Reference : Proteomics in Myzus persicae: effect of aphid host plant switch.
Scientific journals : Article
Life sciences : Biochemistry, biophysics & molecular biology
Proteomics in Myzus persicae: effect of aphid host plant switch.
Francis, Frédéric mailto [Université de Liège - ULiège > Gembloux Agro-Bio Tech > Gembloux Agro-Bio Tech >]
Gerkens, Pascal [> > > >]
Harmel, Nicolas [Université de Liège - ULiège > Gembloux Agro-Bio Tech > Gembloux Agro-Bio Tech >]
Mazzucchelli, Gabriel mailto [Université de Liège - ULiège > > Centre interfac. d'analyse des résidus en traces (CART) >]
De Pauw, Edwin mailto [Université de Liège - ULiège > Département de chimie (sciences) > GIGA-R : Laboratoire de spectrométrie de masse (L.S.M.) >]
Haubruge, Eric mailto [Université de Liège - ULiège > Services administratifs généraux > Vice-Recteur pour le site de Gembloux >]
Insect Biochemistry and Molecular Biology
Pergamon Press - An Imprint of Elsevier Science
Yes (verified by ORBi)
United Kingdom
[en] Adaptation, Physiological/physiology ; Animals ; Aphids/physiology ; Brassicaceae/parasitology ; Gene Expression Regulation/physiology ; Insect Proteins/biosynthesis ; Proteomics/methods ; Solanaceae/parasitology
[en] Chemical ecology is the study of how particular chemicals are involved in interactions of organisms with each other and with their surroundings. In order to reduce insect attack, plants have evolved a variety of defence mechanisms, both constitutive and inducible, while insects have evolved strategies to overcome these plant defences (such as detoxification enzymes). A major determinant of the influence of evolutionary arms races is the strategy of the insect: generalist insect herbivores, such as Myzus persicae aphid, need more complex adaptive mechanisms since they need to respond to a large array of different plant defensive chemicals. Here we studied the chemical ecology of M. persicae associated with different plant species, from Brassicaceae and Solanaceae families. To identify the involved adaptation systems to cope with the plant secondary substances and to assess the differential expression of these systems, a proteomic approach was developed. A non-restrictive approach was developed to identify all the potential adaptation systems toward the secondary metabolites from host plants. The complex protein mixtures were separated by two-dimensional electrophoresis methods and the related spots of proteins significantly varying were selected and identified by mass spectrometry (ESI MS/MS) coupled with data bank investigations. Fourteen aphid proteins were found to vary according to host plant switch; ten of them were down regulated (proteins involved in glycolysis, TCA cycle, protein and lipid synthesis) while four others were overexpressed (mainly related to the cytoskeleton). These techniques are very reliable to describe the proteome from organisms such as insects in response to particular environmental change such as host plant species of herbivores.

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