Performance; breast muscle; fatty acids; oil; Food Science; Animal Science and Zoology
Abstract :
[en] 1. The objective of this trial was to examine the effect of Sacha inchi oil (SIO), extracted from local nut sources common to the Amazon region, in broiler diets. The oil was substituted for soybean oil (SBO) within diets of slow-growing broilers and the effects on growth performance, carcass traits, serum biochemistry, objective meat quality and meat fatty acid profile were assessed.2. A total of 400, mixed sex, 56 d old, crossbred Ho × Luong Phuong broilers were randomly allocated to one of four experimental diets: a control diet (CON) containing 4.5% SBO and three experimental diets with SIO at substitution levels of 33.3% (SI1), 66.7% (SI2) and 100% (SI3) instead of SBO for a feeding period of 56 days. Each diet was replicated in four replicated pens of 25 birds, comprising two pens of males and two pens of females.3. Growth variables were recorded every two weeks. Blood samples were collected via puncture of the brachial vein one day prior to the completion of the experiment to determine serum biochemical indices. At the end of the study, four birds per pen were humanely slaughtered to evaluate carcass traits and breast samples were collected for assessments of meat technological quality and fatty acid profile.4. The inclusion of SIO increased the proportion of n-3 PUFA, lowered the n-6/n-3 PUFA ratio and cholesterol content in breast muscle and reduced serum triglyceride and low-density lipoprotein levels, without detrimental effects on growth, carcass traits or meat quality variables.5. The results demonstrated that SIO is a promising alternative lipid source for improving the nutritional value of poultry meat without adversely affecting productive performance or carcass characteristics.
Disciplines :
Animal production & animal husbandry
Author, co-author :
Nguyen Cong, O ; Faculty of Animal Science, Vietnam National University of Agriculture, Hanoi, Vietnam
Hornick, Jean-Luc ; Université de Liège - ULiège > Département de gestion vétérinaire des Ressources Animales (DRA)
Nguyen, D V ; Faculty of Animal Science, Vietnam National University of Agriculture, Hanoi, Vietnam ; School of Environmental and Rural Science, University of New England, Armidale, Australia
Language :
English
Title :
Sacha inchi (Plukenetia volubilis L.) oil inclusion in slow-growing broiler diets: impacts on performance, serum biochemistry, carcass, meat quality and fatty acid profile.
Alagawany, M., S. S., Elnesr, M. R., Farag, M. E., abd el-hack, A. F., Khafaga, A. E., Taha, R., Tiwari, et al. 2019. “Omega-3 and Omega-6 Fatty Acids in Poultry Nutrition: Effect on Production Performance and Health.” Animals9 (8): 573. https://doi.org/10.3390/ani9080573.
Alagawany, M., S. S., Elnesr, M. R., Farag, K., el-sabrout, O., Alqaisi, M. A., Awood, H., Soomro, and S. A., Abdelnour. 2022. “Nutritional Significance and Health Benefits of Omega-3, -6 and -9 Fatty Acids in Animals.” Animal Biotechnology33 (7): 1678–1690. https://doi.org/10.1080/10495398.2020.1869562.
Aoac. 1990. Official Methods of Analysis. 15th ed. Association of Official Analytical Chemists.
Aprianto, M. A., A., Kurniawati, C., Hanim, B., Ariyadi, and M., Al Anas. 2023. “Effect Supplementation of Black Soldier Fly Larvae Oil (Hermetia Illucens L.) Calcium Salt on Performance, Blood Biochemical Profile, Carcass Characteristic, Meat Quality and Gene Expression in Fat Metabolism Broilers.” Poultry Science102 (10): 102984. https://doi.org/10.1016/j.psj.2023.102984.
Attia, Y. A., M. A., al-harthi, and H. M., Abo el-maaty. 2020. “The Effects of Different Oil Sources on Performance, Digestive Enzymes, Carcass Traits, Biochemical, Immunological, Antioxidant and Morphometric Responses of Broiler Chicks.” Frontiers in Veterinary Science28 (7): 181. https://doi.org/10.3389/fvets.2020.00181.
Bai, H., B., Yang, Z., Dong, X., Li, Q., Song, Y., Jiang, G., Chang, and G., Chen. 2022. “Research Note: Effects of Cage and Floor Rearing Systems on Growth Performance, Carcass Traits, and Meat Quality in Small-Sized Meat Ducks.” Poultry Science101 (1): 101520. https://doi.org/10.1016/j.psj.2021.101520.
Beauclercq, S., S., Mignon-grasteau, A., Petit, Q., Berger, A., Lefèvre, S., Metayer-coustard, S., Tesseraud, P., Emond, C., Berri, and E. L., Bihan-duval. 2022. “A Divergent Selection on Breast Meat Ultimate pH, a Key Factor for Chicken Meat Quality, Is Associated with Different Circulating Lipid Profiles.” Frontiers in Physiology13:935868. https://doi.org/10.3389/fphys.2022.935868.
Birhanu, M. Y., K., Geremew, W., Esatu, W., Worku, S., Getachew, F., Unger, and T., Dessie. 2021. “Poultry Production, Marketing and Consumption in Vietnam: A Review of Literature.” ILRI Research Report. access on 1April 2025. http://cgspace.cgiar.org/server/api/core/bitstreams/6242b3b0-4263-4601-8098-33e45221d41d/content.
Bligh, E. G., and W. J., Dyer. 1959. “A Rapid Method of Total Lipid Extraction and Purification.” Canadian Journal of Biochemistry and Physiology37 (8): 911–917. https://doi.org/10.1139/o59-099.
Cárdenas, D. M., L. J., gómez Rave, and J. A., Soto. 2021. “Biological Activity of Sacha Inchi (Plukenetia Volubilis Linneo) and Potential Uses in Human Health: A Review.” Food Technology and Biotechnology59 (3): 253–266. https://doi.org/10.17113/ftb.59.03.21.6683.
Choi, J., B., Kong, B. C., Bowker, H., Zhuang, and W. K., Kim. 2023. “Nutritional Strategies to Improve Meat Quality and Composition in the Challenging Conditions of Broiler Production: A Review.” Animals13 (8): 1386. https://doi.org/10.3390/ani13081386.
Cobb-Vantress. 2018. “Broiler Performance & Nutrition Supplement. L-2114-08 EN.” Arkansas, US, Cobb Vantress Inc. access on 1April 2024. https://appec-h.com/wp-content/uploads/2023/02/Broiler-Performance-and-Nutrition-Supplement.pdf.
Dai, Q. V., M., Yabe, H., Nomura, and Y., Takahashi. 2022. “Structural Changes in Meat Consumption in Vietnam: Evidence from Household Survey Data.” Journal of Agribusiness in Developing and Emerging Economies13 (4): 590–612. https://doi.org/10.1108/JADEE-08-2021-0206.
d’Angelo, S., M. L., Motti, and R., Meccariello. 2020. “ω-3 and ω-6 Polyunsaturated Fatty Acids, Obesity and Cancer.” Nutrients12 (9): 2751. https://doi.org/10.3390/nu12092751.
Derewiaka, D., and M., Obiedziński. 2010. “Cholesterol Oxides Content in Selected Animal Products Determined by GC-MS.” European Journal of Lipid Science and Technology112 (10): 1130–1137. https://doi.org/10.1002/ejlt.200900238.
Doan, B. H., N. T., Mai, N. T., Son, and N. H., Dat. 2011. Studied Parameters in the Poultry Production in Vietnam. Hanoi, Vietnam: Agricultural Publishing House.
European Union. 2009. “Commission Regulation (EC) No 152/2009 of 27 January 2009 Laying Down the Methods of Sampling and Analysis for the Official Control of Feed.” Official Journal of the European Union54 (1): 2–54. access on 1August 2024. http://data.europa.eu/eli/reg/2009/152/oj.
Geng, A. L., Q. Q., Zhang, C., Chang, H. H., Wang, Q., Chu, J., Zhang, Z. X., Yan, and H. G., Liu. 2023. “Dietary Metabolizable Energy and Crude Protein Levels Affect the Performance, Egg Quality and Biochemical Parameters of a Dual-Purpose Chicken.” Animal Biotechnology34 (7): 2714–2723. https://doi.org/10.1080/10495398.2022.2114001.
González Sepúlveda, C. A., Á. M., Giraldo Mejía, and E. A., Oviedo Álvarez. 2024. “Evaluation of the Inclusion of Three Oils on Productive Parameters and Carcass Characteristics in Broiler.” Revista Facultad Nacional de Agronomía Medellín77 (1): 10637–10649. https://doi.org/10.15446/rfnam.v77n1.107938.
Goyal, A., B., Tanwar, M. K., Sihag, and V., Sharma. 2022. “Sacha Inchi (Plukenetia Volubilis L.): An Emerging Source of Nutrients, Omega-3 Fatty Acid and Phytochemicals.” Food Chemistry373 (March): 131459. https://doi.org/10.1016/j.foodchem.2021.131459.
Ivanova, P., P. B., Petrov, V., Gerzilov, N., Keranova, Z., Petkova, O., Teneva, G., Antova, P., Hristakieva, and I. G., Penchev. 2022. “Effect of Flaxseed Oil Supplemented Diets on Growth Performance and Meat Quality Traits in Broilers.” Bulgarian Journal of Agricultural Science28 (1): 101–110.
Kanakri, K., J., Carragher, R., Hughes, B., Muhlhausler, and R., Gibson. 2017. “A Reduced Cost Strategy for Enriching Chicken Meat with Omega-3 Long Chain Polyunsaturated Fatty Acids Using Dietary Flaxseed Oil.” British Poultry Science58 (3): 283–289. https://doi.org/10.1080/00071668.2017.1293798.
Long, S., S., Liu, D., Wu, S., Mahfuz, and X., Piao. 2020. “Effects of Dietary Fatty Acids from Different Sources on Growth Performance, Meat Quality, Muscle Fatty Acid Deposition, and Antioxidant Capacity in Broilers.” Animals10 (3): 508. https://doi.org/10.3390/ani10030508.
Lopes, D. C. N., E. G., Xavier, V. L., Santos, F. M., Goncalves, M. A., Anciuti, V. F. B., Roll, F. A. B. D., Pino, J. O., Feijo, and A. A. S., Catalan. 2013. “Growth Performance, Carcass Traits, Meat Chemical Composition and Blood Serum Metabolites of Broiler Chicken Fed on Diets Containing Flaxseed Oil.” British Poultry Science54 (6): 780–788. https://doi.org/10.1080/00071668.2013.843161.
Lu, W. C., C. S., Chiu, Y. J., Chan, A. T., Mulio, and P. H., Li. 2025. “New Perspectives on Different Sacha Inchi Seed Oil Extractions and Its Applications in the Food and Cosmetic Industries.” Critical Reviews in Food Science and Nutrition65 (3): 475–493. https://doi.org/10.1080/10408398.2023.2276882.
Lucas, J., E., Icochea, R., Valdivia, F., Carcelén, and J., Guzmán. 2011. “Efecto del Aceite de Sacha Inchi (Plukenetia Volubilis) en la Dieta de Reproductoras de Pollos de Engorde sobre el Desempeño Productivo de su Progenie.” Revista de Investigaciones Veterinarias del Perú22 (4): 283–289. https://doi.org/10.15381/rivep.v22i4.327.
Mai, H. C., N. C., Duy, N. P. T., Nhan, and B. G., Long. 2020. “Physico-Chemical Properties of Sacha Inchi (Plukenetia Volubilis L.) Seed Oil from Vietnam.” Asian Journal of Chemistry32 (2): 2. https://doi.org/10.14233/ajchem.2020.22233.
MARD. 2019. Decision of the Ministry of Agriculture and Rural Development (MARD) on Special Recognition of New Medicinal Plant. 204/QD-BNN-TT.Hanoi, Vietnam: Ministry of Agriculture and Rural Development.
Mhlongo, G., and C. M., Mnisi. 2023. “Effect of Seaweed (Ecklonia maxima) on Apparent Nutrient Digestibility, Growth Performance and Physiological and Meat Quality Parameters in Boschveld Cockerels.” Poultry Science102 (2): 102361. https://doi.org/10.1016/j.psj.2022.102361.
Mirshekar, R., F., Boldaji, B., Dastar, A., Yamchi, and S., Pashaei. 2015. “Longer Consumption of Flaxseed Oil Enhances N-3 Fatty Acid Content of Chicken Meat and Expression of FADS2 Gene.” European Journal of Lipid Science and Technology117 (6): 810–819. https://doi.org/10.1002/ejlt.201300500.
Mirshekar, R., B., Dastar, and M. S., Shargh. 2021. “Supplementing Flaxseed Oil for Long Periods Improved Carcass Quality and Breast Fatty Acid Profile in Japanese Quail.” Animal15 (2): 100104. https://doi.org/10.1016/j.animal.2020.100104.
Nájera, P. R. A., H. I. V., Lara, S. E. L., Sampedro, and H., Patricio. 2018. “Evaluation of Three Levels of Sacha Inchi Oil (Plukenetia volubilis) in Broilers Chicken Diet.” Revista Caribeña de Ciencias Sociales 5: 1–24. http://www.eumed.net/rev/caribe/2018/05/dieta-pollos-engorde.html.
Nguyen, D. V., A. R., Flakemore, J. R., Otto, S. W., Ives, R. W., Smith, P. D., Nichols, and A. E. O., Malau-aduli. 2017a. “Nutritional Value and Sensory Characteristics of Meat Eating Quality of Australian Prime Lambs Supplemented with Pelleted Canola and Flaxseed Oils: Fatty Acid Profiles of Muscle and Adipose Tissues.” Internal Medicine Review3 (3): 1–21.
Nguyen, D. V., B. S., Malau-aduli, J., Cavalieri, P. D., Nichols, and A. E., Malau-aduli. 2018. “Supplementation With Plant-Derived Oils Rich in Omega-3 Polyunsaturated Fatty Acids for Lamb Production.” Veterinary and Animal Science6:29–40. https://doi.org/10.1016/j.vas.2018.08.001.
Nguyen, D. V., H. L., van, N. V., Quang, B. S., Malau-aduli, P. D., Nichols, and A. E., Malau-aduli. 2017b. “Omega–3 Long-Chain Fatty Acids in the Heart, Kidney, Liver and Plasma Metabolite Profiles of Australian Prime Lambs Supplemented with Pelleted Canola and Flaxseed Oils.” Nutrients9 (8): 893. https://doi.org/10.3390/nu9080893.
Oanh, N. C., N. V., Don, P. K., Dang, and J.-L., Hornick. 2022. “Effects of Dietary Sacha Inchi (Plukenetia volubilis L.) Oil and Medicinal Plant Powder Supplementation on Growth Performance, Carcass Traits and Breast Meat Quality of Colored Broiler Chickens Raised in Vietnam.” Tropical Animal Health and Production54 (2): 1–9. https://doi.org/10.1007/s11250-021-02994-8.
Park, S. Y., D. S., Byeon, G. W., Kim, and H. Y., Kim. 2021. “Carcass and Retail Meat Cuts Quality Properties of Broiler Chicken Meat Based on the Slaughter Age.” Journal of Animal Science and Technology63 (1): 180. https://doi.org/10.5187/jast.2021.e2.
Phuong, N. V., T. H., Cuong, and M., Mergenthaler. 2014. “Effects of Socio-Economic and Demographic Variables on Meat Consumption in Vietnam.” Asian Journal of Agriculture and Rural Development4 (393–2016–23934): 7–22. access on 1April 2024. https://archive.aessweb.com/index.php/5005/article/view/1115.
Qassim, A. H. A., L. K., Ban, and F. M., Alkalan. 2022. “Effect of Using Avocado and Chia Oil and Their Mixture in Meat Broiler Diets on Some Physiological and Microbial Characteristics of Lood Plasma.” NeuroQuantology20 (4): 233–243. https://doi.org/10.14704/nq.2022.20.4.NQ22114.
Rahbari, S., A., Salehi, S. D., Sharifi, and S., Honarbakhsh. 2025. “Dietary Omega-3 Fatty Acids Affect the Growth Performance of Broiler Chickens Reared at High Stocking Density.” Poultry Science104 (1): 104468. https://doi.org/10.1016/j.psj.2024.104468.
Schumacher, M., H., Delcurto-wyffels, J., Thomson, and J., Boles. 2022. “Fat Deposition and Fat Effects on Meat Quality - A Review.” Animals12 (12): 12. https://doi.org/10.3390/ani12121550.
Selim, S., E., Hussein, N. S., Abdel-megeid, S. J., Melebary, M. S., al-harbi, and A. A., Aleh. 2021. “Growth Performance, Antioxidant Activity, Immune Status, Meat Quality, Liver Fat Content and Liver Histomorphology of Broiler Chickens Fed Rice Bran Oil.” Animals11 (12): 3410. https://doi.org/10.3390/ani11123410.
Semjon, B., D., Marcinčáková, B., Koréneková, M., Bartkovský, J., Nagy, P., Turek, and S., Marcinčák. 2020. “Multiple Factorial Analysis of Physicochemical and Organoleptic Properties of Breast and Thigh Meat of Broilers Fed a Diet Supplemented with Humic Substances.” Poultry Science99 (3): 1750–1760. https://doi.org/10.1016/j.psj.2019.11.012.
Shoaib, M., S. A., Bhatti, S., Ashraf, M. M. A., Hamid, M. M., Javed, S., Amir, N., Aslam, et al. 2023. “Fat Digestion and Metabolism: Effect of Different Fat Sources and Fat Mobilisers in Broilers’ Diet on Growth Performance and Physiological Parameters–A Review.” Annals of Animal Science23 (3): 641–661. https://doi.org/10.2478/aoas-2022-0083.
Silalahi, M., 2022. “Sacha Inchi (Plukenetia Volubilis L.): Its Potential as Foodstuff and Traditional Medicine.” GSC Biological and Pharmaceutical Sciences18 (3): 213–218. https://doi.org/10.30574/gscbps.2022.18.3.0117.
Srikha, T., P., Pootthachaya, W., Puangsap, N., Pintaphrom, N., Somparn, W., Boonkum, A., Cherdthong, B., Tengjaroenkul, and S., Wongtangtintharn. 2024. “Effects of Black Soldier Fly Larvae Oil on Growth Performance, Blood Biochemical Parameters, Carcass Quality and Metabolomics Profile of Breast Muscle of Thai Native Chickens.” Animals14 (21): 3098. https://doi.org/10.3390/ani14213098.
Tien, V. D., V. H., Thai, N. T., Tram, and Y., Larondelle. 2019. “Evaluation and Characterization of Nutrient Value of Sacha Inchi Seeds Grown in Vietnam and the Residual Pressed Cake.” The 12th Regional Conference on Chemical Engineering, Ho Chi Minh City, Vietnam, 139–141.
Torres Sánchez, E. G., B., Hernández-ledesma, and L. F., Gutiérrez. 2023. “Sacha Inchi Oil Press-Cake: Physicochemical Characteristics, Food-Related Applications and Biological Activity.” Food Reviews International39 (1): 148–159. https://doi.org/10.1080/87559129.2021.1900231.
Verge-mèrida, G., D., Solà-oriol, A., Tres, M., Verdú, G., Farré, C., Garcés-narro, and A. C., Barroeta. 2022. “Olive Pomace Oil and Acid Oil as Alternative Fat Sources in Growing-Finishing Broiler Chicken Diets.” Poultry Science101 (10): 102079. https://doi.org/10.1016/j.psj.2022.102079.
Wang, Y., G., Wu, Y., Wang, A., Rehman, L., Yu, H., Zhang, Q., Jin, H. A. R., Suleria, and X., Wang. 2025. “Recent Developments, Challenges and Prospects of Dietary Omega-3 PUFA-Fortified Foods: Focusing on Their Effects on Cardiovascular Diseases.” Food Chemistry470:142498. https://doi.org/10.1016/j.foodchem.2024.142498.
Xie, Q., K., Xie, J., Yi, Z., Song, H., Zhang, and X., He. 2022. “The Effects of Magnolol Supplementation on Growth Performance, Meat Quality, Oxidative Capacity and Intestinal Microbiota in Broilers.” Poultry Science101 (4): 101722. https://doi.org/10.1016/j.psj.2022.101722.
Zamora, S. J., M. A., Arista, P. A., Fernández, L., Valle, H., Frias, E., Salvador-tasayco, Y. D., Leiva, et al. 2025. “Effect of Sacha Inchi Oil (Plukenetia Volubilis) on Productive Performance, Egg Quality and Blood Biochemistry of Laying Hens.” Journal of Applied Poultry Research34 (2): 100537. https://doi.org/10.1016/j.japr.2025.100537.