[en] Cytokinins (CKs) represent an important class of phytohormones particularly known for their antisenescence properties that might be regulated through an effect on the oxidative metabolism. In the present work, we demonstrate the effect of CKs on catalase activity in tobacco cultivated in vitro. The catalase activity observed in suspension-cultured cells decreased slightly during the first hour of CK treatment and increased thereafter to double the level detected in untreated cells. In contrast to these results, catalase activity was inhibited in shoot cultures in which the endogenous levels of CK were elevated by the introduction of the isopentenyltransferase gene or by an exogenous feeding of CK to the cultures. Interestingly, this catalase inhibition correlated with an accumulation of scopolin, an inducible coumarin. Taken together, our results show that CK modulates (directly or undirectly) catalase activity. The inverse relationship that was always found between scopolin accumulation and catalase activity is discussed in terms of vitrification and habituation.
Petit-Paly, Geneviève; université de Tours > Biologie Moléculaire et Biochimie des Végétaux
Franck, Thierry ; Université de Liège - ULiège > Département clinique des animaux de compagnie et des équidés > Anesthésiologie gén. et pathologie chirurg. des grds animaux
Brisson, Louise; Université Laval > Biochimie > Recherche en Sciences de la Vie et de la Santé
Kevers, Claire ; Université de Liège - ULiège > Département des sciences de la vie > Biologie moléculaire et biotechnologie végétales
Chenieux, Jean Claude; Université de Tours > Biologie Moléculaire et Biochimie des Végétaux
Rideau, Marc; Université de Tours > Biologie Moléculaire et Biochimie des Végétaux
Language :
English
Title :
Cytokinin modulates catalase activity and coumarin accumulation in in vitro cultures of tobacco
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