amyotrophic lateral sclerosis; glutathione levels in the brain; membrane excitability; motor cortex; oxidative stress; postnatal development; Food Science; Physiology; Biochemistry; Molecular Biology; Clinical Biochemistry; Cell Biology
Abstract :
[en] Oxidative stress is one of the main proposed mechanisms involved in neuronal degeneration. To evaluate the consequences of oxidative stress on motor cortex pyramidal neurons during postnatal development, rats were classified into three groups: Newborn (P2-P7); infantile (P11-P15); and young adult (P20-P40). Oxidative stress was induced by 10 µM of cumene hydroperoxide (CH) application. In newborn rats, using the whole cell patch-clamp technique in brain slices, no significant modifications in membrane excitability were found. In infantile rats, the input resistance increased and rheobase decreased due to the blockage of GABAergic tonic conductance. Lipid peroxidation induced by CH resulted in a noticeable increase in protein-bound 4-hidroxynonenal in homogenates in only infantile and young adult rat slices. Interestingly, homogenates of newborn rat brain slices showed the highest capacity to respond to oxidative stress by dramatically increasing their glutathione and free thiol content. This increase correlated with a time-dependent increase in the glutathione reductase activity, suggesting a greater buffering capacity of newborn rats to resist oxidative stress. Furthermore, pre-treatment of the slices with glutathione monoethyl ester acted as a neuroprotector in pyramidal neurons of infantile rats. We conclude that during maturation, the vulnerability to oxidative stress in rat motor neurons increases with age.
Carrascal, Livia; Departament of Physiology, Pharmacy School, University of Seville, 41012 Seville, Spain ; Biomedical Research and Innovation Institute of Cadiz (INIBICA), 11003 Cadiz, Spain
Gorton, Ella; Departament of Physiology, Pharmacy School, University of Seville, 41012 Seville, Spain
Pardillo-Díaz, Ricardo; Area of Physiology, School of Medicine, University of Cádiz, 11003 Cadiz, Spain ; Biomedical Research and Innovation Institute of Cadiz (INIBICA), 11003 Cadiz, Spain
Perez-García, Patricia; Departament of Physiology, Pharmacy School, University of Seville, 41012 Seville, Spain
Gómez Oliva, Ricardo ; Université de Liège - ULiège > GIGA > GIGA Neurosciences - Molecular Regulation of Neurogenesis ; Area of Physiology, School of Medicine, University of Cádiz, 11003 Cadiz, Spain ; Biomedical Research and Innovation Institute of Cadiz (INIBICA), 11003 Cadiz, Spain
Castro, Carmen ; Area of Physiology, School of Medicine, University of Cádiz, 11003 Cadiz, Spain ; Biomedical Research and Innovation Institute of Cadiz (INIBICA), 11003 Cadiz, Spain
Nunez-Abades, Pedro ; Departament of Physiology, Pharmacy School, University of Seville, 41012 Seville, Spain ; Biomedical Research and Innovation Institute of Cadiz (INIBICA), 11003 Cadiz, Spain
Language :
English
Title :
Age-Dependent Vulnerability to Oxidative Stress of Postnatal Rat Pyramidal Motor Cortex Neurons.
MICINN - Ministerio de Ciencia, Innovación y Universidades Junta de Andalucía. Consejería de Economía, Conocimiento, Empresas y Universidad
Funding text :
Funding: This work was partially supported by Spanish Ministerio de Ciencia, Innovación y Universidades (RTI2018-099908-B-C21) and co-financed by the 2014-2020 ERDF Operational Programme and by the Department of Economy, Knowledge, Business and University of the Regional Government of Andalusia (FEDER-UCA18-106647) Acknowledgments: Ella Gorton is a visiting fellow of the University of Manchester, UK, supported by a Erasmus Practicas grant. Ricardo Gomez-Oliva is a fellow of the University of Cadiz fellowship program. Ricardo Pardillo-Díaz is a postdoctoral fellow of the University of Cadiz.This work was partially supported by Spanish Ministerio de Ciencia, Innovaci?n y Universidades (RTI2018-099908-B-C21) and co-financed by the 2014-2020 ERDF Operational Programme and by the Department of Economy, Knowledge, Business and University of the Regional Government of Andalusia (FEDER-UCA18-106647).
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