development; membrane properties; motor cortex; motor neurons; patch clamp; pyramidal neurons; Molecular Biology; Cellular and Molecular Neuroscience
Abstract :
[en] Achieving the distinctive complex behaviors of adult mammals requires the development of a great variety of specialized neural circuits. Although the development of these circuits begins during the embryonic stage, they remain immature at birth, requiring a postnatal maturation process to achieve these complex tasks. Understanding how the neuronal membrane properties and circuits change during development is the first step to understand their transition into efficient ones. Thus, using whole cell patch clamp recordings, we have studied the changes in the electrophysiological properties of layer V pyramidal neurons of the rat primary motor cortex during postnatal development. Among all the parameters studied, only the voltage threshold was established at birth and, although some of the changes occurred mainly during the second postnatal week, other properties such as membrane potential, capacitance, duration of the post-hyperpolarization phase or the maximum firing rate were not defined until the beginning of adulthood. Those modifications lead to a decrease in neuronal excitability and to an increase in the working range in young adult neurons, allowing more sensitive and accurate responses. This maturation process, that involves an increase in neuronal size and changes in ionic conductances, seems to be influenced by the neuronal type and by the task that neurons perform as inferred from the comparison with other pyramidal and motor neuron populations.
Perez-García, Patricia; Department of Physiology, School of Pharmacy, University of Seville, Seville, Spain ; Division of Physiology, School of Medicine, University of Cádiz, Cádiz, Spain
Pardillo-Díaz, Ricardo; Division of Physiology, School of Medicine, University of Cádiz, Cádiz, Spain ; Biomedical Research and Innovation Institute of Cádiz (INiBICA), Cádiz, Spain
Geribaldi-Doldán, Noelia; Biomedical Research and Innovation Institute of Cádiz (INiBICA), Cádiz, Spain ; Department of Human Anatomy and Embriology, University of Cádiz, Cádiz, Spain
Gómez Oliva, Ricardo ; Université de Liège - ULiège > GIGA > GIGA Neurosciences - Molecular Regulation of Neurogenesis ; Division of Physiology, School of Medicine, University of Cádiz, Cádiz, Spain ; Biomedical Research and Innovation Institute of Cádiz (INiBICA), Cádiz, Spain
Domínguez-García, Samuel; Division of Physiology, School of Medicine, University of Cádiz, Cádiz, Spain ; Biomedical Research and Innovation Institute of Cádiz (INiBICA), Cádiz, Spain
Castro, Carmen; Division of Physiology, School of Medicine, University of Cádiz, Cádiz, Spain ; Biomedical Research and Innovation Institute of Cádiz (INiBICA), Cádiz, Spain
Nunez-Abades, Pedro; Department of Physiology, School of Pharmacy, University of Seville, Seville, Spain ; Biomedical Research and Innovation Institute of Cádiz (INiBICA), Cádiz, Spain
Carrascal, Livia; Department of Physiology, School of Pharmacy, University of Seville, Seville, Spain ; Biomedical Research and Innovation Institute of Cádiz (INiBICA), Cádiz, Spain
Language :
English
Title :
Refinement of Active and Passive Membrane Properties of Layer V Pyramidal Neurons in Rat Primary Motor Cortex During Postnatal Development.
MICINN - Ministerio de Ciencia, Innovación y Universidades ERDF - European Regional Development Fund
Funding text :
This work was partially supported by Spanish Ministerio de Ciencia, Innovación y Universidades (RTI2018-099908-BC21) and co-financed by the 2014–2020 ERDF Operational Programme and by the Department of Economy, Knowledge, Business and University of the Regional Government of Andalusia (FEDER-UCA18-106647).We sincerely thank Ella Gorton, a visiting fellow of the University of Manchester, United Kingdom, supported by an Erasmus Practices grant, for her initial contribution to this work.
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