Xylazine; Calcium; Animals; Mice; Anesthesia; Hippocampus/physiology; Sleep; Hippocampus; Statistics and Probability; Information Systems; Education; Computer Science Applications; Statistics, Probability and Uncertainty; Library and Information Sciences
Abstract :
[en] The acute effects of anesthesia and their underlying mechanisms are still not fully understood. Thus, comprehensive analysis and efficient generalization require their description in various brain regions. Here we describe a large-scale, annotated collection of 2-photon calcium imaging data and multi-electrode, extracellular electrophysiological recordings in CA1 of the murine hippocampus under three distinct anesthetics (Isoflurane, Ketamine/Xylazine and Medetomidine/Midazolam/Fentanyl), during natural sleep, and wakefulness. We cover several aspects of data quality standardization and provide a set of tools for autonomous validation, along with analysis workflows for reuse and data exploration. The datasets described here capture various aspects of neural activity in hundreds of pyramidal cells at single cell resolution. In addition to relevance for basic biological research, the dataset may find utility in computational neuroscience as a benchmark for models of anesthesia and sleep.
Disciplines :
Life sciences: Multidisciplinary, general & others
Author, co-author :
Formozov, Andrey ; Research Group Synaptic Wiring and Information Processing, Center for Molecular Neurobiology Hamburg, University Medical Center Hamburg-Eppendorf, 20251, Hamburg, Germany. formozoff@gmail.com
Chini, Mattia ; Institute of Developmental Neurophysiology, Center for Molecular Neurobiology Hamburg, University Medical Center Hamburg-Eppendorf, 20251, Hamburg, Germany
Dieter, Alexander ; Research Group Synaptic Wiring and Information Processing, Center for Molecular Neurobiology Hamburg, University Medical Center Hamburg-Eppendorf, 20251, Hamburg, Germany
Yang, Wei; Research Group Synaptic Wiring and Information Processing, Center for Molecular Neurobiology Hamburg, University Medical Center Hamburg-Eppendorf, 20251, Hamburg, Germany
Pöpplau, Jastyn A ; Institute of Developmental Neurophysiology, Center for Molecular Neurobiology Hamburg, University Medical Center Hamburg-Eppendorf, 20251, Hamburg, Germany
Hanganu-Opatz, Ileana L; Institute of Developmental Neurophysiology, Center for Molecular Neurobiology Hamburg, University Medical Center Hamburg-Eppendorf, 20251, Hamburg, Germany
Wiegert, J Simon ; Research Group Synaptic Wiring and Information Processing, Center for Molecular Neurobiology Hamburg, University Medical Center Hamburg-Eppendorf, 20251, Hamburg, Germany. simon.wiegert@zmnh.uni-hamburg.de
Language :
English
Title :
Calcium Imaging and Electrophysiology of hippocampal Activity under Anesthesia and natural Sleep in Mice.
This work was funded by the Deutsche Forschungsgemeinschaft (DFG, German Research Foundation) - 178316478 - B8 to J.S.W. and B5 to I.L.H.-O., the European Research Council (ERC-2016-StG-714762 to J.S.W., ERC-2015-CoG-681577 to I.L.H.-O.), the Alexander von Humboldt Foundation (AvH Research Fellowship to A.F.) EU-project euSNN (MSCA-ITN-H2020\u2013860563 to I.L.H.-O.), Landesforschungsf\u00F6rderung Hamburg (LFF76, LFF73 to I.L.H.-O.), and the Chinese Scholarship Council (CSC 201606210129 to W.Y.). We thank Cynthia Rais for insightful discussions and suggestions.
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