[en] The presence of macrovitamin B12 (macroB12) in serum inferfers in result interpretation of total vitamin B12 (B12) result and can lead to misidentification of deficiency. Polyethylene glycol (PEG) precipitation is a non-specific method that removes macromolecules (protein polymers and antibody-bound analytes) allowing measurment of circulating vitamin B12 (i.e. B12 bound to transcobalamin and haptocorrin).
Research Center/Unit :
Department of Clinical Chemistry, CHU de Liège
Disciplines :
Laboratory medicine & medical technology
Author, co-author :
Vranken, Laura ; Centre Hospitalier Universitaire de Liège - CHU > > Secteur commun NDB
DIEUPE, Grace ; CHU Liège - Centre Hospitalier Universitaire de Liège > Biologie Clinique > Chimie clinique
Cavalier, Etienne ; Centre Hospitalier Universitaire de Liège - CHU > > Service de chimie clinique
Language :
English
Title :
Comparison of three PEG precipitation protocols for the detection of macrovitamin B12 interference
Publication date :
13 November 2025
Event name :
Annual meeting of the Royal Society of Laboratory Medecine 2025
