[en] Tartary buckwheat, valued for its nutritious and medicinal quercetin. Following two independent domestication events, distinct quercetin accumulation patterns have emerged between the southwestern (SL) and northern (NL) landrace populations. However, the genetic mechanisms underlying these metabolic divergences remain elusive. Here, we identified the transcription factor FtNAC2 through genome-wide association study (GWAS) of quercetin content in 480 accessions of Tartary buckwheat. Haplotype analysis identified two single nucleotide polymorphisms (SNPs) in the FtNAC2 promoter that defined three major haplotypes, with higher promoter activity and gene expression observed in Hap 2. Functional characterization revealed that FtNAC2 promotes quercetin accumulation in Tartary buckwheat hairy roots and potentially serves as a multifunctional regulator influencing both drought tolerance in buckwheat and seed size in Arabidopsis. Transcriptome co-clustering and pulldown mass spectrometry (MS) indicated FtNAC52 as a potential regulatory partner of FtNAC2. DNA affinity purification sequencing (DAP-seq) and quantitative reverse transcription PCR (qRT-PCR) analyses demonstrated that FtNAC2 promoted quercetin biosynthesis by upregulating FtF3'H and FtF3'5'H genes. Collectively, our results elucidated how FtNAC2 influences quercetin content variation in Tartary buckwheat, providing molecular insights into the differential quercetin accumulation between cultivated populations.
Disciplines :
Agriculture & agronomy Chemistry
Author, co-author :
Wang, Jing ; Université de Liège - ULiège > TERRA Research Centre ; National Key Facility for Crop Gene Resources and Genetic Improvement Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing, China ; Sanya Nan Fan Research Institute, Chinese Academy of Agricultural Sciences, Sanya, Hainan, China
Li, Wei; National Key Facility for Crop Gene Resources and Genetic Improvement Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing, China ; Sanya Nan Fan Research Institute, Chinese Academy of Agricultural Sciences, Sanya, Hainan, China
Fan, Dongqing; National Key Facility for Crop Gene Resources and Genetic Improvement Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing, China ; Sanya Nan Fan Research Institute, Chinese Academy of Agricultural Sciences, Sanya, Hainan, China
He, Yuqi; National Key Facility for Crop Gene Resources and Genetic Improvement Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing, China ; Sanya Nan Fan Research Institute, Chinese Academy of Agricultural Sciences, Sanya, Hainan, China
Shi, Yaliang; National Key Facility for Crop Gene Resources and Genetic Improvement Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing, China ; Sanya Nan Fan Research Institute, Chinese Academy of Agricultural Sciences, Sanya, Hainan, China
Lin, Hao; National Key Facility for Crop Gene Resources and Genetic Improvement Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing, China ; Sanya Nan Fan Research Institute, Chinese Academy of Agricultural Sciences, Sanya, Hainan, China
Fauconnier, Marie-Laure ; Université de Liège - ULiège > Département GxABT > Chemistry for Sustainable Food and Environmental Systems (CSFES)
Purcaro, Giorgia ; Université de Liège - ULiège > Département GxABT > Chemistry for Sustainable Food and Environmental Systems (CSFES)
Quinet, Muriel; Groupe de Recherche en Physiologie Végétale (GRPV) Earth and Life Institute-Agronomy (ELI-A), Université Catholique de Louvain Croix du Sud 45, Louvain -la-Neuve, Belgium
Genva, Manon ; Université de Liège - ULiège > Département GxABT > Chemistry for Sustainable Food and Environmental Systems (CSFES)
Zhang, Kaixuan; National Key Facility for Crop Gene Resources and Genetic Improvement Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing, China ; Sanya Nan Fan Research Institute, Chinese Academy of Agricultural Sciences, Sanya, Hainan, China
Ding, Mengqi; College of Agronomy and Biotechnology, Southwest University, Chongqing, 400715 China
Zhou, Meiliang; National Key Facility for Crop Gene Resources and Genetic Improvement Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing, China ; Sanya Nan Fan Research Institute, Chinese Academy of Agricultural Sciences, Sanya, Hainan, China
Natural variation in the FtNAC2 promoter regulates quercetin accumulation and drought tolerance in Tartary buckwheat
Publication date :
2025
Journal title :
Plant, Cell and Environment
ISSN :
0140-7791
eISSN :
1365-3040
Publisher :
Blackwell, Oxford, United Kingdom
Volume :
2025
Pages :
1-17
Peer reviewed :
Peer Reviewed verified by ORBi
Funding text :
This study was supported by the National Key Research and Development Program of China (Grant no. 2023YFD1200701) and the National High-level University Graduate Scholarship Program of the China Scholarship Council (Award no. 202303250057).
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