellular labeling. We then detail post hoc immunohistochemical verification using CA2-specific markers. This approach enables reliable functional characterization of CA2 neurons and unambiguous confirmation of their anatomical localization. For complete details on the use and execution of this protocol, please refer to Le Bail et al.1.

Research Center/Unit :

GIGA Neurosciences-Molecular Regulation of Neurogenesis - ULiège

Disciplines :

Biochemistry, biophysics & molecular biology

Author, co-author :

Engel, Dominique ; Université de Liège - ULiège > Département des sciences biomédicales et précliniques > Pharmacologie

Nguyen, Laurent ; Université de Liège - ULiège > Département des sciences biomédicales et précliniques

Language :

English

Title :

Protocol for whole-cell patch-clamp recording and post hoc identification of hippocampal CA2 pyramidal neurons in adult mouse brain slices.

Original title :

[en] Protocol for whole-cell patch-clamp recording and post hoc identification of hippocampal CA2 pyramidal neurons in adult mouse brain slices.

Publication date :

26 March 2026

Journal title :

STAR Protocols

eISSN :

2666-1667

Publisher :

Elsevier BV, United States

Volume :

Issue :

Pages :

104470

Peer reviewed :

Peer Reviewed verified by ORBi

Additional URL :

https://api.elsevier.com/content/article/PII:S2666166726001231?httpAccept=text/xml

Funders :

F.R.S.-FNRS - Fund for Scientific Research

Available on ORBi :

since 08 April 2026

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