Acquisition; Aphids; PVYNTN; Retention; Serological detection; Ecology, Evolution, Behavior and Systematics; Ecology; Agronomy and Crop Science; Insect Science
Abstract :
[en] The study was carried out to investigate the ability of three aphids, Myzus persicae, Aphis gossypii and Aphis spiraecola, to acquire and retain the Potato Virus Y (PVY) isolate, PVYNTN. Tobacco plants, Nicotiana tabacum var. Xanthi, were used as test plant for the virus inoculation and aphid acquisition. The serological test double-antibody sandwich enzyme-linked immunosorbent assay was applied for virus detection on the test plants and aphids. Furthermore, virus retention by aphids was also assessed using a monoclonal anti-PVYN. Although a duration of 2 min was enough for the virus acquisition, the three tested aphids showed different capacities to retain PVYNTN. The retention of PVYNTN was 3 h for M. persicae and A. spiraecola, and 2 h for A. gossypii. This study provides basic information of the virus retention by potato-colonizing aphid species, which may increase our understanding of PVY epidemiology in Tunisia.
Disciplines :
Entomology & pest control Agriculture & agronomy
Author, co-author :
Boukhris-Bouhachem, Sonia; Plant Protection Laboratory of National Institute of Agricultural Research of Tunisia, Ariana, Tunisia
Ben Fekih, Ibtissem ; Université de Liège - ULiège > Département GxABT > Gestion durable des bio-agresseurs ; Plant Protection Laboratory of National Institute of Agricultural Research of Tunisia, Ariana, Tunisia
Nahdi, Sabrine; Dry Land Farming and Oasis Cropping Laboratory, Arid Land Institute, El Fjè-Medenine, Tunisia
Souissi, Rebha; Plant Protection Laboratory of National Institute of Agricultural Research of Tunisia, Ariana, Tunisia
Language :
English
Title :
Capacity assessment of Myzus persicae, Aphis gossypii and Aphis spiraecola (Hemiptera: Aphididae) to acquire and retain PVYNTN in Tunisia
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